https://darkmatter.ucsf.edu/ en https://darkmatter.ucsf.edu/data-mining/disease-data-lrrc55-0 <span class="field field--name-title field--type-string field--label-hidden">Disease Data for LRRC55</span> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span lang="" about="/user/11" typeof="schema:Person" property="schema:name" datatype="">ctran</span></span> <span class="field field--name-created field--type-created field--label-hidden">Tue, 03/16/2021 - 14:05</span> <div class="field field--name-field-10k-immunome field--type-entity-reference field--label-above"> <div class="field__label">10K Immunome:</div> <div class="field__item"><a href="/taxonomy/term/3556" hreflang="en">Not Detected</a></div> </div> <div class="field field--name-field-sars-cov-2-association field--type-entity-reference field--label-above"> <div class="field__label">SARS-CoV-2 Association:</div> <div class="field__item"><a href="/taxonomy/term/3561" hreflang="en">Not Detected</a></div> </div> <div class="field field--name-field-sars-cov-2-vaccine-associa field--type-entity-reference field--label-above"> <div class="field__label">SARS-CoV-2 Vaccine Association:</div> <div class="field__item"><a href="/taxonomy/term/3566" hreflang="en">Not Detected</a></div> </div> <div class="field field--name-field-gene-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Gene Taxonomy:</div> <div class="field__item"><a href="/gene/lrrc55" hreflang="en">LRRC55</a></div> </div> <div class="field field--name-field-uniprotid field--type-string field--label-inline"> <div class="field__label">UniprotID:</div> <div class="field__item">Q6ZSA7</div> </div> <div class="field field--name-field-mouse-homolog field--type-string field--label-inline"> <div class="field__label">Mouse Homolog:</div> <div class="field__item">Lrrc55</div> </div> <div class="field field--name-field-pharos-link field--type-link field--label-inline"> <div class="field__label">Pharos Link:</div> <div class="field__item"><a href="https://pharos.nih.gov/targets/Q6ZSA7">Pharos Link</a></div> </div> <div class="field field--name-field-knockout-mouse-phenotype field--type-entity-reference field--label-inline"> <div class="field__label">Knockout Mouse Phenotype:</div> <div class="field__items"> <div class="field__item"><a href="/taxonomy/term/3501" hreflang="x-default">Not Detected</a></div> </div> </div> <div class="field field--name-field-monogenic-disease-associat field--type-entity-reference field--label-inline"> <div class="field__label">Monogenic Disease Association:</div> <div class="field__items"> <div class="field__item"><a href="/taxonomy/term/3496" hreflang="x-default">Not Detected</a></div> </div> </div> <div class="field field--name-field-polygenic-disease-associat field--type-entity-reference field--label-inline"> <div class="field__label">Polygenic Disease Association:</div> <div class="field__items"> <div class="field__item"><a href="/polygenic-disease-association/not-detected" hreflang="x-default">Not Detected</a></div> </div> </div> Tue, 16 Mar 2021 21:05:38 +0000 ctran 14931 at https://darkmatter.ucsf.edu https://darkmatter.ucsf.edu/data-mining/disease-data-lrrc55 <span class="field field--name-title field--type-string field--label-hidden">Disease Data for LRRC55</span> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span lang="" about="/user/11" typeof="schema:Person" property="schema:name" datatype="">ctran</span></span> <span class="field field--name-created field--type-created field--label-hidden">Tue, 03/16/2021 - 12:45</span> <div class="field field--name-field-10k-immunome field--type-entity-reference field--label-above"> <div class="field__label">10K Immunome:</div> <div class="field__item"><a href="/taxonomy/term/3556" hreflang="en">Not