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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-PKD2L2-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGCTGAGGCGTCACGGTGGCACCGAGGCGGGGCTTCGAAACATAAGTTGCATTACAGAAAGGAAGTAGAAATTACAAC
CACACTTCAGGAATTGTTACTCTACTTTATTTTTTTAATAAACCTATGTATATTGACTTTTGGGATGGTAAACCCACATA
TGTATTACTTAAACAAGGTTATGTCATCTCTATTTTTGGACACTTCTGTGCCTGGTGAAGAAAGAACCAACTTTAAGTCC
ATTCGCAGCATAACTGATTTTTGGAAGTTTATGGAAGGACCCCTTTTGGAAGGTCTGTACTGGGATTCATGGTACAATAA
CCAGCAGCTGTATAATTTAAAGAACAGCAGTCGCATCTACTATGAAAATATACTTCTAGGAGTTCCCAGAGTTCGTCAAC
TAAAAGTCCGCAACAACACATGCAAAGTCTATTCATCTTTTCAGTCTTTGATGAGTGAATGTTATGGCAAATATACTTCT
GCAAATGAAGACCTCTCTAATTTTGGCCTTCAAATTAATACTGAATGGAGATATTCTACTTCTAATACCAACTCCCCTTG
GCACTGGGGATTTCTTGGTGTTTACCGAAATGGGGGATACATTTTCACTTTATCAAAATCGAAATCTGAAACCAAAAACA
AGTTCATTGACCTTCGACTGAACAGCTGGATCACAAGAGGGACTAGAGTTATTTTTATTGATTTTTCCTTATATAATGCT
AATGTAAATCTATTTTGTATTATCAGATTGGTGGCAGAATTCCCTGCAACTGGAGGAATACTTACTTCATGGCAGTTTTA
CTCTGTGAAGCTCCTCAGATATGTTAGCTACTATGACTATTTTATTGCTTCCTGTGAAATCACATTCTGTATTTTTCTTT
TTGTCTTCACAACACAAGAAGTCAAAAAAATAAAAGAATTTAAGTCTGCCTATTTCAAAAGTATTTGGAACTGGCTAGAA
TTGCTACTTTTGCTGTTGTGTTTTGTGGCTGTTTCCTTCAACACATACTATAATGTACAAATTTTTCTCTTACTTGGACA
GCTGTTGAAAAGTACTGAAAAATATTCAGATTTCTATTTTCTTGCATGCTGGCACATTTATTACAATAATATAATTGCTA
TTACCATCTTTTTTGCATGGATAAAGAATATGTTCTTGGCAATTATTAATGATACCTATTCTGAAGTGAAAGCTGACTAT
TCAATAGGCAGAAGGCCAGATTTTGAACTTGGCAAAATGATTAAACAGAGTTACAAAAATGTTCTCGAGAAATTCAGACT
GAAGAAAGCTCAAAAAGATGAAGACAAGAAAACCAAAGGCAGCGGAGATTTGGCTGAACAAGCCAGAAGAGAAGGCTTTG
ACGAAAATGAGATTCAAAACGCAGAGCAGATGAAAAAATGGAAAGAGAGGCTTGAGAAAAAGTATTATTCTATGGAAATT
CAAGATGACTACCAGCCTGTCACTCAAGAAGAATTTCGAGAACTTTTTTTATATGCTGTGGAGCTGGAGAAGGAATTACA
CTACATCAATTTGAAGCTAAATCAAGTGGTGAGAAAGGTTTCAGCTCTATACCCAACTTTCTTGTACAAAGTGGTTGGTA
AGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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