Over Expressed Cell Line for KCNK7

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

KCNK7

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-KCNK7-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGGGGGTCTAAGGCCCTGGTCCCGATACGGGCTCCTGGTTGTGGCCCACTTGCTGGCCCTGGGGCTTGGGGCTGTGGT
GTTCCAGGCCCTGGAGGGGCCTCCTGCATGCAGGCTTCAGGCTGAGCTCAGGGCAGAGCTGGCAGCCTTCCAGGCAGAGC
ATAGGGCCTGCCTGCCACCCGGAGCTCTGGAAGAGCTGCTGGGCACTGCCCTGGCCACCCAGGCCCATGGGGTCTCCACC
CTGGGCAACAGCTCAGAGGGCAGGACCTGGGACCTTCCCTCAGCCCTGCTCTTCGCTGCCAGCATCCTCACCACCACAGG
TTATGGCCACATGGCCCCACTATCGCCAGGCGGAAAGGCCTTCTGCATGGTCTATGCAGCCCTGGGGCTGCCAGCCTCCT
TAGCTCTCGTGGCCACCCTGCGCCATTGCCTGCTGCCTGTGCTCAGCCGCCCACGTGCCTGGGTAGCGGTCCACTGGCAG
CTGTCACCGGCCAGGGCTGCGCTGCTGCAGGCAGTTGCACTGGGACTGCTGGTGGCCAGCAGCTTTGTGCTGCTGCCAGC
GCTGGTGCTGTGGGGCCTTCAGGGCGACTGCAGCCTGCTGGGGGCCGTCTACTTCTGCTTCAGCTCGCTCAGCACCATTG
GCCTGGAGGACTTGCTGCCCGGCCGCGGCCGCAGCCTGCACCCCGTGATTTACCACCTGGGCCAGCTCGCACTTCTTGGT
GGAGGGACCTCACTCCAGGGCACGGCGTGGGAGGGGACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGTAG

Over Expressed Cell Line for KCNK7

Basic Information

Sequence

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