Detected</a></div> </div> <div class="field field--name-field-sars-cov-2-association field--type-entity-reference field--label-above"> <div class="field__label">SARS-CoV-2 Association:</div> <div class="field__item"><a href="/taxonomy/term/3561" hreflang="en">Not Detected</a></div> </div> <div class="field field--name-field-sars-cov-2-vaccine-associa field--type-entity-reference field--label-above"> <div class="field__label">SARS-CoV-2 Vaccine Association:</div> <div class="field__item"><a href="/taxonomy/term/3566" hreflang="en">Not Detected</a></div> </div> <div class="field field--name-field-gene-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Gene Taxonomy:</div> <div class="field__item"><a href="/gene/lrrc55" hreflang="en">LRRC55</a></div> </div> <div class="field field--name-field-uniprotid field--type-string field--label-inline"> <div class="field__label">UniprotID:</div> <div class="field__item">Q6ZSA7</div> </div> <div class="field field--name-field-mouse-homolog field--type-string field--label-inline"> <div class="field__label">Mouse Homolog:</div> <div class="field__item">Lrrc55</div> </div> <div class="field field--name-field-pharos-link field--type-link field--label-inline"> <div class="field__label">Pharos Link:</div> <div class="field__item"><a href="https://pharos.nih.gov/targets/Q6ZSA7">Pharos Link</a></div> </div> <div class="field field--name-field-knockout-mouse-phenotype field--type-entity-reference field--label-inline"> <div class="field__label">Knockout Mouse Phenotype:</div> <div class="field__items"> <div class="field__item"><a href="/taxonomy/term/3501" hreflang="x-default">Not Detected</a></div> </div> </div> <div class="field field--name-field-monogenic-disease-associat field--type-entity-reference field--label-inline"> <div class="field__label">Monogenic Disease Association:</div> <div class="field__items"> <div class="field__item"><a href="/taxonomy/term/3496" hreflang="x-default">Not Detected</a></div> </div> </div> <div class="field field--name-field-polygenic-disease-associat field--type-entity-reference field--label-inline"> <div class="field__label">Polygenic Disease Association:</div> <div class="field__items"> <div class="field__item"><a href="/polygenic-disease-association/not-detected" hreflang="x-default">Not Detected</a></div> </div> </div> Tue, 16 Mar 2021 19:45:49 +0000 ctran 14830 at https://darkmatter.ucsf.edu https://darkmatter.ucsf.edu/cells/idg-hek293t-lrrc55-v5-oe <span class="field field--name-title field--type-string field--label-hidden">Over Expression Cells for LRRC55</span> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span lang="" about="/user/11" typeof="schema:Person" property="schema:name" datatype="">ctran</span></span> <span class="field field--name-created field--type-created field--label-hidden">Thu, 03/11/2021 - 13:22</span> <div class="clearfix text-formatted field field--name-field-orf-sequence field--type-text-long field--label-above"> <div class="field__label">ORF Sequence:</div> <div class="field__item"><p>ATGCTCAGAAGCCCCACCTTCACAGACGCTGGCCCCAGATGTAGCTGCCTTCCTGTGTCACAGACTCTCGATTCCATGGA CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG</p> </div> </div> <div class="field field--name-field-parent-cell-line field--type-string field--label-inline"> <div class="field__label">Parent Cell Line:</div> <div class="field__item">HEK293</div> </div> <div class="field field--name-field-gene-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Gene Taxonomy:</div> <div class="field__item"><a href="/gene/lrrc55" hreflang="en">LRRC55</a></div> </div> <div class="field field--name-field-cell-line-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Cell Line Taxonomy:</div> <div