Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGTCTGCCTCACCAGACGACCTGAGTACAGGGGGAAGGTTACAGAACATGACAGTGGATGAATGCTTCCAGTCTCGGAA
CACCGTCCTCCAGGGGCAGCCCTTTGGGGGTGTCCCCACCGTGCTGTGCCTCAACATCGCCCTGTGGGTGCTCGTCCTTG
TGGTTTACTCCTTCCTCCGGAAAGCTGCGTGGGACTATGGGCGCCTGGCTCTGCTGATACACAATGACAGCCTGACCTCG
CTGATCTATGGGGAGCAGAGCGAGAAGACATCTCCCTCGGAGACTTCCTTGGAGATGGAACGCAGAGACAAGGGATTCTG
TTCCTGGTTCTTCAACAGCATAACAATGAAGGACGAGGATCTGATTAACAAGTGTGGGGACGACGCGCGCATCTACATCG
TGTTCCAGTACCACCTCATCATCTTTGTGCTCATCATCTGTATCCCCTCCCTGGGCATCATTTTGCCCATCAACTATACT
GGATCTGTTCTGGACTGGAGCAGTCACTTTGCTCGGACCACCATTGTCAATGTCTCCACAGAGAGCAAGCTCCTGTGGCT
GCATAGCCTGCTGTCCTTCTTCTACTTCATCACCAACTTCATGTTCATGGCTCATCACTGCCTGGGGTTTGCGCCCAGGA
ATAGCCAAAAGGTCACAAGGACACTAATGATCACCTATGTGCCCAAGGACATTGAAGACCCAGAACTCATCATTAAGCAT
TTTCACGAGGCCTATCCAGGCAGTGTCGTGACAAGAGTCCACTTCTGCTACGACGTCAGGAACCTGATCGACTTGGACGA
TCAGAGGCGCCATGCCATGCGGGGCCGGCTTTTCTATACAGCCAAGGCCAAGAAGACTGGGAAGGTGATGATCAGGATCC
ACCCCTGTGCCCGCCTGTGCTTCTGCAAGTGCTGGACCTGCTTCAAGGAGGTGGATGCAGAGCAGTATTACAGCGAGCTA
GAGGAGCAGCTAACGGACGAGTTCAACGCCGAGCTCAACCGCGTGCCGCTCAAGCGGCTGGACCTGATCTTTGTCACCTT
CCAGGACTCCAGGATGGCCAAGCGTGTCCGTAAGGATTACAAGTATGTCCAGTGTGGTGTGCAACCCCAGCAGTCCTCAG
TGACCACCATCGTCAAATCATATTACTGGAGGGTCACTATGGCCCCACACCCCAAAGACATTATTTGGAAACACCTGTCT
GTCCGCCGCTTCTTTTGGTGGGCCCGCTTTATCGCAATCAACACCTTCCTCTTCTTCCTCTTCTTCTTTCTCACCACGCC
TGCCATCATCATGAACACTATCGACATGTACAACGTCACCCGCCCCATCGAGAAGCTGCAGAACCCAATTGTGACCCAGT
TCTTCCCCTCTGTGATGCTCTGGGGCTTCACAGTGATACTGCCTCTGATTGTCTACTTCTCCGCCTTCCTCGAGGCCCAC
TGGACCAGATCAAGTCAGAATCTGGTCATGGTGCACAAGTGCTACATCTTTCTGGTGTTCATGGTAGTCATTCTGCCCTC
TATGGGACTGACCAGTTTGGATGTCTTTCTCCGCTGGCTCTTTGACATCTACTATCTAGAGCAAGCATCCATCAGGTTCC
AGTGTGTGTTCCTGCCAGACAACGGCGCCTTCTTTGTCAACTACGTGATCACGGCAGCTTTACTTGGCACAGGCATGGAG
CTGCTGCGTCTGGGGTCACTCTTCTGCTACAGCACCCGCCTCTTCTTCTCTAGATCAGAGCCAGAGAGAGTCAACATCAG
AAAGAACCAGGCCATAGACTTCCAGTTTGGGCGTGAGTATGCGTGGATGATGAACGTGTTCAGCGTGGTGATGGCGTACA
GCATCACTTGCCCCATCATTGTGCCTTTTGGGTTGCTCTACCTGTGCATGAAGCACTTGACGGATCGCTATAACATGTAC
TACTCCTTTGCACCCACCAAACTGAACGAGCAGATCCACATGGCTGCCGTCTCCCAGGCCATCTTTGCGCCACTCTTGGG
TCTGTTCTGGATGCTGTTCTTCTCCATCCTGCGGTTGGGTTCTCTCCACGCCATCACCATCTTTTCCCTGTCCACCCTCC
TCATTGCCATGGTGATTGCCTTTGTTGGCATTTTTCTGGGGAAGCTTCGGATGGTTGCCGACTACGAGCCCGAGGAGGAG
GAGATCCAGACAGTGTTTGACATGGAGCCAAGCAGCACCTCCTCCACGCCCACCTCCCTCCTGTATGTGGCCACCGTGCT
GCAAGAACCGGAGTTGAATCTGACCCCCGCCTCCTCCCCAGCCAGGCACACCTATGGCACCATGAACAACCAGCCGGAAG
AGGGAGAAGAAGAGAGTGGTCTGAGGGGCTTTGCGAGGGAGCTAGACTCGGCCCAGTTCCAGGAAGGGCTGGAACTGGAG
GGCCAGAACCAGTACCACTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGA
TTCTACGTAG
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