Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGGCCCACGTGAGACACTTTCGGACATTAGTTTCGGGATTTTACTTCTGGGAAGCAGCACTGTTACTCAGTTTGGTTGC
CACAAAGGAAACAGACAGTGCAAGATCTCGAAGTGCTCCAATGTCACCTTCTGATTTTCTGGATAAATTAATGGGCAGGA
CATCAGGATATGATGCAAGAATCAGACCCAATTTTAAAGGCCCTCCAGTTAATGTCACATGCAACATATTCATCAACAGC
TTTGGCTCTATCGCAGAGACGACCATGGATTACAGAGTGAATATCTTTCTTCGTCAGAAATGGAATGATCCCCGCCTCGC
GTACAGTGAATATCCTGACGACTCTTTAGACCTCGACCCCTCCATGTTGGACTCCATTTGGAAACCTGATTTGTTCTTTG
CCAATGAAAAGGGTGCCAACTTTCATGAAGTCACTACAGACAACAAATTGCTAAGAATTTTCAAAAATGGAAATGTTCTT
TATTCAATAAGATTAACATTAACACTTTCCTGTCCAATGGATCTCAAGAATTTTCCCATGGATGTACAAACATGTATAAT
GCAACTGGAAAGCTTTGGGTACACAATGAATGATCTCATTTTTGAATGGCAAGATGAGGCACCCGTACAAGTGGCAGAAG
GACTCACTTTGCCCCAGTTTCTGTTGAAAGAAGAAAAAGATTTACGATACTGCACTAAACATTACAATACAGGAAAGTTT
ACGTGTATAGAAGTGCGATTCCATCTGGAGCGACAAATGGGATACTATCTGATCCAGATGTACATTCCCAGTCTCCTGAT
TGTTATTCTATCCTGGGTTTCGTTCTGGATCAACATGGATGCAGCACCGGCCAGGGTAGCTCTGGGGATAACCACCGTGC
TAACGATGACTACACAGAGTTCAGGATCACGAGCTTCCTTGCCAAAAGTTTCATATGTCAAAGCTATTGATATTTGGATG
GCAGTATGCCTCCTTTTTGTGTTTTCAGCACTTCTGGAGTATGCAGCTGTAAATTTTGTATCAAGACAACACAAAGAACT
TCTGAGATTTCGACGAAAGAGAAAGAATAAGACAGAAGCCTTTGCACTGGAGAAGTTTTACCGTTTCTCAGATATGGATG
ATGAGGTAAGGGAAAGCCGATTCAGCTTCACAGCCTATGGAATGGGACCATGTCTACAAGCAAAGGATGGCATGACTCCA
AAGGGCCCCAACCACCCTGTCCAGGTAATGCCAAAAAGTCCTGATGAAATGAGGAAGGTCTTTATCGACCGGGCCAAGAA
GATTGATACCATCTCCCGAGCCTGCTTCCCATTAGCTTTTTTGATTTTTAATATTTTCTACTGGGTTATCTATAAAATTC
TTAGGCATGAGGATATTCATCAGCAGCAAGATACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTC
CTCGGTCTCGATTCTACGTAG
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