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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNJ14-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGGCCTGGCCAGGGCCCTACGCCGCCTCAGCGGCGCCCTGGATTCGGGAGACAGCCGGGCGGGCGATGAAGAGGAGGC
CGGGCCCGGGTTGTGCCGCAACGGGTGGGCGCCGGCACCGGTGCAGTCACCCGTGGGCCGGCGCCGCGGTCGCTTCGTCA
AGAAAGACGGGCACTGCAACGTGCGTTTCGTAAACCTGGGTGGCCAGGGCGCGCGCTACCTGAGCGACCTGTTCACCACA
TGCGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCTCCTGCTCCTTCCTCGCCTCCTGGCTGCTCTTCGGCCTGGC
CTTCTGGCTCATTGCCTCGCTGCACGGCGACCTGGCCGCCCCGCCACCGCCCGCGCCCTGCTTCTCACACGTGGCCAGCT
TCCTGGCCGCCTTCCTCTTCGCGCTGGAGACGCAGACGTCCATCGGCTACGGCGTGCGCAGCGTCACCGAGGAGTGCCCG
GCCGCTGTGGCCGCCGTGGTGCTGCAGTGCATTGCCGGCTGCGTGCTCGACGCCTTCGTCGTGGGTGCTGTCATGGCCAA
GATGGCCAAACCCAAGAAGCGCAACGAGACGCTGGTCTTCAGCGAGAACGCCGTCGTGGCGCTGCGCGACCACCGCCTCT
GCCTCATGTGGCGCGTCGGCAACCTGCGCCGCAGCCACCTGGTCGAGGCCCACGTGCGTGCCCAGCTGCTGCAGCCCCGT
GTGACCCCAGAGGGTGAGTACATCCCGCTGGACCACCAGGATGTGGATGTGGGCTTTGATGGAGGCACCGATCGTATCTT
CCTCGTGTCCCCCATCACCATCGTCCATGAGATCGACTCTGCCAGTCCTCTGTATGAGCTAGGACGTGCCGAGCTGGCCA
GGGCTGACTTTGAGCTGGTGGTCATTCTCGAGGGGATGGTTGAGGCCACAGCCATGACCACACAGTGTCGCTCGTCCTAC
CTCCCTGGTGAACTGCTCTGGGGCCATCGTTTTGAGCCAGTTCTCTTCCAGCGTGGCTCCCAGTATGAGGTCGACTATCG
CCACTTCCATCGCACTTATGAGGTCCCAGGGACACCGGTCTGCAGTGCTAAGGAGCTGGATGAACGGGCAGAGCAGGCTT
CCCACAGCCTCAAGTCTAGTTTCCCCGGCTCTCTGACTGCATTTTGTTATGAGAATGAACTTGCTCTGAGCTGCTGCCAG
GAGGAAGATGAGGACGATGAGACTGAGGAAGGGAATGGGGTGGAAACAGAAGATGGGGCTGCTAGCCCCCGAGTTCTCAC
ACCAACCCTGGCGCTGACCCTGCCTCCATGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCC
TCGGTCTCGATTCTACGTAG

Media and Files

Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNJ14_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGGGCCTGGCCAGGGCCCTACGCCGCCTCAGCGGCGCCCTGGATTCGGGAGACAGCCGGGCGGGCGATGAAGAGGAGGC
CGGGCCCGGGTTGTGCCGCAACGGGTGGGCGCCGGCACCGGTGCAGTCACCCGTGGGCCGGCGCCGCGGTCGCTTCGTCA
AGAAAGACGGGCACTGCAACGTGCGTTTCGTAAACCTGGGTGGCCAGGGCGCGCGCTACCTGAGCGACCTGTTCACCACA
TGCGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCTCCTGCTCCTTCCTCGCCTCCTGGCTGCTCTTCGGCCTGGC
CTTCTGGCTCATTGCCTCGCTGCACGGCGACCTGGCCGCCCCGCCACCGCCCGCGCCCTGCTTCTCACACGTGGCCAGCT
TCCTGGCCGCCTTCCTCTTCGCGCTGGAGACGCAGACGTCCATCGGCTACGGCGTGCGCAGCGTCACCGAGGAGTGCCCG
GCCGCTGTGGCCGCCGTGGTGCTGCAGTGCATTGCCGGCTGCGTGCTCGACGCCTTCGTCGTGGGTGCTGTCATGGCCAA
GATGGCCAAACCCAAGAAGCGCAACGAGACGCTGGTCTTCAGCGAGAACGCCGTCGTGGCGCTGCGCGACCACCGCCTCT
GCCTCATGTGGCGCGTCGGCAACCTGCGCCGCAGCCACCTGGTCGAGGCCCACGTGCGTGCCCAGCTGCTGCAGCCCCGT
GTGACCCCAGAGGGTGAGTACATCCCGCTGGACCACCAGGATGTGGATGTGGGCTTTGATGGAGGCACCGATCGTATCTT
CCTCGTGTCCCCCATCACCATCGTCCATGAGATCGACTCTGCCAGTCCTCTGTATGAGCTAGGACGTGCCGAGCTGGCCA
GGGCTGACTTTGAGCTGGTGGTCATTCTCGAGGGGATGGTTGAGGCCACAGCCATGACCACACAGTGTCGCTCGTCCTAC
CTCCCTGGTGAACTGCTCTGGGGCCATCGTTTTGAGCCAGTTCTCTTCCAGCGTGGCTCCCAGTATGAGGTCGACTATCG
CCACTTCCATCGCACTTATGAGGTCCCAGGGACACCGGTCTGCAGTGCTAAGGAGCTGGATGAACGGGCAGAGCAGGCTT
CCCACAGCCTCAAGTCTAGTTTCCCCGGCTCTCTGACTGCATTTTGTTATGAGAATGAACTTGCTCTGAGCTGCTGCCAG
GAGGAAGATGAGGACGATGAGACTGAGGAAGGGAATGGGGTGGAAACAGAAGATGGGGCTGCTAGCCCCCGAGTTCTCAC
ACCAACCCTGGCGCTGACCCTGCCTCCATGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCC
TCGGTCTCGATTCTACGTAG

Media and Files