Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGGAAGGAGGGTGGCCTGCAAGGCAGAGTGCCCTCCTCTGCCTCACTGTCAGTCTTCTGCTTCAAGGAAGAGGCGACGC
TTTTACCATCAATTGCTCAGGCTTTGACCAGCATGGGGTTGACCCTGCTGTCTTCCAAGCAGTGTTTGACAGAAAGGCCT
TCCGTCCATTCACCAACTACAGCATCCCTACCCGTGTCAACATCTCCTTCACCCTGTCTGCCATCCTGGGAGTGGATGCA
CAGCTCCAGCTGCTGACATCATTCCTGTGGATGGATTTGGTATGGGACAATCCTTTCATTAATTGGAACCCAAAAGAGTG
TGTTGGCATCAATAAACTCACAGTATTAGCTGAAAACCTGTGGCTCCCAGACATCTTCATCGTGGAATCCATGGATGTGG
ATCAGACGCCTTCCGGTCTCACTGCCTATATCAGCAGTGAAGGTCGAATTAAGTATGATAAGCCAATGAGGGTGACCAGC
ATCTGTAACCTGGACATCTTCTACTTCCCTTTTGACCAACAGAACTGTACCTTCACCTTCAGTTCTTTCCTCTACACAGT
GGACAGCATGCTGCTGGGCATGGACAAGGAGGTGTGGGAGATCACAGACACGTCTCGCAAAGTCATCCAAACCCAGGGGG
AGTGGGAGCTCTTGGGCATCAACAAGGCCACCCCAAAGATGTCCATGGGCAACAACCTATATGACCAGATCATGTTTTAT
GTGGCCATCAGGCGCAGGCCAAGCCTCTACATCATAAACCTGCTGGTGCCCAGTAGCTTTCTGGTTGCCATTGATGCCCT
CAGCTTCTACCTGCCAGCAGAGAGCGAGAATCGTGCCCCATTCAAGATAACACTTCTGCTGGGCTACAACGTCTTCCTGC
TCATGATGAATGACTTGCTCCCTGCCAGTGGCACCCCCCTCATCAGTGTCTACTTCGCCCTGTGCCTGTCCCTGATGGTG
GTCAGCCTGCTGGAGACCGTCTTCATTACCTACCTGCTGCACGTGGCCACCACCCAGCCCCCACCCATGCCTAGGTGGCT
TCACTCCCTGCTGCTCCACTGCACCAGCCCAGGGAGATGCTGTCCCACTGCGCCCCAGAAGGGAAATAAGGGCCTGGGTC
TCACCCTCACCCACCTGCCTGGCCCAAAGGAGCCGGGGGAGTTAGCAGGGAAGAAGCTGGGACCCAGAGAGACCGAGCCA
GATGGGGGCTCAGGATGGACAAAGACCCAGCTAATGGAGCTGTGGGTGCAGTTCAGCCACGCGATGGACACCCTGCTCTT
CCGCCTCTACCTGCTCTTCATGGCCTCCTCCATCCTTACTGTCATTGTCCTCTGGAACACCTTGCCAACTTTCTTGTACA
AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
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