class="field__items"> <div class="field__item"><a href="/taxonomy/term/611" hreflang="en">293T</a></div> <div class="field__item"><a href="/taxonomy/term/606" hreflang="en">Over Expression Cell Line</a></div> </div> </div> <div class="field field--name-field-cell-type field--type-string field--label-inline"> <div class="field__label">Cell Type:</div> <div class="field__item">Stable overexpression cell line</div> </div> <div class="field field--name-field-cell-id field--type-string field--label-inline"> <div class="field__label">Cell ID:</div> <div class="field__item">IDG-HEK293T-LRRC55-V5-OE</div> </div> <div class="field field--name-field-resistance-selection field--type-string field--label-inline"> <div class="field__label">Selection:</div> <div class="field__item">Blasticidin to a final concentration of 10μg/m</div> </div> <div class="field field--name-field-organism field--type-string field--label-inline"> <div class="field__label">Organism:</div> <div class="field__item">Homo sapiens, human</div> </div> <div class="clearfix text-formatted field field--name-field-description field--type-text-with-summary field--label-above"> <div class="field__label">Description:</div> <div class="field__item"><p>This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.</p> <p> </p> <p>Single cell clone: D5</p> <p>Subcellular localization: Intracellular + perinuclear</p> </div> </div> <div class="field field--name-field-image field--type-image field--label-hidden field__item"> <a href="https://darkmatter.ucsf.edu/sites/darkmatter.ucsf.edu/files/2019-08/rowf.lif_LRRC55_f9_3_dapi_Merged001.png" aria-controls="colorbox" aria-label="{&quot;alt&quot;:&quot;&quot;}" role="button" title="" data-colorbox-gallery="gallery-all-ZynxkRfICbA" class="colorbox" data-cbox-img-attrs="{&quot;alt&quot;:&quot;&quot;}"><img src="/sites/darkmatter.ucsf.edu/files/styles/medium/public/2019-08/rowf.lif_LRRC55_f9_3_dapi_Merged001.png?itok=cap5emfx" width="220" height="220" alt="" loading="lazy" typeof="foaf:Image" class="image-style-medium" /> </a> </div> <div class="field field--name-field-clonal-line-1 field--type-image field--label-hidden field__item"> <a href="https://darkmatter.ucsf.edu/sites/darkmatter.ucsf.edu/files/2023-03/LRRC_63X_D5.png" aria-controls="colorbox" aria-label="{&quot;title&quot;:&quot;63X image of clone D5&quot;,&quot;alt&quot;:&quot;63X image of clone D5&quot;}" role="button" title="63X image of clone D5" data-colorbox-gallery="gallery-all-ZynxkRfICbA" class="colorbox" data-cbox-img-attrs="{&quot;title&quot;:&quot;63X image of clone D5&quot;,&quot;alt&quot;:&quot;63X image of clone D5&quot;}"><img src="/sites/darkmatter.ucsf.edu/files/styles/medium/public/2023-03/LRRC_63X_D5.png?itok=0C5pB2xu" width="220" height="220" alt="63X image of clone D5" title="63X image of clone D5" loading="lazy" typeof="foaf:Image" class="image-style-medium" /> </a> </div> Thu, 11 Mar 2021 21:22:05 +0000 ctran 13021 at https://darkmatter.ucsf.edu https://darkmatter.ucsf.edu/constructs/idg_lrrc55_oe_1 <span class="field field--name-title field--type-string field--label-hidden">Lentiviral Construct for LRRC55</span> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span lang="" about="/user/16" typeof="schema:Person" property="schema:name" datatype="">zicheng</span></span> <span class="field field--name-created field--type-created field--label-hidden">Wed, 10/30/2019 - 11:24</span> <div class="field field--name-field-image field--type-image field--label-hidden field__item"> <a href="https://darkmatter.ucsf.edu/sites/darkmatter.ucsf.edu/files/2021-03/D.IC81-pLX304-LRRC55-expression-vector%20Map.png" aria-controls="colorbox" aria-label="{&quot;alt&quot;:&quot;&quot;}" role="button" title="Lentiviral