This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single-Cell Clones:
A4 A6 A11
A7 Possibly clonal
V5 Expression is membranous. Expression between clones looks homogenous. Cells appear to be performing exocytosis (see punta).