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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-GABRP-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGAACTACAGCCTCCACTTGGCCTTCGTGTGTCTGAGTCTCTTCACTGAGAGGATGTGCATCCAGGGGAGTCAGTTCAA
CGTCGAGGTCGGCAGAAGTGACAAGCTTTCCCTGCCTGGCTTTGAGAACCTCACAGCAGGATATAACAAATTTCTCAGGC
CCAATTTTGGTGGAGAACCCGTACAGATAGCGCTGACTCTGGACATTGCAAGTATCTCTAGCATTTCAGAGAGTAACATG
GACTACACAGCCACCATATACCTCCGACAGCGCTGGATGGACCAGCGGCTGGTGTTTGAAGGCAACAAGAGCTTCACTCT
GGATGCCCGCCTCGTGGAGTTCCTCTGGGTGCCAGATACTTACATTGTGGAGTCCAAGAAGTCCTTCCTCCATGAAGTCA
CTGTGGGAAACAGGCTCATCCGCCTCTTCTCCAATGGCACGGTCCTGTATGCCCTCAGAATCACGACAACTGTTGCATGT
AACATGGATCTGTCTAAATACCCCATGGACACACAGACATGCAAGTTGCAGCTGGAAAGCTGGGGCTATGATGGAAATGA
TGTGGAGTTCACCTGGCTGAGAGGGAACGACTCTGTGCGTGGACTGGAACACCTGCGGCTTGCTCAGTACACCATAGAGC
GGTATTTCACCTTAGTCACCAGATCGCAGCAGGAGACAGGAAATTACACTAGATTGGTCTTACAGTTTGAGCTTCGGAGG
AATGTTCTGTATTTCATTTTGGAAACCTACGTTCCTTCCACTTTCCTGGTGGTGTTGTCCTGGGTTTCATTTTGGATCTC
TCTCGATTCAGTCCCTGCAAGAACCTGCATTGGAGTGACGACCGTGTTATCAATGACCACACTGATGATCGGGTCCCGCA
CTTCTCTTCCCAACACCAACTGCTTCATCAAGGCCATCGATGTGTACCTGGGGATCTGCTTTAGCTTTGTGTTTGGGGCC
TTGCTAGAATATGCAGTTGCTCACTACAGTTCCTTACAGCAGATGGCAGCCAAAGATAGGGGGACAACAAAGGAAGTAGA
AGAAGTCAGTATTACTAATATCATCAACAGCTCCATCTCCAGCTTTAAACGGAAGATCAGCTTTGCCAGCATTGAAATTT
CCAGCGACAACGTTGACTACAGTGACTTGACAATGAAAACCAGCGACAAGTTCAAGTTTGTCTTCCGAGAAAAGATGGGC
AGGATTGTTGATTATTTCACAATTCAAAACCCCAGTAATGTTGATCACTATTCCAAACTACTGTTTCCTTTGATTTTTAT
GCTAGCCAATGTATTTTACTGGGCATACTACATGTATTTTACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTA
ACCCTCTCCTCGGTCTCGATTCTACGTAG