Over Expressed Cell Line for KCNS1

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

KCNS1

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-KCNS1-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCTGATGCTGCTGGTCCGGGGAACACACTATGAGAACCTCCGGTCTAAAGTGGTGCTGCCAACACCCCTAGGAGGGAG
GAGCACTGAAACCTTTGTGAGCGAGTTCCCGGGCCCCGACACCGGGATCCGCTGGCGGCGAAGCGACGAGGCGCTGCGCG
TGAACGTGGGTGGCGTGCGGCGGCAGCTGAGCGCGCGCGCCCTGGCGCGCTTCCCGGGCACGCGGCTGGGCCGCCTGCAG
GCCGCGGCGTCGGAGGAGCAGGCGCGGCGCCTGTGCGACGACTACGACGAGGCGGCGCGCGAATTCTACTTCGACCGGCA
CCCGGGCTTCTTCCTGAGCCTGCTGCACTTCTACCGCACTGGCCACCTGCACGTGCTCGACGAGCTGTGCGTCTTCGCCT
TTGGCCAGGAGGCCGACTACTGGGGCCTAGGCGAGAACGCGCTTGCCGCGTGCTGCCGCGCGCGCTACCTGGAGAGGCGG
CTGACCCAGCCGCACGCCTGGGACGAGGACAGCGACACGCCGAGCAGCGTGGACCCGTGCCCCGACGAGATCTCCGACGT
GCAGCGAGAACTGGCGCGCTATGGCGCGGCGCGCTGTGGCCGCCTGCGCCGCCGCCTCTGGCTGACCATGGAGAACCCGG
GCTACTCGCTGCCGAGCAAGCTCTTCAGCTGCGTCTCCATCAGCGTGGTGCTCGCCTCCATCGCCGCCATGTGCATCCAC
AGCCTGCCCGAGTACCAGGCCCGCGAGGCGGCGGCCGCCGTGGCTGCGGTGGCCGCGGGCCGCAGCCCGGAAGGCGTGCG
CGACGACCCGGTGCTGCGACGCCTCGAGTACTTCTGCATCGCCTGGTTCAGCTTCGAGGTGTCGTCGCGCCTCCTGCTGG
CGCCCAGTACGCGCAACTTCTTCTGCCACCCGCTCAACCTCATCGACATTGTGTCTGTGCTGCCCTTCTATCTCACGCTG
CTGGCTGGTGTGGCACTGGGCGACCAGGGCGGCAAGGAGTTCGGCCACCTGGGCAAGGTGGTGCAGGTGTTCCGCCTCAT
GCGCATCTTCCGCGTACTCAAGTTGGCGCGCCATTCCACCGGGCTGCGCTCGCTGGGAGCCACGCTCAAGCACAGCTACC
GTGAGGTGGGCATCTTGCTGCTGTACCTGGCTGTGGGTGTGTCAGTGTTCTCTGGTGTGGCCTACACAGCTGAAAAGGAG
GAGGACGTGGGCTTTAACACCATCCCAGCCTGCTGGTGGTGGGGCACAGTGAGCATGACCACCGTGGGCTATGGGGATGT
GGTGCCAGTGACGGTGGCTGGCAAGCTGGCAGCCTCAGGCTGCATCCTAGGGGGCATCCTGGTGGTAGCACTCCCCATCA
CCATCATCTTCAACAAGTTCTCCCACTTCTACCGGCGCCAGAAGGCTCTGGAGGCAGCCGTGCGCAACAGCAACCACCAA
GAGTTTGAGGACTTGCTGAGCAGCGTTGATGGGGTGTCGGAGGCATCTCTGGAGACATCCCGAGAAACCTCTCAGGAGGG
ACAGTCTGCAGATCTAGAGAGCCAGGCCCCCAGTGAGCCTCCACACCCTCAGATGTATACAGCTTTCTTGTACAAAGTGG
TTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Over Expressed Cell Line for KCNS1

Basic Information

Sequence

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