Construct for LRRC55" data-colorbox-gallery="gallery-dna_constructs-10041-ZynxkRfICbA" class="colorbox" data-cbox-img-attrs="{&quot;alt&quot;:&quot;&quot;}"><img src="/sites/darkmatter.ucsf.edu/files/styles/medium/public/2021-03/D.IC81-pLX304-LRRC55-expression-vector%20Map.png?itok=SbRzboBV" width="215" height="220" alt="" loading="lazy" typeof="foaf:Image" class="image-style-medium" /> </a> </div> <div class="field field--name-field-inserted-sequence field--type-string field--label-above"> <div class="field__label">Inserted_sequence:</div> <div class="field__item">ATGCTCAGAAGCCCCACCTTCACAGACGCTGGCCCCAGATGTAGCTGCCTTCCTGTGTCACAGACTCTCGATTCCATGGA<br /> CACAGTCCTCATGGGCTCCCTCCAGCACTGCTGTTGCCTGCTGCCTAAGATGGGTGACACTTGGGCCCAGCTTCCCTGGC<br /> CCGGGCCACCCCACCCAGCAATGCTGCTGATCTCCCTCCTCTTGGCAGCCGGGTTGATGCACTCGGATGCCGGCACCAGC<br /> TGCCCCGTCCTTTGCACATGCCGTAACCAGGTGGTGGATTGTAGCAGCCAGCGGCTATTCTCCGTGCCCCCAGACCTGCC<br /> AATGGACACCCGAAACCTCAGCCTGGCCCACAACCGCATCACAGCAGTGCCGCCTGGCTACCTCACATGCTACATGGAGC<br /> TCCAGGTGCTGGATTTGCACAACAACTCCTTAATGGAGCTGCCCCGGGGCCTCTTCCTCCATGCCAAGCGCTTGGCACAC<br /> TTGGACCTGAGCTACAACAATTTCAGCCATGTGCCAGCCGACATGTTCCAGGAGGCCCATGGGCTAGTCCACATCGACCT<br /> GAGCCACAACCCCTGGCTGCGGAGGGTGCATCCCCAGGCCTTTCAGGGCCTCATGCAGCTCCGAGACCTGGACCTCAGTT<br /> ATGGGGGCCTGGCCTTCCTCAGCCTGGAGGCTCTTGAGGGCCTACCGGGGCTGGTGACCCTGCAGATCGGTGGCAATCCC<br /> TGGGTGTGTGGCTGCACCATGGAACCCCTGCTGAAGTGGCTGCGAAACCGGATCCAGCGCTGTACAGCAGATTCTCAGCT<br /> GGCTGAGTGCCGGGGCCCTCCTGAAGTCGAGGGCGCCCCGCTCTTCTCACTCACTGAGGAGAGCTTCAAGGCCTGCCACC<br /> TGACCCTGACCCTGGATGATTACCTATTCATTGCGTTTGTGGGCTTCGTGGTCTCCATTGCTTCTGTGGCCACCAACTTC<br /> CTCCTGGGCATCACTGCCAACTGCTGCCACCGCTGGAGCAAGGCCAGTGAAGAGGAAGAGATCTTGCCAACTTTCTTGTA<br /> CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG<br /> <br /> </div> </div> <div class="field field--name-field-snapgene-file field--type-file field--label-above"> <div class="field__label">Snapgene File:</div> <div class="field__item"> <span class="file file--mime-application-octet-stream file--general"> <a href="/sites/darkmatter.ucsf.edu/files/2021-03/D.IC81-pLX304-LRRC55-expression-vector.dna" type="application/octet-stream">D.IC81-pLX304-LRRC55-expression-vector.dna</a></span> </div> </div> <div class="field field--name-field-gene-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Gene Taxonomy:</div> <div class="field__item"><a href="/gene/lrrc55" hreflang="en">LRRC55</a></div> </div> <div class="field field--name-field-virus-type field--type-entity-reference field--label-inline"> <div class="field__label">Virus Type:</div> <div class="field__item"><a href="/taxonomy/term/3511" hreflang="en">Lentiviral Vector</a></div> </div> <div class="field field--name-field-vector-type field--type-entity-reference field--label-inline"> <div class="field__label">Vector Type:</div> <div class="field__item"><a href="/taxonomy/term/3506" hreflang="en">cDNA</a></div> </div> <div class="field field--name-field-title field--type-string field--label-inline"> <div class="field__label">Construct ID:</div> <div class="field__item">IDG_LRRC55_OE_1<br /> <br /> </div> </div> <div class="field field--name-field-selection-txt field--type-string field--label-inline"> <div class="field__label">Selection:</div> <div class="field__item">Amp, Blasticidin<br /> <br /> </div> </div> <div class="field field--name-field-vector-txt field--type-string field--label-inline"> <div class="field__label">Vector:</div> <div class="field__item">lPX304<br /> <br /> </div> </div> <div class="field field--name-field-construct-type field--type-string field--label-inline"> <div class="field__label">Construct Type:</div> <div class="field__item">Expression<br /> <br /> </div> </div> <div class="field field--name-field-tag