Over Expressed Cell Line for KCNG4

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

KCNG4

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-KCNG4-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCCCATGCCTTCCAGAGACGGGGGCCTGCATCCCAGACACCACCACTATGGTTCCCACAGCCCTTGGAGTCAGCTCCT
GTCCAGCCCCATGGAGACGCCGTCCATCAAGGGCCTTTACTACCGGAGGGTGCGGAAGGTGGGTGCCCTGGACGCCTCCC
CAGTGGACCTGAAGAAGGAGATCCTGATCAACGTGGGGGGCAGGAGGTATCTCCTCCCCTGGAGCACACTGGACCGGTTC
CCGCTGAGCCGCCTGAGCAAACTCAGGCTCTGTCGGAGCTACGAGGAGATCGTGCAGCTCTGCGATGATTACGACGAGGA
CAGCCAGGAGTTCTTCTTCGACAGGAGCCCCAGCGCCTTCGGGGTGATCGTGAGCTTCCTGGCGGCCGGGAAGCTGGTGC
TTCTGCAGGAGATGTGCGCGCTGTCCTTCCAGGAGGAGCTGGCCTACTGGGGCATCGAGGAGGCCCACCTGGAGAGGTGC
TGCCTGCGGAAGCTGCTGAGGAAGCTGGAGGAGCTGGAGGAGCTGGCCAAGCTGCACAGGGAGGACGTACTGAGGCAGCA
GAGGGAGACCCGCCGCCCCGCCTCGCACTCCTCGCGCTGGGGCCTGTGCATGAACCGGCTGCGCGAGATGGTGGAAAACC
CGCAGTCCGGGCTGCCCGGGAAGGTCTTCGCTTGCCTCTCCATCCTCTTCGTGGCCACCACAGCCGTCAGCCTGTGTGTC
AGCACCATGCCCGACCTCAGGGCAGAGGAGGACCAGGTGAGCGGCCTCTGCCCAACTTTCTTGTACAAAGTGGTTGGTAA
GCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Over Expressed Cell Line for KCNG4

Basic Information

Sequence

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