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Data and Resources for CNGA4 Gene

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CNGA4-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGAGCCAGGACACCAAAGTGAAGACAACAGAGTCCAGTCCCCCAGCCCCATCCAAGGCCAGGAAGTTGCTGCCTGTCCT
GGACCCATCTGGGGATTACTACTACTGGTGGCTGAACACAATGGTCTTCCCAGTCATGTATAACCTCATCATCCTCGTGT
GCAGAGCCTGCTTCCCCGACTTGCAGCACGGTTATCTGGTGGCCTGGTTGGTGCTGGACTACACGAGTGACCTGCTATAC
CTACTAGACATGGTGGTGCGCTTCCACACAGGATTCTTGGAACAGGGCATCCTGGTGGTGGACAAGGGTAGGATCTCGAG
TCGCTACGTTCGCACCTGGAGTTTCTTCTTGGACCTGGCTTCCCTGATGCCCACAGATGTGGTCTACGTGCGGCTGGGCC
CGCACACACCCACCCTGAGGCTGAACCGCTTTCTCCGCGCGCCCCGCCTCTTCGAGGCCTTCGACCGCACAGAGACCCGC
ACAGCTTACCCAAATGCCTTTCGCATTGCCAAGCTGATGCTTTACATTTTTGTCGTCATCCATTGGAACAGCTGCCTATA
CTTTGCCCTATCCCGGTACCTGGGCTTCGGGCGTGACGCATGGGTGTACCCGGACCCCGCGCAGCCTGGCTTTGAGCGCC
TGCGGCGCCAGTACCTCTATAGCTTTTACTTCTCCACGCTGATACTGACTACAGTGGGCGATACACCGCCGCCAGCCAGG
GAAGAAGAGTACCTCTTCATGGTGGGCGACTTCCTGCTGGCCGTCATGGGTTTCGCCACCATCATGGGTAGCATGAGCTC
TGTCATCTACAACATGAACACTGCAGATGCGGCTTTCTACCCAGATCATGCACTGGTGAAGAAGTACATGAAGCTGCAGC
ACGTCAACCGCAAGCTGGAGCGGCGAGTTATTGACTGGTATCAGCACCTGCAGATCAACAAGAAGATGACCAACGAGGTA
GCCATCTTACAGCACTTGCCTGAGCGGCTGCGGGCAGAAGTGGCTGTGTCTGTGCACCTGTCCACTCTGAGCCGGGTGCA
GATCTTTCAGAACTGTGAGGCCAGCCTGCTGGAGGAGCTGGTGCTGAAGCTGCAGCCCCAGACCTACTCACCAGGTGAAT
ATGTATGCCGCAAAGGAGACATTGGCCAAGAGATGTACATCATCCGAGAGGGTCAACTGGCCGTGGTGGCAGATGATGGT
ATCACACAGTATGCTGTGCTCGGTGCAGGGCTCTACTTTGGGGAGATCAGCATCATCAACATCAAAGGGAACATGTCTGG
GAACCGCCGCACAGCCAACATCAAGAGCCTAGGTTATTCAGACCTATTCTGCCTGAGCAAGGAGGACCTGCGGGAGGTGC
TGAGCGAGTATCCACAAGCACAGACCATCATGGAGGAGAAAGGACGTGAGATCCTGCTGAAAATGAACAAGTTGGACGTG
AATGCTGAGGCAGCTGAGATCGCCCTGCAGGAGGCCACAGAGTCCCGGCTACGAGGCCTAGACCAGCAGCTGGATGATCT
ACAGACCAAGTTTGCTCGCCTCCTGGCTGAGCTGGAGTCCAGCGCACTTAAGATTGCTTACCGCATTGAACGGCTGGAGT
GGCAGACTCGAGAGTGGCCAATGCCCGAGGACCTGGCTGAGGCTGATGACGAGGGTGAGCCTGAGGAGGGAACTTCCAAA
GATGAAGAGGGCAGGGCCAGCCAGGAGGGACCCCCAGGTCCAGAGTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCC
TATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_CNGA4_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGAGCCAGGACACCAAAGTGAAGACAACAGAGTCCAGTCCCCCAGCCCCATCCAAGGCCAGGAAGTTGCTGCCTGTCCT
GGACCCATCTGGGGATTACTACTACTGGTGGCTGAACACAATGGTCTTCCCAGTCATGTATAACCTCATCATCCTCGTGT
GCAGAGCCTGCTTCCCCGACTTGCAGCACGGTTATCTGGTGGCCTGGTTGGTGCTGGACTACACGAGTGACCTGCTATAC
CTACTAGACATGGTGGTGCGCTTCCACACAGGATTCTTGGAACAGGGCATCCTGGTGGTGGACAAGGGTAGGATCTCGAG
TCGCTACGTTCGCACCTGGAGTTTCTTCTTGGACCTGGCTTCCCTGATGCCCACAGATGTGGTCTACGTGCGGCTGGGCC
CGCACACACCCACCCTGAGGCTGAACCGCTTTCTCCGCGCGCCCCGCCTCTTCGAGGCCTTCGACCGCACAGAGACCCGC
ACAGCTTACCCAAATGCCTTTCGCATTGCCAAGCTGATGCTTTACATTTTTGTCGTCATCCATTGGAACAGCTGCCTATA
CTTTGCCCTATCCCGGTACCTGGGCTTCGGGCGTGACGCATGGGTGTACCCGGACCCCGCGCAGCCTGGCTTTGAGCGCC
TGCGGCGCCAGTACCTCTATAGCTTTTACTTCTCCACGCTGATACTGACTACAGTGGGCGATACACCGCCGCCAGCCAGG
GAAGAAGAGTACCTCTTCATGGTGGGCGACTTCCTGCTGGCCGTCATGGGTTTCGCCACCATCATGGGTAGCATGAGCTC
TGTCATCTACAACATGAACACTGCAGATGCGGCTTTCTACCCAGATCATGCACTGGTGAAGAAGTACATGAAGCTGCAGC
ACGTCAACCGCAAGCTGGAGCGGCGAGTTATTGACTGGTATCAGCACCTGCAGATCAACAAGAAGATGACCAACGAGGTA
GCCATCTTACAGCACTTGCCTGAGCGGCTGCGGGCAGAAGTGGCTGTGTCTGTGCACCTGTCCACTCTGAGCCGGGTGCA
GATCTTTCAGAACTGTGAGGCCAGCCTGCTGGAGGAGCTGGTGCTGAAGCTGCAGCCCCAGACCTACTCACCAGGTGAAT
ATGTATGCCGCAAAGGAGACATTGGCCAAGAGATGTACATCATCCGAGAGGGTCAACTGGCCGTGGTGGCAGATGATGGT
ATCACACAGTATGCTGTGCTCGGTGCAGGGCTCTACTTTGGGGAGATCAGCATCATCAACATCAAAGGGAACATGTCTGG
GAACCGCCGCACAGCCAACATCAAGAGCCTAGGTTATTCAGACCTATTCTGCCTGAGCAAGGAGGACCTGCGGGAGGTGC
TGAGCGAGTATCCACAAGCACAGACCATCATGGAGGAGAAAGGACGTGAGATCCTGCTGAAAATGAACAAGTTGGACGTG
AATGCTGAGGCAGCTGAGATCGCCCTGCAGGAGGCCACAGAGTCCCGGCTACGAGGCCTAGACCAGCAGCTGGATGATCT
ACAGACCAAGTTTGCTCGCCTCCTGGCTGAGCTGGAGTCCAGCGCACTTAAGATTGCTTACCGCATTGAACGGCTGGAGT
GGCAGACTCGAGAGTGGCCAATGCCCGAGGACCTGGCTGAGGCTGATGACGAGGGTGAGCCTGAGGAGGGAACTTCCAAA
GATGAAGAGGGCAGGGCCAGCCAGGAGGGACCCCCAGGTCCAGAGTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCC
TATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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