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Data and Resources for KCNAB2 Gene

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNAB2-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

 

Single cell clones: A5, G8

Subcellular localization: Membrane + Cytoplasm

Sequence

ORF Sequence:

ATGTATCCAGAATCAACGACGGGCTCCCCGGCTCGGCTCTCGCTGCGGCAGACGGGCTCCCCCGGGATGATCTACAGTAC TCGGTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAACCTGGGCAAGTCTGGCCTGCGGGTCTCCTGCCTGGGAC TTGGAACATGGGTGACCTTCGGAGGCCAGATCACCGATGAGATGGCAGAGCAGCTCATGACCTTGGCCTATGATAATGGC ATCAACCTCTTCGATACAGCAGAAGTCTACGCAGCCGGCAAGGCTGAAGTGGTACTGGGAAACATCATTAAGAAGAAAGG ATGGAGGCGGTCCAGCCTCGTCATCACCACCAAGATCTTCTGGGGCGGAAAGGCGGAGACGGAGCGGGGCCTGTCCAGGA AGCACATAATCGAAGGTCTGAAAGCTTCCCTGGAGCGACTGCAGCTGGAGTACGTGGATGTGGTGTTTGCCAACCGCCCG GACCCCAACACCCCGATGGAAGAGACCGTCCGCGCCATGACCCACGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC GTCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCCGTGGCCCGGCAGTTCAACCTGACCCCGCCCATCTGCGAGC AGGCTGAGTACCACATGTTCCAGCGTGAGAAAGTGGAGGTGCAGCTGCCGGAGCTGTTCCACAAGATAGGAGTGGGCGCC ATGACCTGGTCCCCTCTGGCCTGTGGCATTGTTTCTGGCAAGTACGACAGTGGCATCCCACCCTACTCAAGAGCCTCCTT GAAGGGCTACCAGTGGCTGAAGGACAAGATCCTCAGTGAGGAGGGCCGGCGCCAGCAAGCCAAGCTGAAGGAGCTGCAGG CCATCGCCGAGCGCCTGGGCTGCACCCTGCCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGAGTCAGCTCCGTG CTCCTGGGGGCCTCCAATGCGGACCAGCTCATGGAGAACATTGGGGCAATACAGGTCCTTCCGAAACTGTCGTCTTCCAT TATCCACGAGATTGATAGTATTTTGGGCAATAAACCCTACAGCAAAAAGGACTACAGATCCTTGCCAACTTTCTTGTACA AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNAB2_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGTATCCAGAATCAACGACGGGCTCCCCGGCTCGGCTCTCGCTGCGGCAGACGGGCTCCCCCGGGATGATCTACAGTAC
TCGGTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAACCTGGGCAAGTCTGGCCTGCGGGTCTCCTGCCTGGGAC
TTGGAACATGGGTGACCTTCGGAGGCCAGATCACCGATGAGATGGCAGAGCAGCTCATGACCTTGGCCTATGATAATGGC
ATCAACCTCTTCGATACAGCAGAAGTCTACGCAGCCGGCAAGGCTGAAGTGGTACTGGGAAACATCATTAAGAAGAAAGG
ATGGAGGCGGTCCAGCCTCGTCATCACCACCAAGATCTTCTGGGGCGGAAAGGCGGAGACGGAGCGGGGCCTGTCCAGGA
AGCACATAATCGAAGGTCTGAAAGCTTCCCTGGAGCGACTGCAGCTGGAGTACGTGGATGTGGTGTTTGCCAACCGCCCG
GACCCCAACACCCCGATGGAAGAGACCGTCCGCGCCATGACCCACGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC
GTCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCCGTGGCCCGGCAGTTCAACCTGACCCCGCCCATCTGCGAGC
AGGCTGAGTACCACATGTTCCAGCGTGAGAAAGTGGAGGTGCAGCTGCCGGAGCTGTTCCACAAGATAGGAGTGGGCGCC
ATGACCTGGTCCCCTCTGGCCTGTGGCATTGTTTCTGGCAAGTACGACAGTGGCATCCCACCCTACTCAAGAGCCTCCTT
GAAGGGCTACCAGTGGCTGAAGGACAAGATCCTCAGTGAGGAGGGCCGGCGCCAGCAAGCCAAGCTGAAGGAGCTGCAGG
CCATCGCCGAGCGCCTGGGCTGCACCCTGCCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGAGTCAGCTCCGTG
CTCCTGGGGGCCTCCAATGCGGACCAGCTCATGGAGAACATTGGGGCAATACAGGTCCTTCCGAAACTGTCGTCTTCCAT
TATCCACGAGATTGATAGTATTTTGGGCAATAAACCCTACAGCAAAAAGGACTACAGATCCTTGCCAACTTTCTTGTACA
AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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