10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for CLIC2 Gene
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CLIC2-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single cell clones: F8, G10
Subcellular localization: Cytoplasmic
Sequence
ORF Sequence:
ATGTCAGGCCTGCGGCCCGGCACTCAAGTGGACCCTGAGATTGAGCTTTTTGTAAAGGCTGGAAGTGATGGAGAGAGTAT TGGAAACTGTCCCTTTTGCCAACGCCTTTTCATGATCCTCTGGCTTAAAGGAGTTAAATTTAATGTGACAACTGTTGACA TGACCAGAAAGCCTGAAGAACTAAAGGACTTAGCCCCAGGTACCAATCCTCCGTTCCTGGTGTATAACAAGGAGTTGAAA ACAGACTTCATTAAAATTGAGGAGTTTTTAGAACAAACCCTGGCTCCTCCAAGGTACCCTCACCTGAGTCCCAAGTACAA GGAGTCTTTTGATGTGGGCTGTAACCTCTTTGCCAAGTTTTCTGCATACATTAAGAATACACAAAAGGAGGCAAATAAGA ATTTTGAAAAATCTCTGCTCAAAGAATTCAAGCGTCTGGATGACTACTTAAACACCCCACTTCTGGATGAAATTGATCCA GACAGTGCTGAGGAACCCCCAGTTTCCAGAAGACTATTCTTGGATGGGGACCAGCTAACACTGGCTGATTGTAGCTTGTT ACCCAAGCTGAACATTATTAAAGTTGCTGCCAAGAAATATCGTGACTTTGACATTCCAGCAGAATTCTCAGGAGTCTGGC GTTATCTCCACAATGCCTATGCCCGTGAAGAATTTACCCACACGTGTCCTGAAGACAAAGAAATTGAAAATACTTACGCA AATGTGGCTAAACAGAAGAGTTACCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCT CGATTCTACGTAG
Media and Files
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_CLIC2_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGTCAGGCCTGCGGCCCGGCACTCAAGTGGACCCTGAGATTGAGCTTTTTGTAAAGGCTGGAAGTGATGGAGAGAGTAT
TGGAAACTGTCCCTTTTGCCAACGCCTTTTCATGATCCTCTGGCTTAAAGGAGTTAAATTTAATGTGACAACTGTTGACA
TGACCAGAAAGCCTGAAGAACTAAAGGACTTAGCCCCAGGTACCAATCCTCCGTTCCTGGTGTATAACAAGGAGTTGAAA
ACAGACTTCATTAAAATTGAGGAGTTTTTAGAACAAACCCTGGCTCCTCCAAGGTACCCTCACCTGAGTCCCAAGTACAA
GGAGTCTTTTGATGTGGGCTGTAACCTCTTTGCCAAGTTTTCTGCATACATTAAGAATACACAAAAGGAGGCAAATAAGA
ATTTTGAAAAATCTCTGCTCAAAGAATTCAAGCGTCTGGATGACTACTTAAACACCCCACTTCTGGATGAAATTGATCCA
GACAGTGCTGAGGAACCCCCAGTTTCCAGAAGACTATTCTTGGATGGGGACCAGCTAACACTGGCTGATTGTAGCTTGTT
ACCCAAGCTGAACATTATTAAAGTTGCTGCCAAGAAATATCGTGACTTTGACATTCCAGCAGAATTCTCAGGAGTCTGGC
GTTATCTCCACAATGCCTATGCCCGTGAAGAATTTACCCACACGTGTCCTGAAGACAAAGAAATTGAAAATACTTACGCA
AATGTGGCTAAACAGAAGAGTTACCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCT
CGATTCTACGTAG
TGGAAACTGTCCCTTTTGCCAACGCCTTTTCATGATCCTCTGGCTTAAAGGAGTTAAATTTAATGTGACAACTGTTGACA
TGACCAGAAAGCCTGAAGAACTAAAGGACTTAGCCCCAGGTACCAATCCTCCGTTCCTGGTGTATAACAAGGAGTTGAAA
ACAGACTTCATTAAAATTGAGGAGTTTTTAGAACAAACCCTGGCTCCTCCAAGGTACCCTCACCTGAGTCCCAAGTACAA
GGAGTCTTTTGATGTGGGCTGTAACCTCTTTGCCAAGTTTTCTGCATACATTAAGAATACACAAAAGGAGGCAAATAAGA
ATTTTGAAAAATCTCTGCTCAAAGAATTCAAGCGTCTGGATGACTACTTAAACACCCCACTTCTGGATGAAATTGATCCA
GACAGTGCTGAGGAACCCCCAGTTTCCAGAAGACTATTCTTGGATGGGGACCAGCTAACACTGGCTGATTGTAGCTTGTT
ACCCAAGCTGAACATTATTAAAGTTGCTGCCAAGAAATATCGTGACTTTGACATTCCAGCAGAATTCTCAGGAGTCTGGC
GTTATCTCCACAATGCCTATGCCCGTGAAGAATTTACCCACACGTGTCCTGAAGACAAAGAAATTGAAAATACTTACGCA
AATGTGGCTAAACAGAAGAGTTACCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCT
CGATTCTACGTAG
Media and Files
Snapgene File: