10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for KCNA6 Gene
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNA6-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single Cell Clones:
Possibly C6
V5 Staining appears to be intracellular. Cell population is heterogenous
Sequence
ORF Sequence:
ATGAGATCGGAGAAATCCCTTACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGAGAGCAACAGGATGCGGGAGA CTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATATCTCCGGGCTGCGCTTTGAGA CACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCTGGCCGGCGAGTCCGCTTCTTCGACCCCCTG AGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCG GAGGCCGGTCAACGTGCCCCTGGACATTTTCCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCT TCCGGGAGGACGAGGGCTGCCTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTG TGGCTGCTCTTTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGTGTGAGTCGAG TCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACATTTCATCATGGCATCACCCCT GGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTCCTTCTTTACAGACCCCTTCTTTCTGGTGGA GACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTGCGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGA ACATCATGAACATCATTGACTTGGTGGCTATCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAG CAGCAACCAGCCAGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGT CCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGAC GATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATGACCACGGTAGGTTACGGGGA CATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCATCGCTGGGGTCCTCACCATTGCCCTGCCTG TGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTC ACTTGTGGGCAGCCTGCGCCGGACCTGAGGGCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGA ACGGAGACCCAGCTACCTTCCTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTCTGCCCAACTTTCT TGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Basic Information
Gene Taxonomy:
Injury Type:
Neuron Type:
Tissue:
DRG
Conditions:
naive vs injury (SNI 7days)
Probe:
Probe Mm-Kcna6 #462821 At Advanced Cell Diagnostics
Species:
Mouse
Media and Files
Normal Tissue Image:
Expression level comparison:
Injured Tissue Image:
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNA6_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGAGATCGGAGAAATCCCTTACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGAGAGCAACAGGATGCGGGAGA
CTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATATCTCCGGGCTGCGCTTTGAGA
CACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCTGGCCGGCGAGTCCGCTTCTTCGACCCCCTG
AGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCG
GAGGCCGGTCAACGTGCCCCTGGACATTTTCCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCT
TCCGGGAGGACGAGGGCTGCCTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTG
TGGCTGCTCTTTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC
CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGTGTGAGTCGAG
TCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACATTTCATCATGGCATCACCCCT
GGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTCCTTCTTTACAGACCCCTTCTTTCTGGTGGA
GACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTGCGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGA
ACATCATGAACATCATTGACTTGGTGGCTATCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAG
CAGCAACCAGCCAGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGT
CCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA
GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGAC
GATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATGACCACGGTAGGTTACGGGGA
CATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCATCGCTGGGGTCCTCACCATTGCCCTGCCTG
TGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTC
ACTTGTGGGCAGCCTGCGCCGGACCTGAGGGCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGA
ACGGAGACCCAGCTACCTTCCTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTCTGCCCAACTTTCT
TGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
CTTCCCGGAGGCCGGCGGGGGCGGGGGCTGCTGTAGTAGCGAGCGGCTGGTGATCAATATCTCCGGGCTGCGCTTTGAGA
CACAATTGCGCACCCTGTCGCTGTTTCCGGACACGCTGCTCGGAGACCCTGGCCGGCGAGTCCGCTTCTTCGACCCCCTG
AGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCTGGGGGCCGCCTGCG
GAGGCCGGTCAACGTGCCCCTGGACATTTTCCTGGAGGAGATCCGCTTCTACCAGCTGGGGGACGAGGCCCTGGCGGCCT
TCCGGGAGGACGAGGGCTGCCTGCCCGAAGGTGGCGAGGACGAGAAGCCGCTGCCCTCCCAGCCCTTCCAGCGCCAGGTG
TGGCTGCTCTTTGAGTACCCAGAGAGCTCTGGGCCGGCCAGGGGCATCGCCATCGTCTCCGTGTTGGTCATTCTCATCTC
CATAGTCATCTTTTGCCTGGAGACCTTACCCCAGTTCCGTGTAGATGGTCGAGGTGGAAACAATGGTGGTGTGAGTCGAG
TCTCCCCAGTTTCCAGGGGGAGTCAGGAGGAAGAGGAGGATGAAGACGATTCCTACACATTTCATCATGGCATCACCCCT
GGGGAAATGGGGACCGGGGGCTCCTCCTCACTCAGTACTCTTGGGGGCTCCTTCTTTACAGACCCCTTCTTTCTGGTGGA
GACGCTGTGCATTGTCTGGTTCACTTTTGAGCTCCTGGTGCGCTTCTCCGCCTGCCCTAGCAAGCCGGCCTTCTTCCGGA
ACATCATGAACATCATTGACTTGGTGGCTATCTTCCCCTACTTCATCACCCTGGGCACTGAGCTGGTGCAGCAGCAGGAG
CAGCAACCAGCCAGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGCCATCCTCCGAGTCATCCGCCTGGT
CCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTGCAGATCCTGGGCAAGACCTTGCAGGCCTCCATGA
GGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCGGGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGAC
GATGACGATTCGCTTTTTCCCAGCATCCCGGATGCCTTCTGGTGGGCAGTGGTTACAATGACCACGGTAGGTTACGGGGA
CATGTACCCCATGACTGTGGGGGGAAAGATCGTGGGCTCGCTGTGTGCCATCGCTGGGGTCCTCACCATTGCCCTGCCTG
TGCCCGTCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAGACGGAGCAGGAGGAGCAAGGCCAGTATACCCACGTC
ACTTGTGGGCAGCCTGCGCCGGACCTGAGGGCAACTGACAACGGACTTGGCAAGCCTGACTTCCCCGAGGCTAACCGGGA
ACGGAGACCCAGCTACCTTCCTACACCACATCGGGCCTATGCAGAGAAAAGAATGCTCACGGAGGTCTGCCCAACTTTCT
TGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Snapgene File:
Basic Information
Gene Taxonomy:
Construct ID:
IDG_KCNA6_UE_1
Virus Type:
Selection:
Puro,Amp
Vector:
783_hU6_EGFP
Passed QC:
On
Vector Type:
Sequence
Inserted_sequence:
GTCTGGCGCTTAGGTACCAG
Media and Files
Vector Map:
Snapgene File: