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Data and Resources for CACNG8 Gene

Disease Associated Data

Basic Information

Gene Taxonomy:
CACNG8
UniprotID:
Q8WXS5
Mouse Homolog:
Cacng8
Pharos Link:
Pharos Link
Knockout Mouse Phenotype:
nervous system phenotype
Abnormal synaptic transmission
Abnormality of reproductive system physiology
abnormal excitatory postsynaptic currents
abnormal glutamate-mediated receptor currents
abnormal miniature excitatory postsynaptic currents
reduced long term potentiation
abnormal hippocampus pyramidal cell morphology
abnormal AMPA-mediated synaptic currents
Mortality/Aging
Monogenic Disease Association:
Not Detected
Polygenic Disease Association:
schizophrenia
10K Immunome:
Not Detected
SARS-CoV-2 Association:
Not Detected
SARS-CoV-2 Vaccine Association:
Not Detected

Over Expression Cell Lines

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
CACNG8
Cell Line Taxonomy:
293T
Over Expression Cell Line
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CACNG8-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

 

Single Cell Clones: 
There are 5 single cell clonal lines. D1, D2, D7, D8, E1. V5 expression in cytosol and membrane. V5 Expression is higher in the membrane.

Sequence

ORF Sequence:

ATGACCATCGCCATCAGCACTGACTACTGGCTCTACACGCGCGCCCTCATCTGCAACACCACCAACCTCACGGCCGGCGG CGACGACGGGACCCCCCACCGCGGGGGCGGCGGCGCCTCGGAGAAGAAGGACCCCGGCGGCCTCACGCACTCGGGCCTCT GGAGGATCTGCTGCCTGGAAGGGTTGAAAAGAGGCGTCTGCGTGAAGATCAATCATTTCCCGGAGGACACGGACTACGAC CACGACAGCGCGGAGTATCTACTCCGAGTTGTCCGGGCCTCCAGCATCTTCCCCATCCTTAGCGCCATCCTGCTGCTGCT CGGGGGTGTGTGCGTGGCGGCCTCCCGCGTCTACAAGTCCAAGAGGAACATCATTCTGGGCGCAGGGATCCTGTTCGTGG CAGCAGGCCTGAGCAACATCATCGGCGTGATCGTGTACATCTCCGCCAACGCGGGCGAGCCGGGCCCGAAGCGGGACGAG GAGAAGAAAAACCACTACTCGTACGGCTGGTCCTTCTACTTCGGCGGGCTGTCGTTCATCCTGGCCGAGGTGATAGGCGT GCTGGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCGCACTGCCAGTCTCGCTCGGACCTGCTCAAGGCCGGCGGGG GCGCGGGCGGCAGTGGCGGGAGCGGCCCCTCGGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCC CGCTCTAGCTCCCGCTCCAGCGAGCCGTCGCCGTCGCGGGACGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTTGCCTC CACGGACATCTCCATGTACACGCTCAGCCGCGACCCCTCCAAGGGCAGCGTGGCCGCGGGGCTGGCGGGGGCCGGCGGCG GCGgcggcggcgccgtgggggcgttcGGCGGCGCGGCCGGGGGCGCCGGGGGCGGCGGCGGAGGCGGCGGCGGGGCGGGT GCCGAGCGGGACCGCGGGGGGGCGTCCGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGGCGGCGGCGTCAC GGTCACGGTCACCGGGCCGcccGCCCCGCCCGCGCCCGCGCCACCCGCGCCCTCTGCGCCCGCCCCCGGGACCCTGGCCA AGGAGGCCGCCGCCTCCAACACCAACACGCTCAACAGGAAAACCACGCCTGTGTCAGGCCTCATGGGCCCAGCTTTCTTG TACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

CACNG8 D8
CACNG8 D2

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:
CACNG8
Virus Type:
Lentiviral Vector
Vector Type:
cDNA
Construct ID:
IDG_CACNG8_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGACCATCGCCATCAGCACTGACTACTGGCTCTACACGCGCGCCCTCATCTGCAACACCACCAACCTCACGGCCGGCGG
CGACGACGGGACCCCCCACCGCGGGGGCGGCGGCGCCTCGGAGAAGAAGGACCCCGGCGGCCTCACGCACTCGGGCCTCT
GGAGGATCTGCTGCCTGGAAGGGTTGAAAAGAGGCGTCTGCGTGAAGATCAATCATTTCCCGGAGGACACGGACTACGAC
CACGACAGCGCGGAGTATCTACTCCGAGTTGTCCGGGCCTCCAGCATCTTCCCCATCCTTAGCGCCATCCTGCTGCTGCT
CGGGGGTGTGTGCGTGGCGGCCTCCCGCGTCTACAAGTCCAAGAGGAACATCATTCTGGGCGCAGGGATCCTGTTCGTGG
CAGCAGGCCTGAGCAACATCATCGGCGTGATCGTGTACATCTCCGCCAACGCGGGCGAGCCGGGCCCGAAGCGGGACGAG
GAGAAGAAAAACCACTACTCGTACGGCTGGTCCTTCTACTTCGGCGGGCTGTCGTTCATCCTGGCCGAGGTGATAGGCGT
GCTGGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCGCACTGCCAGTCTCGCTCGGACCTGCTCAAGGCCGGCGGGG
GCGCGGGCGGCAGTGGCGGGAGCGGCCCCTCGGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCC
CGCTCTAGCTCCCGCTCCAGCGAGCCGTCGCCGTCGCGGGACGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTTGCCTC
CACGGACATCTCCATGTACACGCTCAGCCGCGACCCCTCCAAGGGCAGCGTGGCCGCGGGGCTGGCGGGGGCCGGCGGCG
GCGGCGGCGGCGCCGTGGGGGCGTTCGGCGGCGCGGCCGGGGGCGCCGGGGGCGGCGGCGGAGGCGGCGGCGGGGCGGGT
GCCGAGCGGGACCGCGGGGGGGCGTCCGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGGCGGCGGCGTCAC
GGTCACGGTCACCGGGCCGCCCGCCCCGCCCGCGCCCGCGCCACCCGCGCCCTCTGCGCCCGCCCCCGGGACCCTGGCCA
AGGAGGCCGCCGCCTCCAACACCAACACGCTCAACAGGAAAACCACGCCTGTGTCAGGCCTCATGGGCCCAGCTTTCTTG
TACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:
D.IC87-pLX304-CACNG8-expression-vector.dna

Constructs for CRISPR Interference

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Exploring the Dark Genome