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Data and Resources for TMC4 Gene

Disease Associated Data

Basic Information

Gene Taxonomy:

UniprotID:

Q7Z404

Mouse Homolog:

Tmc4

Pharos Link:

Knockout Mouse Phenotype:

Monogenic Disease Association:

Polygenic Disease Association:

platelet function tests

10K Immunome:

SARS-CoV-2 Association:

SARS-CoV-2 Vaccine Association:

Over Expression Cell Lines

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

Cell Line Taxonomy:

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-TMC4-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGAAGGGTGGGGGCGGTCAGAGGGATGTTGGCGCTGACAGGACCGTGTTTCTTCGCCTCGCCTCCCTGGTGGTCCTGCT
CTTCTCTCTCTGGAATCAGATCACTTGTGGGGGCGACTCCGAGGCTGAGGACTGCAAAACCTGTGGCTACAATTACAAAC
AACTTCCGTGCTGGGAGACTGTCCTGGGCCAGGAAATGTACAAACTTCTGCTCTTTGATCTGCTGACTGTCTTGGCAGTC
GCGCTGCTCATCCAGTTTCCTAGAAAGCTCCTCTGTGGCCTCTGTCCTGGGGCGCTGGGTCGTCTGGCGGGGACCCAGGA
GTTCCAGGTGCCCGACGAGGTGCTGGGGCTCATCTACGCGCAGACGGTGGTCTGGGTGGGGAGTTTTTTCTGCCCTTTAC
TGCCCCTGCTTAACACGGTCAAGTTCCTGCTGCTTTTCTACCTGAAGAAGCTTACCCTCTTCTCCACCTGCTCCCCGGCT
GCCCGCACCTTCCGGGCCTCCGCGGCGAATTTCTTTTTCCCCTTGGTCCTTCTCCTGGGTCTGGCCATCTCCAGCGTTCC
CCTGCTTTACAGCATCTTCCTGATCCCGCCTTCTAAGCTTTGTGGTCCATTCCGGGGGCAGTCGTCCATCTGGGCCCAGA
TCCCTGAGTCTATTTCCAGCCTCCCTGAGACCACCCAGAATTTCCTCTTCTTCCTGGGGACCCAGGCTTTTGCTGTGCCC
CTTCTGCTGATCTCCAGCATCCTGATGGCGTACACTGTGGCTCTGGCTAACTCCTACGGACGCCTCATCTCTGAGCTCAA
ACGTCAGAGACAGACGGAGGCGCAGAATAAAGTCTTCCTGGCACGGCGCGCTGTGGCGCTGACCTCCACCAAACCGGCTC
TTTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

In Situ Expression

Basic Information

Gene Taxonomy:

Injury Type:

Neuron Type:

Dorsal Root Ganglion

Tissue:

DRG

Conditions:

naive vs injury (SNI 7days)

Probe:

Probe Mm-Tmc4 #592311 At Advanced Cell Diagnostics

Species:

Mouse

Media and Files

Normal Tissue Image:

Tmc4

Expression level comparison:

Tmc4

Injured Tissue Image:

Tmc4

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:

Virus Type:

Lentiviral Vector

Vector Type:

Construct ID:

IDG_TMC4_OE_1

Selection:

Amp, Blasticidin

Vector:

lPX304

Construct Type:

Expression

Tag:

C terminal V5

Passed QC:

Yes

Sequence

Inserted_sequence:

ATGAAGGGTGGGGGCGGTCAGAGGGATGTTGGCGCTGACAGGACCGTGTTTCTTCGCCTCGCCTCCCTGGTGGTCCTGCT
CTTCTCTCTCTGGAATCAGATCACTTGTGGGGGCGACTCCGAGGCTGAGGACTGCAAAACCTGTGGCTACAATTACAAAC
AACTTCCGTGCTGGGAGACTGTCCTGGGCCAGGAAATGTACAAACTTCTGCTCTTTGATCTGCTGACTGTCTTGGCAGTC
GCGCTGCTCATCCAGTTTCCTAGAAAGCTCCTCTGTGGCCTCTGTCCTGGGGCGCTGGGTCGTCTGGCGGGGACCCAGGA
GTTCCAGGTGCCCGACGAGGTGCTGGGGCTCATCTACGCGCAGACGGTGGTCTGGGTGGGGAGTTTTTTCTGCCCTTTAC
TGCCCCTGCTTAACACGGTCAAGTTCCTGCTGCTTTTCTACCTGAAGAAGCTTACCCTCTTCTCCACCTGCTCCCCGGCT
GCCCGCACCTTCCGGGCCTCCGCGGCGAATTTCTTTTTCCCCTTGGTCCTTCTCCTGGGTCTGGCCATCTCCAGCGTTCC
CCTGCTTTACAGCATCTTCCTGATCCCGCCTTCTAAGCTTTGTGGTCCATTCCGGGGGCAGTCGTCCATCTGGGCCCAGA
TCCCTGAGTCTATTTCCAGCCTCCCTGAGACCACCCAGAATTTCCTCTTCTTCCTGGGGACCCAGGCTTTTGCTGTGCCC
CTTCTGCTGATCTCCAGCATCCTGATGGCGTACACTGTGGCTCTGGCTAACTCCTACGGACGCCTCATCTCTGAGCTCAA
ACGTCAGAGACAGACGGAGGCGCAGAATAAAGTCTTCCTGGCACGGCGCGCTGTGGCGCTGACCTCCACCAAACCGGCTC
TTTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:

D.IC38-pLX304-TMC4-expression-vector.dna

Constructs for CRISPR Interference

Basic Information

Gene Taxonomy:

Construct ID:

IDG_TMC4_UE_1

Virus Type:

Lentiviral Vector

Selection:

Puro,Amp

Vector:

783_hU6_EGFP

Passed QC:

On

Vector Type:

CRISPR Interference

Sequence

Inserted_sequence:

GCCGGAAAGCCAGAACAGGT

Media and Files

Vector Map:

Snapgene File:

H4-783-TMC4-interference-vector.dna

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