Over Expression Cells for PLLP

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

PLLP

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-PLLP-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Single Cell Clones:C1, C4, C10, E5, F10, H6,

Clones F10 and H6 have high punctate intracellular and membraneous expression of V5. V5 expression in the membrane is high than intracellular expression.

Sequence

ORF Sequence:

ATGGCCGAGTTCCCGTCGAAAGTTAGCACGCGGACCAGCAGTCCTGCGCAGGGCGCCGAAGCCTCGGTGTCGGCGCTGCG CCCGGACCTGGGCTTCGTGCGCTCCCGCCTCGGGGCGCTCATGCTGCTGCAGCTGGTGCTGGGGCTGCTGGTGTGGGCGC TGATTGCGGACACCCCGTACCACCTGTATCCGGCCTATGGCTGGGTGATGTTCGTCGCTGTCTTCCTCTGGCTGGTGACA ATCGTCCTCTTCAACCTCTACCTGTTTCAGCTGCACATGAAGTTGTACATGGTTCCCTGGCCACTGGTGTTAATGATCTT TAACATCAGCGCCACCGTTCTCTACATCACCGCCTTCATCGCCTGCTCTGCGGCAGTTGACCTGACATCCCTGAGGGGCA CCCGGCCTTATAACCAGCGCGCGGCTGCCTCGTTCTTTGCGTGTTTGGTGATGATCGCCTATGGAGTGAGTGCCTTCTTC AGCTACCAGGCCTGGCGAGGAGTAGGCAGCAATGCGGCCACCAGTCAGATGGCTGGCGGCTATGCCTACCCAACTTTCTT GTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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