Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single Cell Clones:
F8-- Heterogenous expression of V5 across few positive cells.
Sequence
ATGGATGCCATTCACATCGGCATGTCCAGCACCCCCCTGGTGAAGCACACTGCTGGGGCTGGGCTCAAGGCCAACAGACC CCGCGTCATGTCCAAGAGTGGGCACAGCAACGTGAGAATTGACAAAGTGGATGGCATATACCTACTCTACCTGCAAGACC TGTGGACCACAGTTATCGACATGAAGTGGAGATACAAACTCACCCTGTTCGCTGCCACTTTTGTGATGACCTGGTTCCTT TTTGGAGTCATCTACTATGCCATCGCGTTTATTCATGGGGACTTAGAACCCGGTGAGCCCATTTCAAATCATACCCCCTG CATCATGAAAGTGGACTCTCTCACTGGGGCGTTTCTCTTTTCCCTGGAATCCCAGACAACCATTGGCTATGGAGTCCGTT CCATCACAGAGGAATGTCCTCATGCCATCTTCCTGTTGGTTGCTCAGTTGGTCATCACGACCTTGATTGAGATCTTCATC ACCGGAACCTTCCTGGCCAAAATCGCCAGACCCAAAAAGCGGGCTGAGACCATCAAGTTCAGCCACTGTGCAGTCATCAC CAAGCAGAATGGGAAGCTGTGCTTGGTGATTCAGGTAGCCAATATGAGGAAGAGCCTCTTGATTCAGTGCCAGCTCTCTG GCAAGCTCCTGCAGACCCACGTCACCAAGGAGGGGGAGCGGATTCTCCTCAACCAAGCCACTGTCAAATTCCACGTGGAC TCCTCCTCTGAGAGCCCCTTCCTCATTCTGCCCATGACATTCTACCATGTGCTGGATGAGACGAGCCCCCTGAGAGACCT CACACCCCAAAACCTAAAGGAGAAGGAGTTTGAGCTTGTGGTCCTCCTCAATGCCACTGTGGAATCCACCAGCGCTGTCT GCCAGAGCCGAACATCTTATATCCCAGAGGAAATCTACTGGGGTTTTGAGTTTGTGCCTGTGGTATCTCTCTCCAAAAAT GGAAAATATGTGGCTGATTTCAGTCAGTTTGAACAGATTCGGAAAAGCCCAGATTGCACATTTTACTGTGCAGATTCTGA GAAACAGCAACTCGAGGAGAAGTACAGGCAGGAGGATCAGAGGGAAAGAGAACTGAGGACACTTTTATTACAACAGAGCA ATGTCTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
