Over Expression Cells for KCNA6

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

KCNA6

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-KCNA6-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Single Cell Clones:

Possibly C6

V5 Staining appears to be intracellular. Cell population is heterogenous

Sequence

ORF Sequence:

ATGAGATCGGAGAAATCCCTTACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGAGAGCAACAGGATGCGGGAGA CTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATATCTCCGGGCTGCGCTTTGAGA CACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCTGGCCGGCGAGTCCGCTTCTTCGACCCCCTG AGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCG GAGGCCGGTCAACGTGCCCCTGGACATTTTCCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCT TCCGGGAGGACGAGGGCTGCCTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTG TGGCTGCTCTTTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGTGTGAGTCGAG TCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACATTTCATCATGGCATCACCCCT GGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTCCTTCTTTACAGACCCCTTCTTTCTGGTGGA GACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTGCGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGA ACATCATGAACATCATTGACTTGGTGGCTATCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAG CAGCAACCAGCCAGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGT CCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGAC GATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATGACCACGGTAGGTTACGGGGA CATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCATCGCTGGGGTCCTCACCATTGCCCTGCCTG TGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTC ACTTGTGGGCAGCCTGCGCCGGACCTGAGGGCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGA ACGGAGACCCAGCTACCTTCCTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTCTGCCCAACTTTCT TGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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