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10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CLCN6-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCCGCACCCCCGA
GGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGACTATGAGAGTTTGGATTATG
ATCGCTGTATCAATGACCCTTACCTGGAAGTTTTGGAGACCATGGATAATAAGAAAGGTCGAAGATATGAGGCGGTGAAG
TGGATGGTGGTGTTTGCCATTGGAGTCTGCACTGGCCTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCA
ACTCAAGTTCGGAGTGGTACAGACATCGGTGGAGGAGTGCAGCCAGAAAGGCTGCCTCGCTCTGTCTCTCCTTGAACTCC
TGGGTTTTAACCTCACCTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGGATACCC
GAGGTCAAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTGGAGT
GCTGTTCAGTGTGGCTGGAGGGCTCTTCGTGGGGAAGGAAGGCCCCATGATCCACAGTGGTTCGGTGGTGGGAGCTGGCC
TCCCTCAGTTTCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTCCCCTATTTCCGAAGCGACAGAGACAAGAGAGAC
TTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGGCGCCAATCGGGGGTACCTTGTTCAGTCTAGAGGA
GGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAAAGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCAACTTCT
TCCATTCTGGGATTCAGTTTGGAAGCTGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGTGCTCT
GACTCTGATAAAAAATGTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGGGGCCTCCT
GGGAGCCACATTCAACTGTCTGAACAAGAGGCTTGCAAAGTACCGTATGCGAAACGTGCACCCGAAACCTAAGCTCGTCA
GAGTCTTAGAGAGCCTCCTTGTGTCTCTGGTAACCACCGTGGTGGTGTTTGTGGCCTCGATGGTGTTAGGAGAATGCCGA
CAGATGTCCTCTTCGAGTCAAATCGGTAATGACTCATTCCAGCTCCAGGTCACAGAAGATGTGAATTCAAGTATCAAGAC
ATTTTTTTGTCCCAATGATACCTACAATGACATGGCCACACTCTTCTTCAACCCGCAGGAGTCTGCCATCCTCCAGCTCT
TCCACCAGGATGGTACTTTCAGCCCCGTCACTCTGGCCTTGTTCTTCGTTCTCTATTTCTTGCTTGCATGTTGGACTTAC
GGCATTTCTGTTCCAAGTGGCCTTTTTGTGCCTTCTCTGCTGTGTGGAGCTGCTTTTGGACGTTTAGTTGCCAATGTCCT
AAAAAGCTACATTGGATTGGGCCACATCTATTCGGGGACCTTTGCCCTGATTGGTGCAGCGGCTTTCTTGGGCGGGGTGG
TCCGCATGACCATCAGCCTCACGGTCATCCTGATCGAGTCCACCAATGAGATCACCTACGGGCTCCCCATCATGGTCACA
CTGATGGTGGCCAAATGGACAGGGGACTTTTTCAATAAGGGCATTTATGATATCCACGTGGGCCTGCGAGGCGTGCCGCT
TCTGGAATGGGAGACAGAGGTGGAAATGGACAAGCTGAGAGCCAGCGACATCATGGAGCCCAACCTGACCTACGTCTACC
CGCACACCCGCATCCAGTCTCTGGTGAGCATCCTGCGCACCACGGTCCACCATGCCTTCCCGGTGGTCACAGAGAACCGC
GGTAACGAGAAGGAGTTCATGAAGGGCAACCAGCTCATCAGCAACAACATCAAGTTCAAGAAATCCAGCATCCTCACCCG
GGCTGGCGAGCAGCGCAAACGGAGCCAGTCCATGAAGTCCTACCCATCCAGCGAGCTACGGAACATGTGTGATGAGCACA
TCGCCTCTGAGGAGCCAGCCGAGAAGGAGGACCTCCTGCAGCAGATGCTGGAAAGGAGATACACTCCCTACCCCAACCTA
TACCCTGACCAGTCCCCAAGTGAAGACTGGACCATGGAGGAGCGGTTCCGCCCTCTGACCTTCCACGGCCTGATCCTTCG
GTCGCAGCTTGTCACCCTGCTTGTCCGAGGAGTTTGTTACTCTGAAAGCCAGTCGAGCGCCAGCCAGCCGCGCCTCTCCT
ATGCCGAGATGGCCGAGGACTACCCGCGGTACCCCGACATCCACGACCTGGACCTGACGCTGCTCAACCCGCGCATGATC
GTGGATGTCACCCCATACATGAACCCTTCGCCTTTCACCGTCTCGCCCAACACCCACGTCTCCCAAGTCTTCAACCTGTT
CAGAACGATGGGCCTGCGCCACCTGCCCGTGGTGAACGCTGTGGGAGAGATCGTGGGGATCATCACACGGCACAACCTCA
CCTATGAATTTCTGCAGGCCCGGCTGAGGCAGCACTACCAGACCATCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAG
CCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_CLCN6_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACCCGCACCCCCGA
GGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAAGACTATGAGAGTTTGGATTATG
ATCGCTGTATCAATGACCCTTACCTGGAAGTTTTGGAGACCATGGATAATAAGAAAGGTCGAAGATATGAGGCGGTGAAG
TGGATGGTGGTGTTTGCCATTGGAGTCTGCACTGGCCTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCA
ACTCAAGTTCGGAGTGGTACAGACATCGGTGGAGGAGTGCAGCCAGAAAGGCTGCCTCGCTCTGTCTCTCCTTGAACTCC
TGGGTTTTAACCTCACCTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGGATACCC
GAGGTCAAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAGGTCCTTGGAGT
GCTGTTCAGTGTGGCTGGAGGGCTCTTCGTGGGGAAGGAAGGCCCCATGATCCACAGTGGTTCGGTGGTGGGAGCTGGCC
TCCCTCAGTTTCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTCCCCTATTTCCGAAGCGACAGAGACAAGAGAGAC
TTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCAGCTTTCGGGGCGCCAATCGGGGGTACCTTGTTCAGTCTAGAGGA
GGGTTCGTCCTTCTGGAACCAAGGGCTCACGTGGAAAGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCAACTTCT
TCCATTCTGGGATTCAGTTTGGAAGCTGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGTGCTCT
GACTCTGATAAAAAATGTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGGGGCCTCCT
GGGAGCCACATTCAACTGTCTGAACAAGAGGCTTGCAAAGTACCGTATGCGAAACGTGCACCCGAAACCTAAGCTCGTCA
GAGTCTTAGAGAGCCTCCTTGTGTCTCTGGTAACCACCGTGGTGGTGTTTGTGGCCTCGATGGTGTTAGGAGAATGCCGA
CAGATGTCCTCTTCGAGTCAAATCGGTAATGACTCATTCCAGCTCCAGGTCACAGAAGATGTGAATTCAAGTATCAAGAC
ATTTTTTTGTCCCAATGATACCTACAATGACATGGCCACACTCTTCTTCAACCCGCAGGAGTCTGCCATCCTCCAGCTCT
TCCACCAGGATGGTACTTTCAGCCCCGTCACTCTGGCCTTGTTCTTCGTTCTCTATTTCTTGCTTGCATGTTGGACTTAC
GGCATTTCTGTTCCAAGTGGCCTTTTTGTGCCTTCTCTGCTGTGTGGAGCTGCTTTTGGACGTTTAGTTGCCAATGTCCT
AAAAAGCTACATTGGATTGGGCCACATCTATTCGGGGACCTTTGCCCTGATTGGTGCAGCGGCTTTCTTGGGCGGGGTGG
TCCGCATGACCATCAGCCTCACGGTCATCCTGATCGAGTCCACCAATGAGATCACCTACGGGCTCCCCATCATGGTCACA
CTGATGGTGGCCAAATGGACAGGGGACTTTTTCAATAAGGGCATTTATGATATCCACGTGGGCCTGCGAGGCGTGCCGCT
TCTGGAATGGGAGACAGAGGTGGAAATGGACAAGCTGAGAGCCAGCGACATCATGGAGCCCAACCTGACCTACGTCTACC
CGCACACCCGCATCCAGTCTCTGGTGAGCATCCTGCGCACCACGGTCCACCATGCCTTCCCGGTGGTCACAGAGAACCGC
GGTAACGAGAAGGAGTTCATGAAGGGCAACCAGCTCATCAGCAACAACATCAAGTTCAAGAAATCCAGCATCCTCACCCG
GGCTGGCGAGCAGCGCAAACGGAGCCAGTCCATGAAGTCCTACCCATCCAGCGAGCTACGGAACATGTGTGATGAGCACA
TCGCCTCTGAGGAGCCAGCCGAGAAGGAGGACCTCCTGCAGCAGATGCTGGAAAGGAGATACACTCCCTACCCCAACCTA
TACCCTGACCAGTCCCCAAGTGAAGACTGGACCATGGAGGAGCGGTTCCGCCCTCTGACCTTCCACGGCCTGATCCTTCG
GTCGCAGCTTGTCACCCTGCTTGTCCGAGGAGTTTGTTACTCTGAAAGCCAGTCGAGCGCCAGCCAGCCGCGCCTCTCCT
ATGCCGAGATGGCCGAGGACTACCCGCGGTACCCCGACATCCACGACCTGGACCTGACGCTGCTCAACCCGCGCATGATC
GTGGATGTCACCCCATACATGAACCCTTCGCCTTTCACCGTCTCGCCCAACACCCACGTCTCCCAAGTCTTCAACCTGTT
CAGAACGATGGGCCTGCGCCACCTGCCCGTGGTGAACGCTGTGGGAGAGATCGTGGGGATCATCACACGGCACAACCTCA
CCTATGAATTTCTGCAGGCCCGGCTGAGGCAGCACTACCAGACCATCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAG
CCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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