Over Expressed Cell Line for TMC4

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

TMC4

Cell Line Taxonomy:

293T

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-TMC4-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGAAGGGTGGGGGCGGTCAGAGGGATGTTGGCGCTGACAGGACCGTGTTTCTTCGCCTCGCCTCCCTGGTGGTCCTGCT
CTTCTCTCTCTGGAATCAGATCACTTGTGGGGGCGACTCCGAGGCTGAGGACTGCAAAACCTGTGGCTACAATTACAAAC
AACTTCCGTGCTGGGAGACTGTCCTGGGCCAGGAAATGTACAAACTTCTGCTCTTTGATCTGCTGACTGTCTTGGCAGTC
GCGCTGCTCATCCAGTTTCCTAGAAAGCTCCTCTGTGGCCTCTGTCCTGGGGCGCTGGGTCGTCTGGCGGGGACCCAGGA
GTTCCAGGTGCCCGACGAGGTGCTGGGGCTCATCTACGCGCAGACGGTGGTCTGGGTGGGGAGTTTTTTCTGCCCTTTAC
TGCCCCTGCTTAACACGGTCAAGTTCCTGCTGCTTTTCTACCTGAAGAAGCTTACCCTCTTCTCCACCTGCTCCCCGGCT
GCCCGCACCTTCCGGGCCTCCGCGGCGAATTTCTTTTTCCCCTTGGTCCTTCTCCTGGGTCTGGCCATCTCCAGCGTTCC
CCTGCTTTACAGCATCTTCCTGATCCCGCCTTCTAAGCTTTGTGGTCCATTCCGGGGGCAGTCGTCCATCTGGGCCCAGA
TCCCTGAGTCTATTTCCAGCCTCCCTGAGACCACCCAGAATTTCCTCTTCTTCCTGGGGACCCAGGCTTTTGCTGTGCCC
CTTCTGCTGATCTCCAGCATCCTGATGGCGTACACTGTGGCTCTGGCTAACTCCTACGGACGCCTCATCTCTGAGCTCAA
ACGTCAGAGACAGACGGAGGCGCAGAATAAAGTCTTCCTGGCACGGCGCGCTGTGGCGCTGACCTCCACCAAACCGGCTC
TTTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Over Expressed Cell Line for TMC4

Basic Information

Sequence

Media and Files