10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for CHRNB1 Gene
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-CHRNB1-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ORF Sequence:
ATGACCCCAGGGGCTCTGCTGATGCTGCTGGGGGCGCTGGGGGCGCCGCTCGCCCCAGGCGTCCGCGGCTCGGAGGCGGA GGGTCGACTCCGGGAGAAACTTTTCTCTGGCTATGATAGCTCCGTGCGGCCAGCGCGGGAGGTGGGAGACCGTGTCAGGG TCAGCGTTGGTCTCATCCTGGCGCAACTCATCAGCCTGAACGAGAAGGATGAAGAGATGAGCACAAAGGTGTACTTAGAC CTGGAGTGGACTGACTACAGGCTGAGCTGGGACCCTGCGGAGCACGACGGCATCGATTCGCTCCGCATCACGGCGGAATC CGTGTGGCTCCCTGACGTGGTGCTACTGAACAACAATGATGGGAATTTTGACGTGGCTCTGGACATTAGCGTCGTGGTGT CCTCCGACGGCTCCGTGCGTTGGCAACCCCCGGGCATCTATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTC GACTGGCAGAATTGCACTATGGTGTTCAGCTCCTACAGCTACGACAGCTCGGAGGTCAGCCTGCAGACAGGCCTGGGTCC TGACGGGCAAGGGCATCAGGAAATCCACATTCATGAAGGGACTTTCATTGAGAATGGCCAGTGGGAGATTATCCACAAGC CCTCTCGGCTAATCCAGCCTCCAGGCGATCCTAGGGGAGGGAGGGAAGGACAGCGCCAGGAAGTCATCTTCTACCTCATC ATCCGCCGCAAGCCTCTCTTCTACCTGGTCAACGTCATTGCCCCATGCATCCTCATCACTCTTCTGGCCATCTTCGTCTT CTACCTGCCACCAGATGCAGGAGAGAAGATGGGGCTCTCAATCTTTGCCCTGCTGACCCTTACTGTGTTCCTGCTGCTGC TGGCTGACAAAGTACCTGAGACCTCACTATCAGTACCCATTATTATCAAGTACCTCATGTTTACCATGGTCCTCGTCACC TTCTCAGTCATCCTTAGTGTCGTGGTTCTCAACCTGCACCACCGCTCACCCCACACCCACCAAATGCCCCTTTGGGTCCG TCAGATCTTCATTCACAAACTTCCGCTGTACCTGCGTCTAAAAAGGCCCAAACCCGAGAGAGACCTGATGCCGGAGCCCC CTCACTGTTCTTCTCCAGGAAGTGGCTGGGGTCGGGGAACAGATGAATATTTCATCCGGAAGCCGCCAAGTGATTTTCTC TTCCCCAAACCCAATAGGTTCCAGCCTGAACTGTCTGCCCCTGATCTGCGGCGATTTATCGATGGTCCAAACCGGGCTGT GGCCCTGCTTCCGGAGCTACGGGAGGTCGTCTCCTCTATCAGCTACATCGCTCGACAGCTGCAGGAACAGGAGGACCACG ATGCGCTGAAGGAGGACTGGCAGTTTGTGGCCATGGTAGTGGACCGCCTCTTCCTGTGGACTTTCATCATCTTCACCAGC GTTGGGACCCTAGTCATCTTCCTGGACGCCACGTACCACTTGCCCCCTCCAGACCCCTTTCCTTGCCCAACTTTCTTGTA CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_CHRNB1_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGACCCCAGGGGCTCTGCTGATGCTGCTGGGGGCGCTGGGGGCGCCGCTCGCCCCAGGCGTCCGCGGCTCGGAGGCGGA
GGGTCGACTCCGGGAGAAACTTTTCTCTGGCTATGATAGCTCCGTGCGGCCAGCGCGGGAGGTGGGAGACCGTGTCAGGG
TCAGCGTTGGTCTCATCCTGGCGCAACTCATCAGCCTGAACGAGAAGGATGAAGAGATGAGCACAAAGGTGTACTTAGAC
CTGGAGTGGACTGACTACAGGCTGAGCTGGGACCCTGCGGAGCACGACGGCATCGATTCGCTCCGCATCACGGCGGAATC
CGTGTGGCTCCCTGACGTGGTGCTACTGAACAACAATGATGGGAATTTTGACGTGGCTCTGGACATTAGCGTCGTGGTGT
CCTCCGACGGCTCCGTGCGTTGGCAACCCCCGGGCATCTATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTC
GACTGGCAGAATTGCACTATGGTGTTCAGCTCCTACAGCTACGACAGCTCGGAGGTCAGCCTGCAGACAGGCCTGGGTCC
TGACGGGCAAGGGCATCAGGAAATCCACATTCATGAAGGGACTTTCATTGAGAATGGCCAGTGGGAGATTATCCACAAGC
CCTCTCGGCTAATCCAGCCTCCAGGCGATCCTAGGGGAGGGAGGGAAGGACAGCGCCAGGAAGTCATCTTCTACCTCATC
