Basic Information
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ATGGGCCTGGCCAGGGCCCTACGCCGCCTCAGCGGCGCCCTGGATTCGGGAGACAGCCGGGCGGGCGATGAAGAGGAGGC
CGGGCCCGGGTTGTGCCGCAACGGGTGGGCGCCGGCACCGGTGCAGTCACCCGTGGGCCGGCGCCGCGGTCGCTTCGTCA
AGAAAGACGGGCACTGCAACGTGCGTTTCGTAAACCTGGGTGGCCAGGGCGCGCGCTACCTGAGCGACCTGTTCACCACA
TGCGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCTCCTGCTCCTTCCTCGCCTCCTGGCTGCTCTTCGGCCTGGC
CTTCTGGCTCATTGCCTCGCTGCACGGCGACCTGGCCGCCCCGCCACCGCCCGCGCCCTGCTTCTCACACGTGGCCAGCT
TCCTGGCCGCCTTCCTCTTCGCGCTGGAGACGCAGACGTCCATCGGCTACGGCGTGCGCAGCGTCACCGAGGAGTGCCCG
GCCGCTGTGGCCGCCGTGGTGCTGCAGTGCATTGCCGGCTGCGTGCTCGACGCCTTCGTCGTGGGTGCTGTCATGGCCAA
GATGGCCAAACCCAAGAAGCGCAACGAGACGCTGGTCTTCAGCGAGAACGCCGTCGTGGCGCTGCGCGACCACCGCCTCT
GCCTCATGTGGCGCGTCGGCAACCTGCGCCGCAGCCACCTGGTCGAGGCCCACGTGCGTGCCCAGCTGCTGCAGCCCCGT
GTGACCCCAGAGGGTGAGTACATCCCGCTGGACCACCAGGATGTGGATGTGGGCTTTGATGGAGGCACCGATCGTATCTT
CCTCGTGTCCCCCATCACCATCGTCCATGAGATCGACTCTGCCAGTCCTCTGTATGAGCTAGGACGTGCCGAGCTGGCCA
GGGCTGACTTTGAGCTGGTGGTCATTCTCGAGGGGATGGTTGAGGCCACAGCCATGACCACACAGTGTCGCTCGTCCTAC
CTCCCTGGTGAACTGCTCTGGGGCCATCGTTTTGAGCCAGTTCTCTTCCAGCGTGGCTCCCAGTATGAGGTCGACTATCG
CCACTTCCATCGCACTTATGAGGTCCCAGGGACACCGGTCTGCAGTGCTAAGGAGCTGGATGAACGGGCAGAGCAGGCTT
CCCACAGCCTCAAGTCTAGTTTCCCCGGCTCTCTGACTGCATTTTGTTATGAGAATGAACTTGCTCTGAGCTGCTGCCAG
GAGGAAGATGAGGACGATGAGACTGAGGAAGGGAATGGGGTGGAAACAGAAGATGGGGCTGCTAGCCCCCGAGTTCTCAC
ACCAACCCTGGCGCTGACCCTGCCTCCATGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCC
TCGGTCTCGATTCTACGTAG
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