field--type-string field--label-inline"> <div class="field__label">Tag:</div> <div class="field__item">C terminal V5<br /> <br /> </div> </div> <div class="field field--name-field-construct-passed-qc field--type-string field--label-inline"> <div class="field__label">Passed QC:</div> <div class="field__item">Yes<br /> <br /> </div> </div> Wed, 30 Oct 2019 18:24:46 +0000 zicheng 10041 at https://darkmatter.ucsf.edu https://darkmatter.ucsf.edu/crispri/idg_lrrc55_ue_1 <span class="field field--name-title field--type-string field--label-hidden">CRISPRI Construct for LRRC55</span> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span lang="" about="/user/11" typeof="schema:Person" property="schema:name" datatype="">ctran</span></span> <span class="field field--name-created field--type-created field--label-hidden">Mon, 09/23/2019 - 14:11</span> <div class="field field--name-field-gene-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Gene Taxonomy:</div> <div class="field__item"><a href="/gene/lrrc55" hreflang="en">LRRC55</a></div> </div> <div class="field field--name-field-image field--type-image field--label-above"> <div class="field__label">Vector Map:</div> <div class="field__item"> <a href="https://darkmatter.ucsf.edu/sites/darkmatter.ucsf.edu/files/2021-03/G3-783-LRRC55-interference-vector%20Map.png" aria-controls="colorbox" aria-label="{&quot;alt&quot;:&quot;&quot;}" role="button" title="CRISPRI Construct for LRRC55" data-colorbox-gallery="gallery-crispri_construct-6931-ZynxkRfICbA" class="colorbox" data-cbox-img-attrs="{&quot;alt&quot;:&quot;&quot;}"><img src="/sites/darkmatter.ucsf.edu/files/styles/medium/public/2021-03/G3-783-LRRC55-interference-vector%20Map.png?itok=fdC7O7T7" width="220" height="203" alt="" loading="lazy" typeof="foaf:Image" class="image-style-medium" /> </a> </div> </div> <div class="field field--name-field-inserted-sequence field--type-string field--label-inline"> <div class="field__label">Inserted_sequence:</div> <div class="field__item">GCCCAGGTGTCACCCATCTT</div> </div> <div class="field field--name-field-snapgene-file field--type-file field--label-above"> <div class="field__label">Snapgene File:</div> <div class="field__item"> <span class="file file--mime-application-octet-stream file--general"> <a href="/sites/darkmatter.ucsf.edu/files/2021-03/G3-783-LRRC55-interference-vector.dna" type="application/octet-stream">G3-783-LRRC55-interference-vector.dna</a></span> </div> </div> <div class="field field--name-field-title field--type-string field--label-inline"> <div class="field__label">Construct ID:</div> <div class="field__item">IDG_LRRC55_UE_1</div> </div> <div class="field field--name-field-virus-type field--type-entity-reference field--label-inline"> <div class="field__label">Virus Type:</div> <div class="field__item"><a href="/taxonomy/term/3511" hreflang="en">Lentiviral Vector</a></div> </div> <div class="field field--name-field-selection field--type-list-string field--label-inline"> <div class="field__label">Selection:</div> <div class="field__item">Puro,Amp</div> </div> <div class="field field--name-field-vector field--type-list-string field--label-inline"> <div class="field__label">Vector:</div> <div class="field__item">783_hU6_EGFP</div> </div> <div class="field field--name-field-passed-qc field--type-boolean field--label-inline"> <div class="field__label">Passed QC:</div> <div class="field__item">On</div> </div> <div class="field field--name-field-vector-type field--type-entity-reference field--label-inline"> <div class="field__label">Vector Type:</div> <div class="field__item"><a href="/taxonomy/term/3516" hreflang="en">CRISPR Interference</a></div> </div> Mon, 23 Sep 2019 21:11:33 +0000 ctran 6931 at https://darkmatter.ucsf.edu