ATCCGCCGCAAGCCTCTCTTCTACCTGGTCAACGTCATTGCCCCATGCATCCTCATCACTCTTCTGGCCATCTTCGTCTT
CTACCTGCCACCAGATGCAGGAGAGAAGATGGGGCTCTCAATCTTTGCCCTGCTGACCCTTACTGTGTTCCTGCTGCTGC
TGGCTGACAAAGTACCTGAGACCTCACTATCAGTACCCATTATTATCAAGTACCTCATGTTTACCATGGTCCTCGTCACC
TTCTCAGTCATCCTTAGTGTCGTGGTTCTCAACCTGCACCACCGCTCACCCCACACCCACCAAATGCCCCTTTGGGTCCG
TCAGATCTTCATTCACAAACTTCCGCTGTACCTGCGTCTAAAAAGGCCCAAACCCGAGAGAGACCTGATGCCGGAGCCCC
CTCACTGTTCTTCTCCAGGAAGTGGCTGGGGTCGGGGAACAGATGAATATTTCATCCGGAAGCCGCCAAGTGATTTTCTC
TTCCCCAAACCCAATAGGTTCCAGCCTGAACTGTCTGCCCCTGATCTGCGGCGATTTATCGATGGTCCAAACCGGGCTGT
GGCCCTGCTTCCGGAGCTACGGGAGGTCGTCTCCTCTATCAGCTACATCGCTCGACAGCTGCAGGAACAGGAGGACCACG
ATGCGCTGAAGGAGGACTGGCAGTTTGTGGCCATGGTAGTGGACCGCCTCTTCCTGTGGACTTTCATCATCTTCACCAGC
GTTGGGACCCTAGTCATCTTCCTGGACGCCACGTACCACTTGCCCCCTCCAGACCCCTTTCCTTGCCCAACTTTCTTGTA
CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
GGGTCGACTCCGGGAGAAACTTTTCTCTGGCTATGATAGCTCCGTGCGGCCAGCGCGGGAGGTGGGAGACCGTGTCAGGG
TCAGCGTTGGTCTCATCCTGGCGCAACTCATCAGCCTGAACGAGAAGGATGAAGAGATGAGCACAAAGGTGTACTTAGAC
CTGGAGTGGACTGACTACAGGCTGAGCTGGGACCCTGCGGAGCACGACGGCATCGATTCGCTCCGCATCACGGCGGAATC
CGTGTGGCTCCCTGACGTGGTGCTACTGAACAACAATGATGGGAATTTTGACGTGGCTCTGGACATTAGCGTCGTGGTGT
CCTCCGACGGCTCCGTGCGTTGGCAACCCCCGGGCATCTATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTC
GACTGGCAGAATTGCACTATGGTGTTCAGCTCCTACAGCTACGACAGCTCGGAGGTCAGCCTGCAGACAGGCCTGGGTCC
TGACGGGCAAGGGCATCAGGAAATCCACATTCATGAAGGGACTTTCATTGAGAATGGCCAGTGGGAGATTATCCACAAGC
CCTCTCGGCTAATCCAGCCTCCAGGCGATCCTAGGGGAGGGAGGGAAGGACAGCGCCAGGAAGTCATCTTCTACCTCATC
ATCCGCCGCAAGCCTCTCTTCTACCTGGTCAACGTCATTGCCCCATGCATCCTCATCACTCTTCTGGCCATCTTCGTCTT
CTACCTGCCACCAGATGCAGGAGAGAAGATGGGGCTCTCAATCTTTGCCCTGCTGACCCTTACTGTGTTCCTGCTGCTGC
TGGCTGACAAAGTACCTGAGACCTCACTATCAGTACCCATTATTATCAAGTACCTCATGTTTACCATGGTCCTCGTCACC
TTCTCAGTCATCCTTAGTGTCGTGGTTCTCAACCTGCACCACCGCTCACCCCACACCCACCAAATGCCCCTTTGGGTCCG
TCAGATCTTCATTCACAAACTTCCGCTGTACCTGCGTCTAAAAAGGCCCAAACCCGAGAGAGACCTGATGCCGGAGCCCC
CTCACTGTTCTTCTCCAGGAAGTGGCTGGGGTCGGGGAACAGATGAATATTTCATCCGGAAGCCGCCAAGTGATTTTCTC
TTCCCCAAACCCAATAGGTTCCAGCCTGAACTGTCTGCCCCTGATCTGCGGCGATTTATCGATGGTCCAAACCGGGCTGT
GGCCCTGCTTCCGGAGCTACGGGAGGTCGTCTCCTCTATCAGCTACATCGCTCGACAGCTGCAGGAACAGGAGGACCACG
ATGCGCTGAAGGAGGACTGGCAGTTTGTGGCCATGGTAGTGGACCGCCTCTTCCTGTGGACTTTCATCATCTTCACCAGC
GTTGGGACCCTAGTCATCTTCCTGGACGCCACGTACCACTTGCCCCCTCCAGACCCCTTTCCTTGCCCAACTTTCTTGTA
CAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Snapgene File:
Basic Information
Gene Taxonomy:
Construct ID:
IDG_CHRNB1_UE_1
Virus Type:
Selection:
Puro,Amp
Vector:
783_hU6_EGFP
Passed QC:
On
Vector Type:
Sequence
Inserted_sequence:
GGGCAGGGCCATGGCCTTAG
Media and Files
Vector Map:
Snapgene File: