10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for KCNAB3 Gene
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNAB3-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ORF Sequence:
ATGCAGGTGTCTATCGCGTGTACCGAGCAGAACCTTCGCAGCCGGAGCAGTGAGGACCGTCTGTGTGGACCCCGGCCGGG
CCCCGGGGGCGGTAATGGTGGGCCGGCCGGCGGGGGGCACGGGAATCCTCCGGGGGGTGGAGGGTCTGGCCCCAAGGCCC
GAGCTGCACTGGTTCCCCGACCCCCAGCGCCCGCTGGGGCCCTCCGAGAGAGCACCGGCCGAGGCACTGGCATGAAATAC
AGGAACCTAGGGAAGTCTGGTCTTCGGGTATCCTGTCTTGGCCTAGGTACCTGGGTCACATTTGGTTCTCAGATCTCAGA
TGAGACAGCAGAGGATGTGCTGACTGTAGCCTATGAGCATGGTGTAAACCTGTTTGACACCGCCGAAGTGTACGCAGCAG
GAAAGGCTGAAAGAACCCTAGGGAACATCCTCAAGAGCAAAGGTTGGAGGAGATCAAGCTATGTCATCACTACCAAGATT
TTTTGGGGAGGACAGGCAGAAACCGAGCGAGGTTTAAGCCGAAAGCACATCATTGAGGGCTTGCGAGGATCCCTGGAACG
CCTCCAGCTGGGATACGTGGACATTGTCTTTGCCAATCGCTCAGACCCCAACTGTCCTATGGAGGAGATTGTGCGAGCCA
TGACCTATGTCATCAACCAGGGCCTGGCCCTATACTGGGGGACATCCCGATGGGGGGCTGCAGAAATCATGGAGGCCTAC
TCCATGGCCAGACAGTTCAATCTGATTCCTCCAGTGTGTGAACAAGCGGAGCACCATCTGTTTCAGAGGGAGAAGGTGGA
GATGCAGCTGCCAGAGCTCTACCACAAGATTGGAGTTGGATCAGTCACTTGGTACCCTCTAGCCTGTGGTCTCATTACTA
GCAAGTATGATGGGCGAGTCCCAGATACTTGCAGGGCCTCCATCAAGGGCTACCAGTGGCTCAAGGACAAAGTGCAGAGT
GAAGATGGCAAGAAGCAACAAGCCAAAGTCATGGACCTTCTTCCTGTCGCTCACCAGCTGGGCTGCACCGTGGCCCAGCT
TGCTATTGCGTGGTGTCTCCGCAGTGAGGGTGTCAGCTCTGTCTTGCTGGGGGTGTCGAGTGCGGAGCAGTTGATAGAAC
ACCTGGGCGCGCTACAGGTGCTGAGCCAGCTGACCCCGCAGACAGTGATGGAAATAGACGGGCTCCTGGGAAACAAGCCG
CATTCCAAGAAGTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTAC
GTAG
Media and Files
Basic Information
Gene Taxonomy:
Injury Type:
Neuron Type:
Tissue:
DRG
Conditions:
naive vs injury (SNI 7days)
Probe:
Probe Mm-Kcnab3 #479081 At Advanced Cell Diagnostics
Species:
Mouse
Media and Files
Normal Tissue Image:
Expression level comparison:
Injured Tissue Image:
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNAB3_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGCAGGTGTCTATCGCGTGTACCGAGCAGAACCTTCGCAGCCGGAGCAGTGAGGACCGTCTGTGTGGACCCCGGCCGGG
CCCCGGGGGCGGTAATGGTGGGCCGGCCGGCGGGGGGCACGGGAATCCTCCGGGGGGTGGAGGGTCTGGCCCCAAGGCCC
GAGCTGCACTGGTTCCCCGACCCCCAGCGCCCGCTGGGGCCCTCCGAGAGAGCACCGGCCGAGGCACTGGCATGAAATAC
AGGAACCTAGGGAAGTCTGGTCTTCGGGTATCCTGTCTTGGCCTAGGTACCTGGGTCACATTTGGTTCTCAGATCTCAGA
TGAGACAGCAGAGGATGTGCTGACTGTAGCCTATGAGCATGGTGTAAACCTGTTTGACACCGCCGAAGTGTACGCAGCAG
