https://darkmatter.ucsf.edu/ en https://darkmatter.ucsf.edu/cells/idg-hek293t-calhm4-v5-oe <span class="field field--name-title field--type-string field--label-hidden">Over Expressed Cell Line for CALHM4</span> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span lang="" about="/user/11" typeof="schema:Person" property="schema:name" datatype="">ctran</span></span> <span class="field field--name-created field--type-created field--label-hidden">Thu, 03/11/2021 - 13:22</span> <div class="clearfix text-formatted field field--name-field-orf-sequence field--type-text-long field--label-above"> <div class="field__label">ORF Sequence:</div> <div class="field__item"><p>ATGTTTGATAATGTCAGTGCCAGCAAACGAGAAGAGATCCTAGCTGGGTTTCCATGTTGCAGATCAGCTCCTTCTGACGT<br /> GATCCTAGTAAGAGATGAAATAGCTCTTCTGCACAGATACCAGTCACAGATGCTGGGTTGGATTTTGATCACCTTGGCAA<br /> CCATTGCTGCCTTAGTCTCCTGCTGTGTGGCAAAGTGCTGCTCTCCCCTCACCTCTCTGCAACATTGCTACTGGACCAGC<br /> CACCTCCAGAATGAGAGAGAACTCTTTGAACAAGCAGCAGAGCAGCACTCTCGGCTCCTCATGATGCATCGCATAAAGAA<br /> GCTATTTGGCTTCATTCCCGGGAGTGAAGACGTCAAACACATCCGCATTCCTTCTTGTCAGGACTGGAAAGATATTTCAG<br /> TACCCACTCTTTTATGCATGGGTGATGACTTGCAAGGTCACTATAGCTTCCTTGGAAATAGGGTGGATGAGGATAATGAG<br /> GAAGACAGATCAAGAGGTATTGAATTAAAACCTACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCT<br /> CCTCGGTCTCGATTCTACGTAG</p> </div> </div> <div class="field field--name-field-parent-cell-line field--type-string field--label-inline"> <div class="field__label">Parent Cell Line:</div> <div class="field__item">HEK293</div> </div> <div class="field field--name-field-gene-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Gene Taxonomy:</div> <div class="field__item"><a href="/gene/calhm4" hreflang="en">CALHM4</a></div> </div> <div class="field field--name-field-cell-line-taxonomy field--type-entity-reference field--label-inline"> <div class="field__label">Cell Line Taxonomy:</div> <div class="field__items"> <div class="field__item"><a href="/taxonomy/term/611" hreflang="en">293T</a></div> <div class="field__item"><a href="/taxonomy/term/606" hreflang="en">Over Expression Cell Line</a></div> </div> </div> <div class="field field--name-field-cell-type field--type-string field--label-inline"> <div class="field__label">Cell Type:</div> <div class="field__item">Stable overexpression cell line</div> </div> <div class="field field--name-field-cell-id field--type-string field--label-inline"> <div class="field__label">Cell ID:</div> <div class="field__item">IDG-HEK293T-CALHM4-V5-OE</div> </div> <div class="field field--name-field-resistance-selection field--type-string field--label-inline"> <div class="field__label">Selection:</div> <div class="field__item">Blasticidin to a final concentration of 10μg/m</div> </div> <div class="field field--name-field-organism field--type-string field--label-inline"> <div class="field__label">Organism:</div> <div class="field__item">Homo sapiens, human</div> </div> <div class="clearfix text-formatted field field--name-field-description field--type-text-with-summary field--label-above"> <div class="field__label">Description:</div> <div class="field__item"><p>This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.</p> </div> </div> Thu, 11 Mar 2021 21:22:04 +0000 ctran 12831 at https://darkmatter.ucsf.edu