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Data and Resources for KCNAB2 Gene

Disease Associated Data

Basic Information

Gene Taxonomy:
KCNAB2
UniprotID:
Q13303
Mouse Homolog:
Kcnab2
Pharos Link:
Pharos Link
Knockout Mouse Phenotype:
premature death
decreased body size
behavior/neurological phenotype
impaired adaptive thermogenesis
abnormal learning/memory/conditioning
Tremor
abnormal spatial learning
Abnormal nervous system electrophysiology
no abnormal phenotype detected
hearing/vestibular/ear phenotype
impaired contextual conditioning behavior
sporadic seizures
decreased core body temperature
abnormal afterhyperpolarization
Monogenic Disease Association:
Not Detected
Polygenic Disease Association:
epilepsy
chromosome 1p36 deletion syndrome
10K Immunome:
Not Detected
SARS-CoV-2 Association:
Not Detected
SARS-CoV-2 Vaccine Association:
Not Detected

Over Expression Cell Lines

Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
KCNAB2
Cell Line Taxonomy:
293T
Over Expression Cell Line
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNAB2-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

 

Single cell clones: A5, G8

Subcellular localization: Membrane + Cytoplasm

Sequence

ORF Sequence:

ATGTATCCAGAATCAACGACGGGCTCCCCGGCTCGGCTCTCGCTGCGGCAGACGGGCTCCCCCGGGATGATCTACAGTAC TCGGTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAACCTGGGCAAGTCTGGCCTGCGGGTCTCCTGCCTGGGAC TTGGAACATGGGTGACCTTCGGAGGCCAGATCACCGATGAGATGGCAGAGCAGCTCATGACCTTGGCCTATGATAATGGC ATCAACCTCTTCGATACAGCAGAAGTCTACGCAGCCGGCAAGGCTGAAGTGGTACTGGGAAACATCATTAAGAAGAAAGG ATGGAGGCGGTCCAGCCTCGTCATCACCACCAAGATCTTCTGGGGCGGAAAGGCGGAGACGGAGCGGGGCCTGTCCAGGA AGCACATAATCGAAGGTCTGAAAGCTTCCCTGGAGCGACTGCAGCTGGAGTACGTGGATGTGGTGTTTGCCAACCGCCCG GACCCCAACACCCCGATGGAAGAGACCGTCCGCGCCATGACCCACGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC GTCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCCGTGGCCCGGCAGTTCAACCTGACCCCGCCCATCTGCGAGC AGGCTGAGTACCACATGTTCCAGCGTGAGAAAGTGGAGGTGCAGCTGCCGGAGCTGTTCCACAAGATAGGAGTGGGCGCC ATGACCTGGTCCCCTCTGGCCTGTGGCATTGTTTCTGGCAAGTACGACAGTGGCATCCCACCCTACTCAAGAGCCTCCTT GAAGGGCTACCAGTGGCTGAAGGACAAGATCCTCAGTGAGGAGGGCCGGCGCCAGCAAGCCAAGCTGAAGGAGCTGCAGG CCATCGCCGAGCGCCTGGGCTGCACCCTGCCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGAGTCAGCTCCGTG CTCCTGGGGGCCTCCAATGCGGACCAGCTCATGGAGAACATTGGGGCAATACAGGTCCTTCCGAAACTGTCGTCTTCCAT TATCCACGAGATTGATAGTATTTTGGGCAATAAACCCTACAGCAAAAAGGACTACAGATCCTTGCCAACTTTCTTGTACA AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

63X image of clone G8

In Situ Expression

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:
KCNAB2
Virus Type:
Lentiviral Vector
Vector Type:
cDNA
Construct ID:
IDG_KCNAB2_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGTATCCAGAATCAACGACGGGCTCCCCGGCTCGGCTCTCGCTGCGGCAGACGGGCTCCCCCGGGATGATCTACAGTAC
TCGGTATGGGAGTCCCAAAAGACAGCTCCAGTTTTACAGGAACCTGGGCAAGTCTGGCCTGCGGGTCTCCTGCCTGGGAC
TTGGAACATGGGTGACCTTCGGAGGCCAGATCACCGATGAGATGGCAGAGCAGCTCATGACCTTGGCCTATGATAATGGC
ATCAACCTCTTCGATACAGCAGAAGTCTACGCAGCCGGCAAGGCTGAAGTGGTACTGGGAAACATCATTAAGAAGAAAGG
ATGGAGGCGGTCCAGCCTCGTCATCACCACCAAGATCTTCTGGGGCGGAAAGGCGGAGACGGAGCGGGGCCTGTCCAGGA
AGCACATAATCGAAGGTCTGAAAGCTTCCCTGGAGCGACTGCAGCTGGAGTACGTGGATGTGGTGTTTGCCAACCGCCCG
GACCCCAACACCCCGATGGAAGAGACCGTCCGCGCCATGACCCACGTCATCAACCAGGGGATGGCCATGTACTGGGGCAC
GTCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCCGTGGCCCGGCAGTTCAACCTGACCCCGCCCATCTGCGAGC
AGGCTGAGTACCACATGTTCCAGCGTGAGAAAGTGGAGGTGCAGCTGCCGGAGCTGTTCCACAAGATAGGAGTGGGCGCC
ATGACCTGGTCCCCTCTGGCCTGTGGCATTGTTTCTGGCAAGTACGACAGTGGCATCCCACCCTACTCAAGAGCCTCCTT
GAAGGGCTACCAGTGGCTGAAGGACAAGATCCTCAGTGAGGAGGGCCGGCGCCAGCAAGCCAAGCTGAAGGAGCTGCAGG
CCATCGCCGAGCGCCTGGGCTGCACCCTGCCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGAGTCAGCTCCGTG
CTCCTGGGGGCCTCCAATGCGGACCAGCTCATGGAGAACATTGGGGCAATACAGGTCCTTCCGAAACTGTCGTCTTCCAT
TATCCACGAGATTGATAGTATTTTGGGCAATAAACCCTACAGCAAAAAGGACTACAGATCCTTGCCAACTTTCTTGTACA
AAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:
D.IC33-pLX304-KCNAB2-expression-vector.dna

Constructs for CRISPR Interference

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Exploring the Dark Genome