Over Expression Cells for CACNG1

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

CACNG1

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-CACNG1-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Single-Cell Clones:

A4 A6 A11

A7 Possibly clonal

V5 Expression is membranous. Expression between clones looks homogenous. Cells appear to be performing exocytosis (see punta).

Sequence

ORF Sequence:

ATGTCCCAGACCAAAATGCTGAAGGTCCGCGTGACCCTCTTCTGCATCCTGGCAGGCATCGTGCTGGCCATGACAGCCGT GGTAACCGACCACTGGGCTGTGCTGAGCCCCCACATGGAGCACCACAACACTACCTGCGAGGCGGCCCACTTCGGCCTCT GGCGGATTTGTACCAAGCGCATCCCCATGGACGACAGCAAGACCTGCGGGCCCATCACCCTGCCCGGGGAGAAGAACTGT TCCTACTTCAGGCATTTTAACCCCGGCGAGAGCTCGGAGATCTTCGAATTCACCACTCAGAAGGAGTACAGCATCTCGGC AGCCGCCATCGCCATCTTCAGCCTTGGCTTCATCATCCTGGGCAGCCTCTGTGTCCTCCTGTCCCTCGGGAAGAAGAGGG ACTATCTGCTGCGACCCGCGTCCATGTTCTATGCCTTTGCAGGTCTCTGCATCCTCGTCTCGGTGGAGGTCATGCGGCAG TCGGTGAAGCGCATGATTGACAGTGAGGACACCGTCTGGATCGAGTACTATTACTCCTGGTCCTTTGCCTGCGCCTGTGC CGCCTTCATCCTCCTCTTTCTCGGCGGTCTCGCCCTCCTGCTGTTCTCCCTGCCTCGAATGCCCCGGAACCCATGGGAGT CCTGCATGGATGCTGAGCCCGAGCACTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTC GGTCTCGATTCTACGTAG

Media and Files