Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGAGTCACTGCAGCAGCCGCGCCCTGACCCTGCTGAGCAGCGTGTTTGGTGCGTGTGGCCTGCTCCTGGTAGGCATCGC
GGTCAGCACTGACTACTGGCTGTACATGGAAGAAGGCACAGTGCTACCGCAGAACCAGACCACCGAGGTCAAGATGGCCC
TGCACGCCGGCCTCTGGCGAGTCTGCTTCTTTGCAGGTCGGGAGAAAGGTCGCTGTGTGGCCTCAGAATATTTTCTTGAA
CCGGAGATCAATTTGGTGACGGAAAACACGGAGAATATTCTGAAGACAGTGCGCACGGCCACCCCCTTCCCCATGGTCAG
CCTCTTCCTCGTGTTCACGGCCTTCGTCATCAGCAACATCGGCCACATCCGCCCGCAGAGGACCATTCTGGCTTTTGTCT
CTGGCATCTTCTTCATACTATCGGGCCTCTCCTTGGTGGTGGGCTTGGTTCTTTACATCTCCAGCATCAACGACGAGGTC
ATGAACAGGCCCAGCAGCTCTGAGCAGTATTTTCATTATCGCTACGGGTGGTCTTTTGCCTTCGCCGCTTCCTCCTTCCT
ACTCAAAGAGGGGGCCGGCGTGATGTCCGTGTACCTGTTCACCAAGCGCTACGCGGAGGAGGAGATGTACCGTCCACACC
CGGCCTTCTACCGCCCGCGTCTCAGCGACTGCTCCGACTACTCGGGCCAGTTCCTGCAGCCCGAGGCGTGGCGCCGCGGC
CGGAGCCCCTCCGACATCTCCAGCGACGTGTCCATCCAAATGACGCAGAACTACCCTCCCGCCATCAAGTACCCGGACCA
CCTGCACATCTCCACCTCGCCCTGCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCG
GTCTCGATTCTACGTAG