Skip to main content
Loading...

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNA6-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

 

Single Cell Clones: 

Possibly C6

V5 Staining appears to be intracellular. Cell population is heterogenous

Sequence

ORF Sequence:

ATGAGATCGGAGAAATCCCTTACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGAGAGCAACAGGATGCGGGAGA CTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATATCTCCGGGCTGCGCTTTGAGA CACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCTGGCCGGCGAGTCCGCTTCTTCGACCCCCTG AGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCG GAGGCCGGTCAACGTGCCCCTGGACATTTTCCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCT TCCGGGAGGACGAGGGCTGCCTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTG TGGCTGCTCTTTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGTGTGAGTCGAG TCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACATTTCATCATGGCATCACCCCT GGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTCCTTCTTTACAGACCCCTTCTTTCTGGTGGA GACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTGCGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGA ACATCATGAACATCATTGACTTGGTGGCTATCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAG CAGCAACCAGCCAGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGT CCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGAC GATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATGACCACGGTAGGTTACGGGGA CATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCATCGCTGGGGTCCTCACCATTGCCCTGCCTG TGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTC ACTTGTGGGCAGCCTGCGCCGGACCTGAGGGCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGA ACGGAGACCCAGCTACCTTCCTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTCTGCCCAACTTTCT TGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNA6_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGAGATCGGAGAAATCCCTTACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGAGAGCAACAGGATGCGGGAGA
CTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATATCTCCGGGCTGCGCTTTGAGA
CACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCTGGCCGGCGAGTCCGCTTCTTCGACCCCCTG
AGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCG
GAGGCCGGTCAACGTGCCCCTGGACATTTTCCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCT
TCCGGGAGGACGAGGGCTGCCTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTG
TGGCTGCTCTTTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC
CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGTGTGAGTCGAG
TCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACATTTCATCATGGCATCACCCCT
GGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTCCTTCTTTACAGACCCCTTCTTTCTGGTGGA
GACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTGCGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGA
ACATCATGAACATCATTGACTTGGTGGCTATCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAG
CAGCAACCAGCCAGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGT
CCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA
GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGAC
GATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATGACCACGGTAGGTTACGGGGA
CATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCATCGCTGGGGTCCTCACCATTGCCCTGCCTG
TGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTC
ACTTGTGGGCAGCCTGCGCCGGACCTGAGGGCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGA
ACGGAGACCCAGCTACCTTCCTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTCTGCCCAACTTTCT
TGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files