Over Expression Cells for CACNB4

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

CACNB4

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-CACNB4-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Single Cell Clone: B3

Localization: Membrane+Cytosol+Nucleus

Sequence

ORF Sequence:

ATGTCCTCCTCCTCCTACGCCAAGAACGGGACCGCGGACGGGCCGCACTCCCCCACCTCGCAGGTGGCCCGAGGCACCAC AACCCGGAGGAGCAGGTTGAAAAGATCCGATGGCAGCACCACTTCGACCAGCTTCATCCTCAGACAGGGTTCAGCGGATT CCTACACAAGCAGGCCGTCTGACTCCGATGTCTCTTTGGAAGAGGACCGGGAAGCAATTCGACAGGAGAGAGAACAGCAA GCAGCTATCCAGCTTGAGAGAGCAAAGTCCAAACCTGTAGCATTTGCCGTGAAGACAAATGTGAGCTACTGCGGCGCCCT GGACGAGGATGTGCCTGTTCCAAGCACAGCTATCTCCTTTGATGCTAAAGACTTTCTACATATTAAAGAGAAATATAACA ATGATTGGTGGATAGGAAGGCTGGTGAAAGAGGGCTGTGAAATTGGCTTCATTCCAAGTCCACTCAGATTGGAGAACATA CGGATCCAGCAAGAACAAAAAAGAGGACGTTTTCACGGAGGGAAATCAAGTGGAAATTCTTCTTCAAGTCTTGGAGAAAT GGTATCTGGGACATTCCGAGCAACTCCCACATCAACAGCAAAACAGAAGCAAAAAGTGACGGAGCACATTCCTCCTTACG ATGTTGTACCGTCAATGCGTCCGGTGGTGTTAGTGGGGCCGTCACTGAAAGGTTACGAGGTAACAGACATGATGCAGAAA GCCCTCTTTGATTTCCTGAAGCACAGGTTTGATGGGAGGATTTCAATAACGAGAGTGACAGCTGACATTTCTCTTGCTAA GAGGTCTGTCCTAAATAATCCCAGCAAGAGAGCAATAATTGAACGTTCGAACACCCGGTCCAGCTTAGCGGAAGTACAAA GTGAAATTGAAAGAATCTTTGAGTTGGCAAGATCTTTGCAACTGGTTGTTCTTGATGCAGACACCATCAATCACCCAGCA CAACTTATAAAGACTTCCTTAGCACCAATTATTGTTCATGTAAAAGTCTCATCTCCAAAGGTTTTACAGCGGTTGATTAA ATCTAGAGGAAAGTCACAAAGTAAACACTTGAATGTTCAACTGGTGGCAGCTGATAAACTTGCACAATGCCCCCCAGAAA TGTTTGATGTTATATTGGATGAAAATCAGCTTGAGGATGCATGTGAACATCTAGGGGAGTACCTGGAGGCGTACTGGCGT GCCACCCACACAACCAGTAGCACACCCATGACCCCGCTGCTGGGAAGGAATTTGGGCTCCACGGCACTCTCACCATATCC CACAGCAATTTCTGGGTTACAGAGTCAGCGAATGAGGCACAGCAACCACTCCACAGAGAACTCTCCAATTGAAAGACGAA GTCTAATGACCTCTGATGAAAATTATCACAATGAAAGGGCTCGGAAGAGTAGGAACCGCTTGTCTTCCAGTTCTCAGCAT AGCCGAGATCATTACCCTCTTGTGGAAGAAGATTACCCTGACTCATACCAGGACACTTACAAACCCCATAGGAACCGAGG ATCACCTGGGGGATATAGCCATGACTCCCGACATAGGCTTTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCC CTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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