10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for HTR3E Gene
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-HTR3E-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Single Cell Clones:
E3, F4, F6. Majority of cells are expressing V5 at very low intensity.
Sequence
ORF Sequence:
atgtctgccatcctagatgtgaatgaacagctgcacctcttgtcatcattcctgtggctggaaatggtttgggataaccc atttatcagctggaacccagaggaatgtgagggcatcacgaagatgagtatggcagccaagaacctgtggctcccagaca ttttcatcattgaactcatggatgtggataagaccccaaaaggcctcacagcatatgtaagtaatgaaggtcgcatcagg tataagaaacccatgaaggtggacagtatctgtaacctggacatcttctacttccccttcgaccagcagaactgcacact caccttcagctcattcctctacacagtggacagcatgttgctggacatggagaaagaagtgtgggaaataacagacgcat cccggaacatccttcagacccatggagaatgggagctcctgggcctcagcaaggccaccgcaaagttgtccagggtggcc atcaggcgcaggcccagcctctatgtcataaaccttctcgtgcccagtggctttctggttgccatcgatgccctcagctt ctacctgccagtgaaaagtgggaatcgtgtcccattcaagataacgctcctgctgggctacaacgtcttcctgctcatga tgagtgacttgctccccaccagtggcacccccctcatcggtgtctacttcgccctgtgcctgtccctgatggtgggcagc ctgctggagaccatcttcatcacccacctgctgcacgtggccaccacccagcccccacccctgcctcggtggctccactc cctgctgctccactgcaacagcccggggagatgctgtcccactgcgccccagaaggaaaataagggcccgggtctcaccc ccacccacctgcccggtgtgaaggagccagaggtatcagcagggcagatgccgggccctgcggaggcagagctgacaggg ggctcagaatggacaagggcccagcgggaacacgaggcccagaagcagcactcagtggagctgtggttgcagttcagcca cgcgatggacgccatgctcttccgcctctacctgctcttcatggcctcctctatcatcaccgtcatatgcctctggaaca ccTTGCCAACTTTCTTGTACAAAGTGGTtggtaagcctatccctaaccctctcctcggtctcgattctacgtag
Media and Files
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_HTR3E_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGTCTGCCATCCTAGATGTGAATGAACAGCTGCACCTCTTGTCATCATTCCTGTGGCTGGAAATGGTTTGGGATAACCC
ATTTATCAGCTGGAACCCAGAGGAATGTGAGGGCATCACGAAGATGAGTATGGCAGCCAAGAACCTGTGGCTCCCAGACA
TTTTCATCATTGAACTCATGGATGTGGATAAGACCCCAAAAGGCCTCACAGCATATGTAAGTAATGAAGGTCGCATCAGG
TATAAGAAACCCATGAAGGTGGACAGTATCTGTAACCTGGACATCTTCTACTTCCCCTTCGACCAGCAGAACTGCACACT
CACCTTCAGCTCATTCCTCTACACAGTGGACAGCATGTTGCTGGACATGGAGAAAGAAGTGTGGGAAATAACAGACGCAT
CCCGGAACATCCTTCAGACCCATGGAGAATGGGAGCTCCTGGGCCTCAGCAAGGCCACCGCAAAGTTGTCCAGGGTGGCC
ATCAGGCGCAGGCCCAGCCTCTATGTCATAAACCTTCTCGTGCCCAGTGGCTTTCTGGTTGCCATCGATGCCCTCAGCTT
CTACCTGCCAGTGAAAAGTGGGAATCGTGTCCCATTCAAGATAACGCTCCTGCTGGGCTACAACGTCTTCCTGCTCATGA
TGAGTGACTTGCTCCCCACCAGTGGCACCCCCCTCATCGGTGTCTACTTCGCCCTGTGCCTGTCCCTGATGGTGGGCAGC
CTGCTGGAGACCATCTTCATCACCCACCTGCTGCACGTGGCCACCACCCAGCCCCCACCCCTGCCTCGGTGGCTCCACTC
CCTGCTGCTCCACTGCAACAGCCCGGGGAGATGCTGTCCCACTGCGCCCCAGAAGGAAAATAAGGGCCCGGGTCTCACCC
CCACCCACCTGCCCGGTGTGAAGGAGCCAGAGGTATCAGCAGGGCAGATGCCGGGCCCTGCGGAGGCAGAGCTGACAGGG
GGCTCAGAATGGACAAGGGCCCAGCGGGAACACGAGGCCCAGAAGCAGCACTCAGTGGAGCTGTGGTTGCAGTTCAGCCA
CGCGATGGACGCCATGCTCTTCCGCCTCTACCTGCTCTTCATGGCCTCCTCTATCATCACCGTCATATGCCTCTGGAACA
CCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
ATTTATCAGCTGGAACCCAGAGGAATGTGAGGGCATCACGAAGATGAGTATGGCAGCCAAGAACCTGTGGCTCCCAGACA
TTTTCATCATTGAACTCATGGATGTGGATAAGACCCCAAAAGGCCTCACAGCATATGTAAGTAATGAAGGTCGCATCAGG
TATAAGAAACCCATGAAGGTGGACAGTATCTGTAACCTGGACATCTTCTACTTCCCCTTCGACCAGCAGAACTGCACACT
CACCTTCAGCTCATTCCTCTACACAGTGGACAGCATGTTGCTGGACATGGAGAAAGAAGTGTGGGAAATAACAGACGCAT
CCCGGAACATCCTTCAGACCCATGGAGAATGGGAGCTCCTGGGCCTCAGCAAGGCCACCGCAAAGTTGTCCAGGGTGGCC
ATCAGGCGCAGGCCCAGCCTCTATGTCATAAACCTTCTCGTGCCCAGTGGCTTTCTGGTTGCCATCGATGCCCTCAGCTT
CTACCTGCCAGTGAAAAGTGGGAATCGTGTCCCATTCAAGATAACGCTCCTGCTGGGCTACAACGTCTTCCTGCTCATGA
TGAGTGACTTGCTCCCCACCAGTGGCACCCCCCTCATCGGTGTCTACTTCGCCCTGTGCCTGTCCCTGATGGTGGGCAGC
CTGCTGGAGACCATCTTCATCACCCACCTGCTGCACGTGGCCACCACCCAGCCCCCACCCCTGCCTCGGTGGCTCCACTC
CCTGCTGCTCCACTGCAACAGCCCGGGGAGATGCTGTCCCACTGCGCCCCAGAAGGAAAATAAGGGCCCGGGTCTCACCC
CCACCCACCTGCCCGGTGTGAAGGAGCCAGAGGTATCAGCAGGGCAGATGCCGGGCCCTGCGGAGGCAGAGCTGACAGGG
GGCTCAGAATGGACAAGGGCCCAGCGGGAACACGAGGCCCAGAAGCAGCACTCAGTGGAGCTGTGGTTGCAGTTCAGCCA
CGCGATGGACGCCATGCTCTTCCGCCTCTACCTGCTCTTCATGGCCTCCTCTATCATCACCGTCATATGCCTCTGGAACA
CCTTGCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Snapgene File: