Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single Cell Clones:
There are 5 single cell clonal lines. D1, D2, D7, D8, E1. V5 expression in cytosol and membrane. V5 Expression is higher in the membrane.
Sequence
ATGACCATCGCCATCAGCACTGACTACTGGCTCTACACGCGCGCCCTCATCTGCAACACCACCAACCTCACGGCCGGCGG CGACGACGGGACCCCCCACCGCGGGGGCGGCGGCGCCTCGGAGAAGAAGGACCCCGGCGGCCTCACGCACTCGGGCCTCT GGAGGATCTGCTGCCTGGAAGGGTTGAAAAGAGGCGTCTGCGTGAAGATCAATCATTTCCCGGAGGACACGGACTACGAC CACGACAGCGCGGAGTATCTACTCCGAGTTGTCCGGGCCTCCAGCATCTTCCCCATCCTTAGCGCCATCCTGCTGCTGCT CGGGGGTGTGTGCGTGGCGGCCTCCCGCGTCTACAAGTCCAAGAGGAACATCATTCTGGGCGCAGGGATCCTGTTCGTGG CAGCAGGCCTGAGCAACATCATCGGCGTGATCGTGTACATCTCCGCCAACGCGGGCGAGCCGGGCCCGAAGCGGGACGAG GAGAAGAAAAACCACTACTCGTACGGCTGGTCCTTCTACTTCGGCGGGCTGTCGTTCATCCTGGCCGAGGTGATAGGCGT GCTGGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCGCACTGCCAGTCTCGCTCGGACCTGCTCAAGGCCGGCGGGG GCGCGGGCGGCAGTGGCGGGAGCGGCCCCTCGGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCC CGCTCTAGCTCCCGCTCCAGCGAGCCGTCGCCGTCGCGGGACGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTTGCCTC CACGGACATCTCCATGTACACGCTCAGCCGCGACCCCTCCAAGGGCAGCGTGGCCGCGGGGCTGGCGGGGGCCGGCGGCG GCGgcggcggcgccgtgggggcgttcGGCGGCGCGGCCGGGGGCGCCGGGGGCGGCGGCGGAGGCGGCGGCGGGGCGGGT GCCGAGCGGGACCGCGGGGGGGCGTCCGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGGCGGCGGCGTCAC GGTCACGGTCACCGGGCCGcccGCCCCGCCCGCGCCCGCGCCACCCGCGCCCTCTGCGCCCGCCCCCGGGACCCTGGCCA AGGAGGCCGCCGCCTCCAACACCAACACGCTCAACAGGAAAACCACGCCTGTGTCAGGCCTCATGGGCCCAGCTTTCTTG TACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
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