Over Expression Cells for CLIC2

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

CLIC2

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-CLIC2-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Single cell clones: F8, G10

Subcellular localization: Cytoplasmic

Sequence

ORF Sequence:

ATGTCAGGCCTGCGGCCCGGCACTCAAGTGGACCCTGAGATTGAGCTTTTTGTAAAGGCTGGAAGTGATGGAGAGAGTAT TGGAAACTGTCCCTTTTGCCAACGCCTTTTCATGATCCTCTGGCTTAAAGGAGTTAAATTTAATGTGACAACTGTTGACA TGACCAGAAAGCCTGAAGAACTAAAGGACTTAGCCCCAGGTACCAATCCTCCGTTCCTGGTGTATAACAAGGAGTTGAAA ACAGACTTCATTAAAATTGAGGAGTTTTTAGAACAAACCCTGGCTCCTCCAAGGTACCCTCACCTGAGTCCCAAGTACAA GGAGTCTTTTGATGTGGGCTGTAACCTCTTTGCCAAGTTTTCTGCATACATTAAGAATACACAAAAGGAGGCAAATAAGA ATTTTGAAAAATCTCTGCTCAAAGAATTCAAGCGTCTGGATGACTACTTAAACACCCCACTTCTGGATGAAATTGATCCA GACAGTGCTGAGGAACCCCCAGTTTCCAGAAGACTATTCTTGGATGGGGACCAGCTAACACTGGCTGATTGTAGCTTGTT ACCCAAGCTGAACATTATTAAAGTTGCTGCCAAGAAATATCGTGACTTTGACATTCCAGCAGAATTCTCAGGAGTCTGGC GTTATCTCCACAATGCCTATGCCCGTGAAGAATTTACCCACACGTGTCCTGAAGACAAAGAAATTGAAAATACTTACGCA AATGTGGCTAAACAGAAGAGTTACCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCT CGATTCTACGTAG

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