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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-GABRG1-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGGTCCTTTGAAAGCTTTTCTCTTCTCCCCTTTTCTTCTGCGGAGTCAAAGTAGAGGGGTGAGGTTGGTCTTCTTGTT
ACTGACCCTGCATTTGGGAAACTGTGTTGATAAGGCAGATGATGAAGATGATGAGGATTTAACGGTGAACAAAACCTGGG
TCTTGGCCCCAAAAATTCATGAAGGAGATATCACACAAATTCTGAATTCATTGCTTCAAGGCTATGACAATAAACTTCGT
CCAGATATAGGAGTGAGGCCCACAGTAATTGAAACTGATGTTTATGTAAACAGCATTGGACCAGTTGATCCAATTAATAT
GGAATATACAATAGATATAATTTTTGCCCAAACCTGGTTTGACAGTCGTTTAAAATTCAATAGTACCATGAAAGTGCTTA
TGCTTAACAGTAATATGGTTGGAAAAATTTGGATTCCTGACACTTTCTTCAGAAACTCAAGAAAATCTGATGCTCACTGG
ATAACAACTCCTAATCGTCTGCTTCGAATTTGGAATGATGGACGAGTTCTGTATACTCTAAGATTGACAATTAATGCAGA
ATGTTATCTTCAGCTTCATAACTTTCCCATGGATGAACATTCCTGTCCACTGGAATTTTCAAGCTATGGATACCCTAAAA
ATGAAATTGAGTATAAGTGGAAAAAGCCCTCCGTAGAAGTGGCTGATCCTAAATACTGGAGATTATATCAGTTTGCATTT
GTAGGGTTACGGAACTCAACTGAAATCACTCACACGATCTCTGGGGATTATGTTATCATGACAATTTTTTTTGACCTGAG
CAGAAGAATGGGATATTTCACTATTCAGACCTACATTCCATGCATTCTGACAGTTGTTCTTTCTTGGGTGTCTTTTTGGA
TCAATAAAGATGCAGTGCCTGCAAGAACATCGTTGGGTATCACTACAGTTCTGACTATGACAACCCTGAGTACAATTGCC
AGGAAGTCTTTACCTAAGGTTTCTTATGTGACTGCGATGGATCTCTTTGTTTCTGTTTGTTTCATTTTTGTTTTTGCAGC
CTTGATGGAATATGGAACCTTGCATTATTTTACCAGCAACCAAAAAGGAAAGACTGCTACTAAAGACAGAAAGCTAAAAA
ATAAAGCCTCGATGACTCCTGGTCTCCATCCTGGATCCACTCTGATTCCAATGAATAATATTTCTGTGCCGCAAGAAGAT
GATTATGAGTATCAGTGTTTGGAGGGCAAAGATTGTGCCAGCTTCTTCTGTTGCTTTGAAGACTGCAGAACAGGATCTTG
GAGGGAAGGAAGGATACACATACGCATTGCCAAAATTGACTCTTATTCTAGAATATTTTTCCCAACCGCTTTTGCCCTGT
TCAACTTGGTTTATTGGGTTGGCTATCTTTACTTAACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCT
CTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_GABRG1_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGGGTCCTTTGAAAGCTTTTCTCTTCTCCCCTTTTCTTCTGCGGAGTCAAAGTAGAGGGGTGAGGTTGGTCTTCTTGTT
ACTGACCCTGCATTTGGGAAACTGTGTTGATAAGGCAGATGATGAAGATGATGAGGATTTAACGGTGAACAAAACCTGGG
TCTTGGCCCCAAAAATTCATGAAGGAGATATCACACAAATTCTGAATTCATTGCTTCAAGGCTATGACAATAAACTTCGT
CCAGATATAGGAGTGAGGCCCACAGTAATTGAAACTGATGTTTATGTAAACAGCATTGGACCAGTTGATCCAATTAATAT
GGAATATACAATAGATATAATTTTTGCCCAAACCTGGTTTGACAGTCGTTTAAAATTCAATAGTACCATGAAAGTGCTTA
TGCTTAACAGTAATATGGTTGGAAAAATTTGGATTCCTGACACTTTCTTCAGAAACTCAAGAAAATCTGATGCTCACTGG
ATAACAACTCCTAATCGTCTGCTTCGAATTTGGAATGATGGACGAGTTCTGTATACTCTAAGATTGACAATTAATGCAGA
ATGTTATCTTCAGCTTCATAACTTTCCCATGGATGAACATTCCTGTCCACTGGAATTTTCAAGCTATGGATACCCTAAAA
ATGAAATTGAGTATAAGTGGAAAAAGCCCTCCGTAGAAGTGGCTGATCCTAAATACTGGAGATTATATCAGTTTGCATTT
GTAGGGTTACGGAACTCAACTGAAATCACTCACACGATCTCTGGGGATTATGTTATCATGACAATTTTTTTTGACCTGAG
CAGAAGAATGGGATATTTCACTATTCAGACCTACATTCCATGCATTCTGACAGTTGTTCTTTCTTGGGTGTCTTTTTGGA
TCAATAAAGATGCAGTGCCTGCAAGAACATCGTTGGGTATCACTACAGTTCTGACTATGACAACCCTGAGTACAATTGCC
AGGAAGTCTTTACCTAAGGTTTCTTATGTGACTGCGATGGATCTCTTTGTTTCTGTTTGTTTCATTTTTGTTTTTGCAGC
CTTGATGGAATATGGAACCTTGCATTATTTTACCAGCAACCAAAAAGGAAAGACTGCTACTAAAGACAGAAAGCTAAAAA
ATAAAGCCTCGATGACTCCTGGTCTCCATCCTGGATCCACTCTGATTCCAATGAATAATATTTCTGTGCCGCAAGAAGAT
GATTATGAGTATCAGTGTTTGGAGGGCAAAGATTGTGCCAGCTTCTTCTGTTGCTTTGAAGACTGCAGAACAGGATCTTG
GAGGGAAGGAAGGATACACATACGCATTGCCAAAATTGACTCTTATTCTAGAATATTTTTCCCAACCGCTTTTGCCCTGT
TCAACTTGGTTTATTGGGTTGGCTATCTTTACTTAACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCT
CTCCTCGGTCTCGATTCTACGTAG

Media and Files