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Data and Resources for CACNA2D4 Gene

Disease Associated Data

Basic Information

Gene Taxonomy:
CACNA2D4
UniprotID:
Q7Z3S7
Mouse Homolog:
Cacna2d4
Pharos Link:
Pharos Link
Knockout Mouse Phenotype:
abnormal rod electrophysiology
abnormal eye electrophysiology
increased bone mineral content
abnormal retinal outer nuclear layer morphology
abnormal retinal blood vessel morphology
thin retinal outer plexiform layer
abnormal cone electrophysiology
abnormal retinal layer morphology
abnormal retinal inner plexiform layer morphology
abnormal retinal outer plexiform layer morphology
Monogenic Disease Association:
retinal cone dystrophy 4
Polygenic Disease Association:
bipolar disorder
cholesterol
ldl
echocardiography
10K Immunome:
Not Detected
SARS-CoV-2 Association:
Not Detected
SARS-CoV-2 Vaccine Association:
Not Detected

Over Expression Cell Lines

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
CACNA2D4
Cell Line Taxonomy:
293T
Over Expression Cell Line
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CACNA2D4-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCCCAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGTGTCTGGGGCTCCTGGTACGACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCAGGACCCGCTGCAGCCCTGCGACACGGAGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATTTGTGGTGCAGCAGATTCCCAACAGTAACCTCCTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGACAGAAGTCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCCCCTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGTCCAACTTTCTTGTACAAAGTGGTTG GTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:
CACNA2D4
Virus Type:
Lentiviral Vector
Vector Type:
cDNA
Construct ID:
IDG_CACNA2D4_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCCCAATTTCTGC
CTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGTGTCTGGGGCTCCTGGTACG
ACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCAGGACCCGCTGCAGCCCTGCGACACG
GAGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATT
TGTGGTGCAGCAGATTCCCAACAGTAACCTCCTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAG
TGCTGCAGGAGGCGACAGAAGTCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGA
CCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCC
CCTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGTCCAACTTTCTTGTACAAAGTGGTTG
GTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:
D.IC32-pLX304-CACNA2D4-expression-vector.dna

Constructs for CRISPR Interference

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Exploring the Dark Genome