Skip to main content
Loading...

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CACNG8-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

 

Single Cell Clones: 
There are 5 single cell clonal lines. D1, D2, D7, D8, E1. V5 expression in cytosol and membrane. V5 Expression is higher in the membrane.

Sequence

ORF Sequence:

ATGACCATCGCCATCAGCACTGACTACTGGCTCTACACGCGCGCCCTCATCTGCAACACCACCAACCTCACGGCCGGCGG CGACGACGGGACCCCCCACCGCGGGGGCGGCGGCGCCTCGGAGAAGAAGGACCCCGGCGGCCTCACGCACTCGGGCCTCT GGAGGATCTGCTGCCTGGAAGGGTTGAAAAGAGGCGTCTGCGTGAAGATCAATCATTTCCCGGAGGACACGGACTACGAC CACGACAGCGCGGAGTATCTACTCCGAGTTGTCCGGGCCTCCAGCATCTTCCCCATCCTTAGCGCCATCCTGCTGCTGCT CGGGGGTGTGTGCGTGGCGGCCTCCCGCGTCTACAAGTCCAAGAGGAACATCATTCTGGGCGCAGGGATCCTGTTCGTGG CAGCAGGCCTGAGCAACATCATCGGCGTGATCGTGTACATCTCCGCCAACGCGGGCGAGCCGGGCCCGAAGCGGGACGAG GAGAAGAAAAACCACTACTCGTACGGCTGGTCCTTCTACTTCGGCGGGCTGTCGTTCATCCTGGCCGAGGTGATAGGCGT GCTGGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCGCACTGCCAGTCTCGCTCGGACCTGCTCAAGGCCGGCGGGG GCGCGGGCGGCAGTGGCGGGAGCGGCCCCTCGGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCC CGCTCTAGCTCCCGCTCCAGCGAGCCGTCGCCGTCGCGGGACGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTTGCCTC CACGGACATCTCCATGTACACGCTCAGCCGCGACCCCTCCAAGGGCAGCGTGGCCGCGGGGCTGGCGGGGGCCGGCGGCG GCGgcggcggcgccgtgggggcgttcGGCGGCGCGGCCGGGGGCGCCGGGGGCGGCGGCGGAGGCGGCGGCGGGGCGGGT GCCGAGCGGGACCGCGGGGGGGCGTCCGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGGCGGCGGCGTCAC GGTCACGGTCACCGGGCCGcccGCCCCGCCCGCGCCCGCGCCACCCGCGCCCTCTGCGCCCGCCCCCGGGACCCTGGCCA AGGAGGCCGCCGCCTCCAACACCAACACGCTCAACAGGAAAACCACGCCTGTGTCAGGCCTCATGGGCCCAGCTTTCTTG TACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_CACNG8_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGACCATCGCCATCAGCACTGACTACTGGCTCTACACGCGCGCCCTCATCTGCAACACCACCAACCTCACGGCCGGCGG
CGACGACGGGACCCCCCACCGCGGGGGCGGCGGCGCCTCGGAGAAGAAGGACCCCGGCGGCCTCACGCACTCGGGCCTCT
GGAGGATCTGCTGCCTGGAAGGGTTGAAAAGAGGCGTCTGCGTGAAGATCAATCATTTCCCGGAGGACACGGACTACGAC
CACGACAGCGCGGAGTATCTACTCCGAGTTGTCCGGGCCTCCAGCATCTTCCCCATCCTTAGCGCCATCCTGCTGCTGCT
CGGGGGTGTGTGCGTGGCGGCCTCCCGCGTCTACAAGTCCAAGAGGAACATCATTCTGGGCGCAGGGATCCTGTTCGTGG
CAGCAGGCCTGAGCAACATCATCGGCGTGATCGTGTACATCTCCGCCAACGCGGGCGAGCCGGGCCCGAAGCGGGACGAG
GAGAAGAAAAACCACTACTCGTACGGCTGGTCCTTCTACTTCGGCGGGCTGTCGTTCATCCTGGCCGAGGTGATAGGCGT
GCTGGCCGTCAACATCTACATCGAGCGCAGCCGCGAGGCGCACTGCCAGTCTCGCTCGGACCTGCTCAAGGCCGGCGGGG
GCGCGGGCGGCAGTGGCGGGAGCGGCCCCTCGGCCATCCTCCGTCTGCCCAGTTACCGCTTCCGCTACCGCCGCCGCTCC
CGCTCTAGCTCCCGCTCCAGCGAGCCGTCGCCGTCGCGGGACGCGTCTCCCGGCGGCCCCGGGGGCCCGGGCTTTGCCTC
CACGGACATCTCCATGTACACGCTCAGCCGCGACCCCTCCAAGGGCAGCGTGGCCGCGGGGCTGGCGGGGGCCGGCGGCG
GCGGCGGCGGCGCCGTGGGGGCGTTCGGCGGCGCGGCCGGGGGCGCCGGGGGCGGCGGCGGAGGCGGCGGCGGGGCGGGT
GCCGAGCGGGACCGCGGGGGGGCGTCCGGCTTCCTCACGCTGCACAACGCCTTCCCCAAGGAGGCGGGCGGCGGCGTCAC
GGTCACGGTCACCGGGCCGCCCGCCCCGCCCGCGCCCGCGCCACCCGCGCCCTCTGCGCCCGCCCCCGGGACCCTGGCCA
AGGAGGCCGCCGCCTCCAACACCAACACGCTCAACAGGAAAACCACGCCTGTGTCAGGCCTCATGGGCCCAGCTTTCTTG
TACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files