GAAAGGCTGAAAGAACCCTAGGGAACATCCTCAAGAGCAAAGGTTGGAGGAGATCAAGCTATGTCATCACTACCAAGATT
TTTTGGGGAGGACAGGCAGAAACCGAGCGAGGTTTAAGCCGAAAGCACATCATTGAGGGCTTGCGAGGATCCCTGGAACG
CCTCCAGCTGGGATACGTGGACATTGTCTTTGCCAATCGCTCAGACCCCAACTGTCCTATGGAGGAGATTGTGCGAGCCA
TGACCTATGTCATCAACCAGGGCCTGGCCCTATACTGGGGGACATCCCGATGGGGGGCTGCAGAAATCATGGAGGCCTAC
TCCATGGCCAGACAGTTCAATCTGATTCCTCCAGTGTGTGAACAAGCGGAGCACCATCTGTTTCAGAGGGAGAAGGTGGA
GATGCAGCTGCCAGAGCTCTACCACAAGATTGGAGTTGGATCAGTCACTTGGTACCCTCTAGCCTGTGGTCTCATTACTA
GCAAGTATGATGGGCGAGTCCCAGATACTTGCAGGGCCTCCATCAAGGGCTACCAGTGGCTCAAGGACAAAGTGCAGAGT
GAAGATGGCAAGAAGCAACAAGCCAAAGTCATGGACCTTCTTCCTGTCGCTCACCAGCTGGGCTGCACCGTGGCCCAGCT
TGCTATTGCGTGGTGTCTCCGCAGTGAGGGTGTCAGCTCTGTCTTGCTGGGGGTGTCGAGTGCGGAGCAGTTGATAGAAC
ACCTGGGCGCGCTACAGGTGCTGAGCCAGCTGACCCCGCAGACAGTGATGGAAATAGACGGGCTCCTGGGAAACAAGCCG
CATTCCAAGAAGTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTAC
GTAG
CCCCGGGGGCGGTAATGGTGGGCCGGCCGGCGGGGGGCACGGGAATCCTCCGGGGGGTGGAGGGTCTGGCCCCAAGGCCC
GAGCTGCACTGGTTCCCCGACCCCCAGCGCCCGCTGGGGCCCTCCGAGAGAGCACCGGCCGAGGCACTGGCATGAAATAC
AGGAACCTAGGGAAGTCTGGTCTTCGGGTATCCTGTCTTGGCCTAGGTACCTGGGTCACATTTGGTTCTCAGATCTCAGA
TGAGACAGCAGAGGATGTGCTGACTGTAGCCTATGAGCATGGTGTAAACCTGTTTGACACCGCCGAAGTGTACGCAGCAG
GAAAGGCTGAAAGAACCCTAGGGAACATCCTCAAGAGCAAAGGTTGGAGGAGATCAAGCTATGTCATCACTACCAAGATT
TTTTGGGGAGGACAGGCAGAAACCGAGCGAGGTTTAAGCCGAAAGCACATCATTGAGGGCTTGCGAGGATCCCTGGAACG
CCTCCAGCTGGGATACGTGGACATTGTCTTTGCCAATCGCTCAGACCCCAACTGTCCTATGGAGGAGATTGTGCGAGCCA
TGACCTATGTCATCAACCAGGGCCTGGCCCTATACTGGGGGACATCCCGATGGGGGGCTGCAGAAATCATGGAGGCCTAC
TCCATGGCCAGACAGTTCAATCTGATTCCTCCAGTGTGTGAACAAGCGGAGCACCATCTGTTTCAGAGGGAGAAGGTGGA
GATGCAGCTGCCAGAGCTCTACCACAAGATTGGAGTTGGATCAGTCACTTGGTACCCTCTAGCCTGTGGTCTCATTACTA
GCAAGTATGATGGGCGAGTCCCAGATACTTGCAGGGCCTCCATCAAGGGCTACCAGTGGCTCAAGGACAAAGTGCAGAGT
GAAGATGGCAAGAAGCAACAAGCCAAAGTCATGGACCTTCTTCCTGTCGCTCACCAGCTGGGCTGCACCGTGGCCCAGCT
TGCTATTGCGTGGTGTCTCCGCAGTGAGGGTGTCAGCTCTGTCTTGCTGGGGGTGTCGAGTGCGGAGCAGTTGATAGAAC
ACCTGGGCGCGCTACAGGTGCTGAGCCAGCTGACCCCGCAGACAGTGATGGAAATAGACGGGCTCCTGGGAAACAAGCCG
CATTCCAAGAAGTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTAC
GTAG
Media and Files
Snapgene File:
Basic Information
Gene Taxonomy:
Construct ID:
IDG_KCNAB3_UE_1
Virus Type:
Selection:
Puro,Amp
Vector:
783_hU6_EGFP
Passed QC:
On
Vector Type:
Sequence
Inserted_sequence:
GGATAGCTACCCAGGAACAA
Media and Files
Vector Map:
Snapgene File: