KCNAB2 | Dark Matter

Loading...

Disease Associated Data

Basic Information

Gene Taxonomy:

UniprotID:

Q13303

Mouse Homolog:

Kcnab2

Pharos Link:

Knockout Mouse Phenotype:

premature death

decreased body size

behavior/neurological phenotype

impaired adaptive thermogenesis

abnormal learning/memory/conditioning

abnormal spatial learning

Abnormal nervous system electrophysiology

no abnormal phenotype detected

hearing/vestibular/ear phenotype

impaired contextual conditioning behavior

sporadic seizures

decreased core body temperature

abnormal afterhyperpolarization

Monogenic Disease Association:

Polygenic Disease Association:

chromosome 1p36 deletion syndrome

10K Immunome:

SARS-CoV-2 Association:

SARS-CoV-2 Vaccine Association:

Over Expression Cell Lines

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

Cell Line Taxonomy:

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-KCNAB2-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGTATCCAGAATCAACGACGGGCTCCCCGGCTCGGCTCTCGCTGCGGCAGACGGGCTCCCCCGGGATGATCTACAGTAC
TCGGTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAACCTGGGCAAGTCTGGCCTGCGGGTCTCCTGCCTGGGAC
TTGGAACATGGGTGACCTTCGGAGGCCAGATCACCGATGAGATGGCAGAGCAGCTCATGACCTTGGCCTATGATAATGGC
ATCAACCTCTTCGATACAGCAGAAGTCTACGCAGCCGGCAAGGCTGAAGTGGTACTGGGAAACATCATTAAGAAGAAAGG
ATGGAGGCGGTCCAGCCTCGTCATCACCACCAAGATCTTCTGGGGCGGAAAGGCGGAGACGGAGCGGGGCCTGTCCAGGA
AGCACATAATCGAAGGTCTGAAAGCTTCCCTGGAGCGACTGCAGCTGGAGTACGTGGATGTGGTGTTTGCCAACCGCCCG
GACCCCAACACCCCGATGGAAGAGACCGTCCGCGCCATGACCCACGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC
GTCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCCGTGGCCCGGCAGTTCAACCTGACCCCGCCCATCTGCGAGC
AGGCTGAGTACCACATGTTCCAGCGTGAGAAAGTGGAGGTGCAGCTGCCGGAGCTGTTCCACAAGATAGGAGTGGGCGCC
ATGACCTGGTCCCCTCTGGCCTGTGGCATTGTTTCTGGCAAGTACGACAGTGGCATCCCACCCTACTCAAGAGCCTCCTT
GAAGGGCTACCAGTGGCTGAAGGACAAGATCCTCAGTGAGGAGGGCCGGCGCCAGCAAGCCAAGCTGAAGGAGCTGCAGG
CCATCGCCGAGCGCCTGGGCTGCACCCTGCCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGAGTCAGCTCCGTG
CTCCTGGGGGCCTCCAATGCGGACCAGCTCATGGAGAACATTGGGGCAATACAGGTCCTTCCGAAACTGTCGTCTTCCAT
TATCCACGAGATTGATAGTATTTTGGGCAATAAACCCTACAGCAAAAAGGACTACAGATCCTTGCCAACTTTCTTGTACA
AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

In Situ Expression

Basic Information

Gene Taxonomy:

Injury Type:

Neuron Type:

Dorsal Root Ganglion

Tissue:

DRG

Conditions:

naive vs injury (SNI 7days)

Probe:

Probe Mm-Kcnab2 #469381 At Advanced Cell Diagnostics

Species:

Mouse

Media and Files

Normal Tissue Image:

Kcnab2

Expression level comparison:

Kcnab2

Injured Tissue Image:

Kcnab2

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:

Virus Type:

Lentiviral Vector

Vector Type:

Construct ID:

IDG_KCNAB2_OE_1

Selection:

Amp, Blasticidin

Vector:

lPX304

Construct Type:

Expression

Tag:

C terminal V5

Passed QC:

Yes

Sequence

Inserted_sequence:

ATGTATCCAGAATCAACGACGGGCTCCCCGGCTCGGCTCTCGCTGCGGCAGACGGGCTCCCCCGGGATGATCTACAGTAC
TCGGTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAACCTGGGCAAGTCTGGCCTGCGGGTCTCCTGCCTGGGAC
TTGGAACATGGGTGACCTTCGGAGGCCAGATCACCGATGAGATGGCAGAGCAGCTCATGACCTTGGCCTATGATAATGGC
ATCAACCTCTTCGATACAGCAGAAGTCTACGCAGCCGGCAAGGCTGAAGTGGTACTGGGAAACATCATTAAGAAGAAAGG
ATGGAGGCGGTCCAGCCTCGTCATCACCACCAAGATCTTCTGGGGCGGAAAGGCGGAGACGGAGCGGGGCCTGTCCAGGA
AGCACATAATCGAAGGTCTGAAAGCTTCCCTGGAGCGACTGCAGCTGGAGTACGTGGATGTGGTGTTTGCCAACCGCCCG
GACCCCAACACCCCGATGGAAGAGACCGTCCGCGCCATGACCCACGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC
GTCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCCGTGGCCCGGCAGTTCAACCTGACCCCGCCCATCTGCGAGC
AGGCTGAGTACCACATGTTCCAGCGTGAGAAAGTGGAGGTGCAGCTGCCGGAGCTGTTCCACAAGATAGGAGTGGGCGCC
ATGACCTGGTCCCCTCTGGCCTGTGGCATTGTTTCTGGCAAGTACGACAGTGGCATCCCACCCTACTCAAGAGCCTCCTT
GAAGGGCTACCAGTGGCTGAAGGACAAGATCCTCAGTGAGGAGGGCCGGCGCCAGCAAGCCAAGCTGAAGGAGCTGCAGG
CCATCGCCGAGCGCCTGGGCTGCACCCTGCCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGAGTCAGCTCCGTG
CTCCTGGGGGCCTCCAATGCGGACCAGCTCATGGAGAACATTGGGGCAATACAGGTCCTTCCGAAACTGTCGTCTTCCAT
TATCCACGAGATTGATAGTATTTTGGGCAATAAACCCTACAGCAAAAAGGACTACAGATCCTTGCCAACTTTCTTGTACA
AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:

D.IC33-pLX304-KCNAB2-expression-vector.dna

Constructs for CRISPR Interference

Basic Information

Gene Taxonomy:

Construct ID:

IDG_KCNAB2_UE_1

Virus Type:

Lentiviral Vector

Selection:

Puro,Amp

Vector:

783_hU6_EGFP

Passed QC:

On

Vector Type:

CRISPR Interference

Sequence

Inserted_sequence:

GCGGAGCGAGCGGGATTCGG

Media and Files

Vector Map:

Snapgene File:

D11-783-KCNAB2-interference-vector.dna

Subscribe to KCNAB2