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Data and Resources for CLIC5 Gene

Disease Associated Data

Basic Information

Gene Taxonomy:

UniprotID:

Q9NZA1

Mouse Homolog:

Clic5

Pharos Link:

Knockout Mouse Phenotype:

decreased body size

abnormal renal glomerulus morphology

Gait disturbance

Abnormal renal physiology

decreased circulating glucose level

Adipose tissue loss

Hypoinsulinemia

Decreased serum leptin

Hearing impairment

Gastrointestinal hemorrhage

decreased bone mineral content

decreased prepulse inhibition

decreased body length

increased or absent threshold for auditory brainstem response

decreased eosinophil cell number

decreased startle reflex

increased respiratory quotient

absent startle reflex

abnormal pulmonary alveolus morphology

abnormal lens development

vestibular hair cell degeneration

increased susceptibility to weight loss

cochlear ganglion degeneration

podocyte foot process effacement

cochlear outer hair cell degeneration

decreased susceptibility to hepatic steatosis

organ of Corti degeneration

increased susceptibility to injury

bidirectional circling

impaired swimming

abnormal cochlear hair cell stereociliary bundle morphology

increased circulating glucagon level

increased stomach pH

cochlear inner hair cell degeneration

increased aggression towards males

abnormal glomerular capillary endothelium morphology

abnormal podocyte foot process morphology

decreased digestive secretion

abnormal glomerular endothelium fenestra morphology

Monogenic Disease Association:

Polygenic Disease Association:

leukocyte count

10K Immunome:

SARS-CoV-2 Association:

SARS-CoV-2 Vaccine Association:

Over Expression Cell Lines

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

Cell Line Taxonomy:

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-CLIC5-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGACAGACTCGGCGACAGCTAACGGGGACGACAGGGACCCCGAGATCGAGCTCTTTGTGAAGGCTGGAATCGATGGAGA
AAGCATCGGCAACTGTCCTTTCTCTCAGCGCCTCTTCATGATCCTCTGGCTGAAAGGAGTCGTGTTCAATGTCACCACTG
TGGATCTGAAAAGAAAGCCAGCTGACCTGCACAACCTAGCCCCCGGCACGCACCCGCCCTTCCTGACCTTCAACGGGGAC
GTGAAGACAGACGTCAATAAGATCGAGGAGTTCCTGGAGGAGACCTTGACCCCTGAAAAGTACCCCAAACTGGCTGCAAA
ACACCGGGAATCCAACACAGCGGGCATCGACATCTTTTCCAAGTTTTCTGCCTACATCAAAAATACCAAGCAGCAGAACA
ATGCTGCTCTTGAAAGAGGCCTAACCAAGGCTCTAAAGAAATTGGATGACTACCTGAACACCCCTCTACCAGAGGAGATT
GACGCCAACACTTGTGGGGAAGACAAGGGGTCCCGGCGCAAGTTCCTGGATGGGGATGAGCTGACCCTGGCTGACTGCAA
TCTGTTGCCCAAGCTCCATGTGGTCAAGATTGTGGCCAAGAAATACCGCAACTATGATATCCCGGCTGAGATGACAGGCC
TGTGGCGGTACCTCAAGAACGCCTATGCCCGTGATGAGTTCACCAACACCTGTGCAGCTGACAGTGAGATCGAGTTGGCC
TACGCTGATGTCGCCAAACGCCTCAGCCGATCCTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:

Virus Type:

Lentiviral Vector

Vector Type:

Construct ID:

IDG_CLIC5_OE_1

Selection:

Amp, Blasticidin

Vector:

lPX304

Construct Type:

Expression

Tag:

C terminal V5

Passed QC:

Yes

Sequence

Inserted_sequence:

ATGACAGACTCGGCGACAGCTAACGGGGACGACAGGGACCCCGAGATCGAGCTCTTTGTGAAGGCTGGAATCGATGGAGA
AAGCATCGGCAACTGTCCTTTCTCTCAGCGCCTCTTCATGATCCTCTGGCTGAAAGGAGTCGTGTTCAATGTCACCACTG
TGGATCTGAAAAGAAAGCCAGCTGACCTGCACAACCTAGCCCCCGGCACGCACCCGCCCTTCCTGACCTTCAACGGGGAC
GTGAAGACAGACGTCAATAAGATCGAGGAGTTCCTGGAGGAGACCTTGACCCCTGAAAAGTACCCCAAACTGGCTGCAAA
ACACCGGGAATCCAACACAGCGGGCATCGACATCTTTTCCAAGTTTTCTGCCTACATCAAAAATACCAAGCAGCAGAACA
ATGCTGCTCTTGAAAGAGGCCTAACCAAGGCTCTAAAGAAATTGGATGACTACCTGAACACCCCTCTACCAGAGGAGATT
GACGCCAACACTTGTGGGGAAGACAAGGGGTCCCGGCGCAAGTTCCTGGATGGGGATGAGCTGACCCTGGCTGACTGCAA
TCTGTTGCCCAAGCTCCATGTGGTCAAGATTGTGGCCAAGAAATACCGCAACTATGATATCCCGGCTGAGATGACAGGCC
TGTGGCGGTACCTCAAGAACGCCTATGCCCGTGATGAGTTCACCAACACCTGTGCAGCTGACAGTGAGATCGAGTTGGCC
TACGCTGATGTCGCCAAACGCCTCAGCCGATCCTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:

D.IC27-pLX304-CLIC5-expression-vector.dna

Constructs for CRISPR Interference

Basic Information

Gene Taxonomy:

Construct ID:

IDG_CLIC5_UE_1

Virus Type:

Lentiviral Vector

Selection:

Puro,Amp

Vector:

783_hU6_EGFP

Passed QC:

On

Vector Type:

CRISPR Interference

Sequence

Inserted_sequence:

GCAAGTGCCTGACTGCTGGG

Media and Files

Vector Map:

Snapgene File:

C3-783-CLIC5-interference-vector.dna

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