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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-PLLP-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

 

Single Cell Clones: 

 

Clones E5 and H6 have high punctate intracellular and membraneous expression of V5. V5 expression in the membrane is high than intracellular expression. 

Sequence

ORF Sequence:

ATGGCCGAGTTCCCGTCGAAAGTTAGCACGCGGACCAGCAGTCCTGCGCAGGGCGCCGAAGCCTCGGTGTCGGCGCTGCG CCCGGACCTGGGCTTCGTGCGCTCCCGCCTCGGGGCGCTCATGCTGCTGCAGCTGGTGCTGGGGCTGCTGGTGTGGGCGC TGATTGCGGACACCCCGTACCACCTGTATCCGGCCTATGGCTGGGTGATGTTCGTCGCTGTCTTCCTCTGGCTGGTGACA ATCGTCCTCTTCAACCTCTACCTGTTTCAGCTGCACATGAAGTTGTACATGGTTCCCTGGCCACTGGTGTTAATGATCTT TAACATCAGCGCCACCGTTCTCTACATCACCGCCTTCATCGCCTGCTCTGCGGCAGTTGACCTGACATCCCTGAGGGGCA CCCGGCCTTATAACCAGCGCGCGGCTGCCTCGTTCTTTGCGTGTTTGGTGATGATCGCCTATGGAGTGAGTGCCTTCTTC AGCTACCAGGCCTGGCGAGGAGTAGGCAGCAATGCGGCCACCAGTCAGATGGCTGGCGGCTATGCCTACCCAACTTTCTT GTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_PLLP_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGGCCGAGTTCCCGTCGAAAGTTAGCACGCGGACCAGCAGTCCTGCGCAGGGCGCCGAAGCCTCGGTGTCGGCGCTGCG
CCCGGACCTGGGCTTCGTGCGCTCCCGCCTCGGGGCGCTCATGCTGCTGCAGCTGGTGCTGGGGCTGCTGGTGTGGGCGC
TGATTGCGGACACCCCGTACCACCTGTATCCGGCCTATGGCTGGGTGATGTTCGTCGCTGTCTTCCTCTGGCTGGTGACA
ATCGTCCTCTTCAACCTCTACCTGTTTCAGCTGCACATGAAGTTGTACATGGTTCCCTGGCCACTGGTGTTAATGATCTT
TAACATCAGCGCCACCGTTCTCTACATCACCGCCTTCATCGCCTGCTCTGCGGCAGTTGACCTGACATCCCTGAGGGGCA
CCCGGCCTTATAACCAGCGCGCGGCTGCCTCGTTCTTTGCGTGTTTGGTGATGATCGCCTATGGAGTGAGTGCCTTCTTC
AGCTACCAGGCCTGGCGAGGAGTAGGCAGCAATGCGGCCACCAGTCAGATGGCTGGCGGCTATGCCTACCCAACTTTCTT
GTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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