Over Expression Cells for CLCN6

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

CLCN6

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-CLCN6-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Single cell clone: A10

Subcellular localization: Membrane + intracellular

Sequence

ORF Sequence:

ATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCCGCACCCCCGA GGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGACTATGAGAGTTTGGATTATG ATCGCTGTATCAATGACCCTTACCTGGAAGTTTTGGAGACCATGGATAATAAGAAAGGTCGAAGATATGAGGCGGTGAAG TGGATGGTGGTGTTTGCCATTGGAGTCTGCACTGGCCTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCA ACTCAAGTTCGGAGTGGTACAGACATCGGTGGAGGAGTGCAGCCAGAAAGGCTGCCTCGCTCTGTCTCTCCTTGAACTCC TGGGTTTTAACCTCACCTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGGATACCC GAGGTCAAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTGGAGT GCTGTTCAGTGTGGCTGGAGGGCTCTTCGTGGGGAAGGAAGGCCCCATGATCCACAGTGGTTCGGTGGTGGGAGCTGGCC TCCCTCAGTTTCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTCCCCTATTTCCGAAGCGACAGAGACAAGAGAGAC TTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGGCGCCAATCGGGGGTACCTTGTTCAGTCTAGAGGA GGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAAAGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCAACTTCT TCCATTCTGGGATTCAGTTTGGAAGCTGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGTGCTCT GACTCTGATAAAAAATGTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGGGGCCTCCT GGGAGCCACATTCAACTGTCTGAACAAGAGGCTTGCAAAGTACCGTATGCGAAACGTGCACCCGAAACCTAAGCTCGTCA GAGTCTTAGAGAGCCTCCTTGTGTCTCTGGTAACCACCGTGGTGGTGTTTGTGGCCTCGATGGTGTTAGGAGAATGCCGA CAGATGTCCTCTTCGAGTCAAATCGGTAATGACTCATTCCAGCTCCAGGTCACAGAAGATGTGAATTCAAGTATCAAGAC ATTTTTTTGTCCCAATGATACCTACAATGACATGGCCACACTCTTCTTCAACCCGCAGGAGTCTGCCATCCTCCAGCTCT TCCACCAGGATGGTACTTTCAGCCCCGTCACTCTGGCCTTGTTCTTCGTTCTCTATTTCTTGCTTGCATGTTGGACTTAC GGCATTTCTGTTCCAAGTGGCCTTTTTGTGCCTTCTCTGCTGTGTGGAGCTGCTTTTGGACGTTTAGTTGCCAATGTCCT AAAAAGCTACATTGGATTGGGCCACATCTATTCGGGGACCTTTGCCCTGATTGGTGCAGCGGCTTTCTTGGGCGGGGTGG TCCGCATGACCATCAGCCTCACGGTCATCCTGATCGAGTCCACCAATGAGATCACCTACGGGCTCCCCATCATGGTCACA CTGATGGTGGCCAAATGGACAGGGGACTTTTTCAATAAGGGCATTTATGATATCCACGTGGGCCTGCGAGGCGTGCCGCT TCTGGAATGGGAGACAGAGGTGGAAATGGACAAGCTGAGAGCCAGCGACATCATGGAGCCCAACCTGACCTACGTCTACC CGCACACCCGCATCCAGTCTCTGGTGAGCATCCTGCGCACCACGGTCCACCATGCCTTCCCGGTGGTCACAGAGAACCGC GGTAACGAGAAGGAGTTCATGAAGGGCAACCAGCTCATCAGCAACAACATCAAGTTCAAGAAATCCAGCATCCTCACCCG GGCTGGCGAGCAGCGCAAACGGAGCCAGTCCATGAAGTCCTACCCATCCAGCGAGCTACGGAACATGTGTGATGAGCACA TCGCCTCTGAGGAGCCAGCCGAGAAGGAGGACCTCCTGCAGCAGATGCTGGAAAGGAGATACACTCCCTACCCCAACCTA TACCCTGACCAGTCCCCAAGTGAAGACTGGACCATGGAGGAGCGGTTCCGCCCTCTGACCTTCCACGGCCTGATCCTTCG GTCGCAGCTTGTCACCCTGCTTGTCCGAGGAGTTTGTTACTCTGAAAGCCAGTCGAGCGCCAGCCAGCCGCGCCTCTCCT ATGCCGAGATGGCCGAGGACTACCCGCGGTACCCCGACATCCACGACCTGGACCTGACGCTGCTCAACCCGCGCATGATC GTGGATGTCACCCCATACATGAACCCTTCGCCTTTCACCGTCTCGCCCAACACCCACGTCTCCCAAGTCTTCAACCTGTT CAGAACGATGGGCCTGCGCCACCTGCCCGTGGTGAACGCTGTGGGAGAGATCGTGGGGATCATCACACGGCACAACCTCA CCTATGAATTTCTGCAGGCCCGGCTGAGGCAGCACTACCAGACCATCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAG CCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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