Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGCAGGTGTCTATCGCGTGTACCGAGCAGAACCTTCGCAGCCGGAGCAGTGAGGACCGTCTGTGTGGACCCCGGCCGGG
CCCCGGGGGCGGTAATGGTGGGCCGGCCGGCGGGGGGCACGGGAATCCTCCGGGGGGTGGAGGGTCTGGCCCCAAGGCCC
GAGCTGCACTGGTTCCCCGACCCCCAGCGCCCGCTGGGGCCCTCCGAGAGAGCACCGGCCGAGGCACTGGCATGAAATAC
AGGAACCTAGGGAAGTCTGGTCTTCGGGTATCCTGTCTTGGCCTAGGTACCTGGGTCACATTTGGTTCTCAGATCTCAGA
TGAGACAGCAGAGGATGTGCTGACTGTAGCCTATGAGCATGGTGTAAACCTGTTTGACACCGCCGAAGTGTACGCAGCAG
GAAAGGCTGAAAGAACCCTAGGGAACATCCTCAAGAGCAAAGGTTGGAGGAGATCAAGCTATGTCATCACTACCAAGATT
TTTTGGGGAGGACAGGCAGAAACCGAGCGAGGTTTAAGCCGAAAGCACATCATTGAGGGCTTGCGAGGATCCCTGGAACG
CCTCCAGCTGGGATACGTGGACATTGTCTTTGCCAATCGCTCAGACCCCAACTGTCCTATGGAGGAGATTGTGCGAGCCA
TGACCTATGTCATCAACCAGGGCCTGGCCCTATACTGGGGGACATCCCGATGGGGGGCTGCAGAAATCATGGAGGCCTAC
TCCATGGCCAGACAGTTCAATCTGATTCCTCCAGTGTGTGAACAAGCGGAGCACCATCTGTTTCAGAGGGAGAAGGTGGA
GATGCAGCTGCCAGAGCTCTACCACAAGATTGGAGTTGGATCAGTCACTTGGTACCCTCTAGCCTGTGGTCTCATTACTA
GCAAGTATGATGGGCGAGTCCCAGATACTTGCAGGGCCTCCATCAAGGGCTACCAGTGGCTCAAGGACAAAGTGCAGAGT
GAAGATGGCAAGAAGCAACAAGCCAAAGTCATGGACCTTCTTCCTGTCGCTCACCAGCTGGGCTGCACCGTGGCCCAGCT
TGCTATTGCGTGGTGTCTCCGCAGTGAGGGTGTCAGCTCTGTCTTGCTGGGGGTGTCGAGTGCGGAGCAGTTGATAGAAC
ACCTGGGCGCGCTACAGGTGCTGAGCCAGCTGACCCCGCAGACAGTGATGGAAATAGACGGGCTCCTGGGAAACAAGCCG
CATTCCAAGAAGTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTAC
GTAG
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