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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNS1-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCTGATGCTGCTGGTCCGGGGAACACACTATGAGAACCTCCGGTCTAAAGTGGTGCTGCCAACACCCCTAGGAGGGAG
GAGCACTGAAACCTTTGTGAGCGAGTTCCCGGGCCCCGACACCGGGATCCGCTGGCGGCGAAGCGACGAGGCGCTGCGCG
TGAACGTGGGTGGCGTGCGGCGGCAGCTGAGCGCGCGCGCCCTGGCGCGCTTCCCGGGCACGCGGCTGGGCCGCCTGCAG
GCCGCGGCGTCGGAGGAGCAGGCGCGGCGCCTGTGCGACGACTACGACGAGGCGGCGCGCGAATTCTACTTCGACCGGCA
CCCGGGCTTCTTCCTGAGCCTGCTGCACTTCTACCGCACTGGCCACCTGCACGTGCTCGACGAGCTGTGCGTCTTCGCCT
TTGGCCAGGAGGCCGACTACTGGGGCCTAGGCGAGAACGCGCTTGCCGCGTGCTGCCGCGCGCGCTACCTGGAGAGGCGG
CTGACCCAGCCGCACGCCTGGGACGAGGACAGCGACACGCCGAGCAGCGTGGACCCGTGCCCCGACGAGATCTCCGACGT
GCAGCGAGAACTGGCGCGCTATGGCGCGGCGCGCTGTGGCCGCCTGCGCCGCCGCCTCTGGCTGACCATGGAGAACCCGG
GCTACTCGCTGCCGAGCAAGCTCTTCAGCTGCGTCTCCATCAGCGTGGTGCTCGCCTCCATCGCCGCCATGTGCATCCAC
AGCCTGCCCGAGTACCAGGCCCGCGAGGCGGCGGCCGCCGTGGCTGCGGTGGCCGCGGGCCGCAGCCCGGAAGGCGTGCG
CGACGACCCGGTGCTGCGACGCCTCGAGTACTTCTGCATCGCCTGGTTCAGCTTCGAGGTGTCGTCGCGCCTCCTGCTGG
CGCCCAGTACGCGCAACTTCTTCTGCCACCCGCTCAACCTCATCGACATTGTGTCTGTGCTGCCCTTCTATCTCACGCTG
CTGGCTGGTGTGGCACTGGGCGACCAGGGCGGCAAGGAGTTCGGCCACCTGGGCAAGGTGGTGCAGGTGTTCCGCCTCAT
GCGCATCTTCCGCGTACTCAAGTTGGCGCGCCATTCCACCGGGCTGCGCTCGCTGGGAGCCACGCTCAAGCACAGCTACC
GTGAGGTGGGCATCTTGCTGCTGTACCTGGCTGTGGGTGTGTCAGTGTTCTCTGGTGTGGCCTACACAGCTGAAAAGGAG
GAGGACGTGGGCTTTAACACCATCCCAGCCTGCTGGTGGTGGGGCACAGTGAGCATGACCACCGTGGGCTATGGGGATGT
GGTGCCAGTGACGGTGGCTGGCAAGCTGGCAGCCTCAGGCTGCATCCTAGGGGGCATCCTGGTGGTAGCACTCCCCATCA
CCATCATCTTCAACAAGTTCTCCCACTTCTACCGGCGCCAGAAGGCTCTGGAGGCAGCCGTGCGCAACAGCAACCACCAA
GAGTTTGAGGACTTGCTGAGCAGCGTTGATGGGGTGTCGGAGGCATCTCTGGAGACATCCCGAGAAACCTCTCAGGAGGG
ACAGTCTGCAGATCTAGAGAGCCAGGCCCCCAGTGAGCCTCCACACCCTCAGATGTATACAGCTTTCTTGTACAAAGTGG
TTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNS1_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGCTGATGCTGCTGGTCCGGGGAACACACTATGAGAACCTCCGGTCTAAAGTGGTGCTGCCAACACCCCTAGGAGGGAG
GAGCACTGAAACCTTTGTGAGCGAGTTCCCGGGCCCCGACACCGGGATCCGCTGGCGGCGAAGCGACGAGGCGCTGCGCG
TGAACGTGGGTGGCGTGCGGCGGCAGCTGAGCGCGCGCGCCCTGGCGCGCTTCCCGGGCACGCGGCTGGGCCGCCTGCAG
GCCGCGGCGTCGGAGGAGCAGGCGCGGCGCCTGTGCGACGACTACGACGAGGCGGCGCGCGAATTCTACTTCGACCGGCA
CCCGGGCTTCTTCCTGAGCCTGCTGCACTTCTACCGCACTGGCCACCTGCACGTGCTCGACGAGCTGTGCGTCTTCGCCT
TTGGCCAGGAGGCCGACTACTGGGGCCTAGGCGAGAACGCGCTTGCCGCGTGCTGCCGCGCGCGCTACCTGGAGAGGCGG
CTGACCCAGCCGCACGCCTGGGACGAGGACAGCGACACGCCGAGCAGCGTGGACCCGTGCCCCGACGAGATCTCCGACGT
GCAGCGAGAACTGGCGCGCTATGGCGCGGCGCGCTGTGGCCGCCTGCGCCGCCGCCTCTGGCTGACCATGGAGAACCCGG
GCTACTCGCTGCCGAGCAAGCTCTTCAGCTGCGTCTCCATCAGCGTGGTGCTCGCCTCCATCGCCGCCATGTGCATCCAC
AGCCTGCCCGAGTACCAGGCCCGCGAGGCGGCGGCCGCCGTGGCTGCGGTGGCCGCGGGCCGCAGCCCGGAAGGCGTGCG
CGACGACCCGGTGCTGCGACGCCTCGAGTACTTCTGCATCGCCTGGTTCAGCTTCGAGGTGTCGTCGCGCCTCCTGCTGG
CGCCCAGTACGCGCAACTTCTTCTGCCACCCGCTCAACCTCATCGACATTGTGTCTGTGCTGCCCTTCTATCTCACGCTG
CTGGCTGGTGTGGCACTGGGCGACCAGGGCGGCAAGGAGTTCGGCCACCTGGGCAAGGTGGTGCAGGTGTTCCGCCTCAT
GCGCATCTTCCGCGTACTCAAGTTGGCGCGCCATTCCACCGGGCTGCGCTCGCTGGGAGCCACGCTCAAGCACAGCTACC
GTGAGGTGGGCATCTTGCTGCTGTACCTGGCTGTGGGTGTGTCAGTGTTCTCTGGTGTGGCCTACACAGCTGAAAAGGAG
GAGGACGTGGGCTTTAACACCATCCCAGCCTGCTGGTGGTGGGGCACAGTGAGCATGACCACCGTGGGCTATGGGGATGT
GGTGCCAGTGACGGTGGCTGGCAAGCTGGCAGCCTCAGGCTGCATCCTAGGGGGCATCCTGGTGGTAGCACTCCCCATCA
CCATCATCTTCAACAAGTTCTCCCACTTCTACCGGCGCCAGAAGGCTCTGGAGGCAGCCGTGCGCAACAGCAACCACCAA
GAGTTTGAGGACTTGCTGAGCAGCGTTGATGGGGTGTCGGAGGCATCTCTGGAGACATCCCGAGAAACCTCTCAGGAGGG
ACAGTCTGCAGATCTAGAGAGCCAGGCCCCCAGTGAGCCTCCACACCCTCAGATGTATACAGCTTTCTTGTACAAAGTGG
TTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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