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Data and Resources for TMEM63C Gene

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-TMEM63C-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGTCTGCCTCACCAGACGACCTGAGTACAGGGGGAAGGTTACAGAACATGACAGTGGATGAATGCTTCCAGTCTCGGAA
CACCGTCCTCCAGGGGCAGCCCTTTGGGGGTGTCCCCACCGTGCTGTGCCTCAACATCGCCCTGTGGGTGCTCGTCCTTG
TGGTTTACTCCTTCCTCCGGAAAGCTGCGTGGGACTATGGGCGCCTGGCTCTGCTGATACACAATGACAGCCTGACCTCG
CTGATCTATGGGGAGCAGAGCGAGAAGACATCTCCCTCGGAGACTTCCTTGGAGATGGAACGCAGAGACAAGGGATTCTG
TTCCTGGTTCTTCAACAGCATAACAATGAAGGACGAGGATCTGATTAACAAGTGTGGGGACGACGCGCGCATCTACATCG
TGTTCCAGTACCACCTCATCATCTTTGTGCTCATCATCTGTATCCCCTCCCTGGGCATCATTTTGCCCATCAACTATACT
GGATCTGTTCTGGACTGGAGCAGTCACTTTGCTCGGACCACCATTGTCAATGTCTCCACAGAGAGCAAGCTCCTGTGGCT
GCATAGCCTGCTGTCCTTCTTCTACTTCATCACCAACTTCATGTTCATGGCTCATCACTGCCTGGGGTTTGCGCCCAGGA
ATAGCCAAAAGGTCACAAGGACACTAATGATCACCTATGTGCCCAAGGACATTGAAGACCCAGAACTCATCATTAAGCAT
TTTCACGAGGCCTATCCAGGCAGTGTCGTGACAAGAGTCCACTTCTGCTACGACGTCAGGAACCTGATCGACTTGGACGA
TCAGAGGCGCCATGCCATGCGGGGCCGGCTTTTCTATACAGCCAAGGCCAAGAAGACTGGGAAGGTGATGATCAGGATCC
ACCCCTGTGCCCGCCTGTGCTTCTGCAAGTGCTGGACCTGCTTCAAGGAGGTGGATGCAGAGCAGTATTACAGCGAGCTA
GAGGAGCAGCTAACGGACGAGTTCAACGCCGAGCTCAACCGCGTGCCGCTCAAGCGGCTGGACCTGATCTTTGTCACCTT
CCAGGACTCCAGGATGGCCAAGCGTGTCCGTAAGGATTACAAGTATGTCCAGTGTGGTGTGCAACCCCAGCAGTCCTCAG
TGACCACCATCGTCAAATCATATTACTGGAGGGTCACTATGGCCCCACACCCCAAAGACATTATTTGGAAACACCTGTCT
GTCCGCCGCTTCTTTTGGTGGGCCCGCTTTATCGCAATCAACACCTTCCTCTTCTTCCTCTTCTTCTTTCTCACCACGCC
TGCCATCATCATGAACACTATCGACATGTACAACGTCACCCGCCCCATCGAGAAGCTGCAGAACCCAATTGTGACCCAGT
TCTTCCCCTCTGTGATGCTCTGGGGCTTCACAGTGATACTGCCTCTGATTGTCTACTTCTCCGCCTTCCTCGAGGCCCAC
TGGACCAGATCAAGTCAGAATCTGGTCATGGTGCACAAGTGCTACATCTTTCTGGTGTTCATGGTAGTCATTCTGCCCTC
TATGGGACTGACCAGTTTGGATGTCTTTCTCCGCTGGCTCTTTGACATCTACTATCTAGAGCAAGCATCCATCAGGTTCC
AGTGTGTGTTCCTGCCAGACAACGGCGCCTTCTTTGTCAACTACGTGATCACGGCAGCTTTACTTGGCACAGGCATGGAG
CTGCTGCGTCTGGGGTCACTCTTCTGCTACAGCACCCGCCTCTTCTTCTCTAGATCAGAGCCAGAGAGAGTCAACATCAG
AAAGAACCAGGCCATAGACTTCCAGTTTGGGCGTGAGTATGCGTGGATGATGAACGTGTTCAGCGTGGTGATGGCGTACA
GCATCACTTGCCCCATCATTGTGCCTTTTGGGTTGCTCTACCTGTGCATGAAGCACTTGACGGATCGCTATAACATGTAC
TACTCCTTTGCACCCACCAAACTGAACGAGCAGATCCACATGGCTGCCGTCTCCCAGGCCATCTTTGCGCCACTCTTGGG
TCTGTTCTGGATGCTGTTCTTCTCCATCCTGCGGTTGGGTTCTCTCCACGCCATCACCATCTTTTCCCTGTCCACCCTCC
TCATTGCCATGGTGATTGCCTTTGTTGGCATTTTTCTGGGGAAGCTTCGGATGGTTGCCGACTACGAGCCCGAGGAGGAG
GAGATCCAGACAGTGTTTGACATGGAGCCAAGCAGCACCTCCTCCACGCCCACCTCCCTCCTGTATGTGGCCACCGTGCT
GCAAGAACCGGAGTTGAATCTGACCCCCGCCTCCTCCCCAGCCAGGCACACCTATGGCACCATGAACAACCAGCCGGAAG
AGGGAGAAGAAGAGAGTGGTCTGAGGGGCTTTGCGAGGGAGCTAGACTCGGCCCAGTTCCAGGAAGGGCTGGAACTGGAG
GGCCAGAACCAGTACCACTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGA
TTCTACGTAG

Media and Files

Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_TMEM63C_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGTCTGCCTCACCAGACGACCTGAGTACAGGGGGAAGGTTACAGAACATGACAGTGGATGAATGCTTCCAGTCTCGGAA
CACCGTCCTCCAGGGGCAGCCCTTTGGGGGTGTCCCCACCGTGCTGTGCCTCAACATCGCCCTGTGGGTGCTCGTCCTTG
TGGTTTACTCCTTCCTCCGGAAAGCTGCGTGGGACTATGGGCGCCTGGCTCTGCTGATACACAATGACAGCCTGACCTCG
CTGATCTATGGGGAGCAGAGCGAGAAGACATCTCCCTCGGAGACTTCCTTGGAGATGGAACGCAGAGACAAGGGATTCTG
TTCCTGGTTCTTCAACAGCATAACAATGAAGGACGAGGATCTGATTAACAAGTGTGGGGACGACGCGCGCATCTACATCG
TGTTCCAGTACCACCTCATCATCTTTGTGCTCATCATCTGTATCCCCTCCCTGGGCATCATTTTGCCCATCAACTATACT
GGATCTGTTCTGGACTGGAGCAGTCACTTTGCTCGGACCACCATTGTCAATGTCTCCACAGAGAGCAAGCTCCTGTGGCT
GCATAGCCTGCTGTCCTTCTTCTACTTCATCACCAACTTCATGTTCATGGCTCATCACTGCCTGGGGTTTGCGCCCAGGA
ATAGCCAAAAGGTCACAAGGACACTAATGATCACCTATGTGCCCAAGGACATTGAAGACCCAGAACTCATCATTAAGCAT
TTTCACGAGGCCTATCCAGGCAGTGTCGTGACAAGAGTCCACTTCTGCTACGACGTCAGGAACCTGATCGACTTGGACGA
TCAGAGGCGCCATGCCATGCGGGGCCGGCTTTTCTATACAGCCAAGGCCAAGAAGACTGGGAAGGTGATGATCAGGATCC
ACCCCTGTGCCCGCCTGTGCTTCTGCAAGTGCTGGACCTGCTTCAAGGAGGTGGATGCAGAGCAGTATTACAGCGAGCTA
GAGGAGCAGCTAACGGACGAGTTCAACGCCGAGCTCAACCGCGTGCCGCTCAAGCGGCTGGACCTGATCTTTGTCACCTT
CCAGGACTCCAGGATGGCCAAGCGTGTCCGTAAGGATTACAAGTATGTCCAGTGTGGTGTGCAACCCCAGCAGTCCTCAG
TGACCACCATCGTCAAATCATATTACTGGAGGGTCACTATGGCCCCACACCCCAAAGACATTATTTGGAAACACCTGTCT
GTCCGCCGCTTCTTTTGGTGGGCCCGCTTTATCGCAATCAACACCTTCCTCTTCTTCCTCTTCTTCTTTCTCACCACGCC
TGCCATCATCATGAACACTATCGACATGTACAACGTCACCCGCCCCATCGAGAAGCTGCAGAACCCAATTGTGACCCAGT
TCTTCCCCTCTGTGATGCTCTGGGGCTTCACAGTGATACTGCCTCTGATTGTCTACTTCTCCGCCTTCCTCGAGGCCCAC
TGGACCAGATCAAGTCAGAATCTGGTCATGGTGCACAAGTGCTACATCTTTCTGGTGTTCATGGTAGTCATTCTGCCCTC
TATGGGACTGACCAGTTTGGATGTCTTTCTCCGCTGGCTCTTTGACATCTACTATCTAGAGCAAGCATCCATCAGGTTCC
AGTGTGTGTTCCTGCCAGACAACGGCGCCTTCTTTGTCAACTACGTGATCACGGCAGCTTTACTTGGCACAGGCATGGAG
CTGCTGCGTCTGGGGTCACTCTTCTGCTACAGCACCCGCCTCTTCTTCTCTAGATCAGAGCCAGAGAGAGTCAACATCAG
AAAGAACCAGGCCATAGACTTCCAGTTTGGGCGTGAGTATGCGTGGATGATGAACGTGTTCAGCGTGGTGATGGCGTACA
GCATCACTTGCCCCATCATTGTGCCTTTTGGGTTGCTCTACCTGTGCATGAAGCACTTGACGGATCGCTATAACATGTAC
TACTCCTTTGCACCCACCAAACTGAACGAGCAGATCCACATGGCTGCCGTCTCCCAGGCCATCTTTGCGCCACTCTTGGG
TCTGTTCTGGATGCTGTTCTTCTCCATCCTGCGGTTGGGTTCTCTCCACGCCATCACCATCTTTTCCCTGTCCACCCTCC
TCATTGCCATGGTGATTGCCTTTGTTGGCATTTTTCTGGGGAAGCTTCGGATGGTTGCCGACTACGAGCCCGAGGAGGAG
GAGATCCAGACAGTGTTTGACATGGAGCCAAGCAGCACCTCCTCCACGCCCACCTCCCTCCTGTATGTGGCCACCGTGCT
GCAAGAACCGGAGTTGAATCTGACCCCCGCCTCCTCCCCAGCCAGGCACACCTATGGCACCATGAACAACCAGCCGGAAG
AGGGAGAAGAAGAGAGTGGTCTGAGGGGCTTTGCGAGGGAGCTAGACTCGGCCCAGTTCCAGGAAGGGCTGGAACTGGAG
GGCCAGAACCAGTACCACTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGA
TTCTACGTAG

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