10K Immunome:
SARS-CoV-2 Association:
SARS-CoV-2 Vaccine Association:
Data and Resources for KCNK12 Gene
Basic Information
Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNK12-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:
This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
Sequence
ORF Sequence:
atgtcctcccgcagcccccggcccccgccccgccgtagccgccgccgcctgccgcgcccctcctgctgctgctgctgctg
ccgccgttcgcacctcaacgaggacaccggccgcttcgtgctgctggcggcgctcatcggcctctacctggtggcgggtg
ccacagtcttctcggcgctcgagagccccggcgaggcggaggcgcgggcgcgctggggcgccacgctgcgcaacttcagc
gctgcgcacggcgtggccgagccagagctgcgcgccttcctccggcactacgaggccgcgctggccgccggcgtccgcgc
cgacgcgctgcgcccgcgctgggacttccccggcgccttctacttcgtgggcaccgtggtgtcaaccataggtttcggca
tgaccacccccgcgacggtgggcgggaaggccttcctcatcgcctacgggctgttcggctgcgctgggaccatcctgttc
ttcaacctcttcctggagcgcatcatctcgctgctggccttcatcatgcgcgcctgccgggagcgccagctgcgccgcag
cggcctgctgcccgccaccttccgccgcggctccgcgctctcggaggccgacagcctggcgggctggaagccctcggtgt
accacgtgctgctcatcctgggcctgttcgccgtgctgctgtcctgctgcgcctcggccatgtacaccagcgtggagggc
tgggactacgtggactcgctctacttctgcttcgtcaccttcagcaccatcggcttcggggacctggtgagcagccagca
cgccgcctaccggaaccaggggctctaccgcctgggcaacttcctcttcatcctgctcggcgtgtgctgcatttactcgc
tcttcaacgtcatctccatcctcatcaagcaggtgctcaactggatgctgcgcaagctgagctgccgctgctgcgcgcgc
tgctgcccggctcctggcgcgcccctggcccggcgcaatgccatcaccccaggctcccggctgcgccgccgcctggccgc
gctcggcgccgaccccgcggcccgcgacagcgacgccgagggccgccgcctctcgggcgagctcatctccatgcgcgacc
tcacggcctccaacaaggtgtcgctggcgctgctgcagaagcagctgtcggagacggccaacggctacccgcgcagcgtg
tgcgtcaacacgcgccagaacggcttctcgggcggcgtgggcgcgctgggcatcatgaacaaccggctggccgagaccag
cgcctccaggACAGCTTTCTTGTACAAAGTGGTtggtaagcctatccctaaccctctcctcggtctcgattctacgtag
Media and Files
Basic Information
Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNK12_OE_1
Selection:
Amp, Blasticidin
Vector:
lPX304
Construct Type:
Expression
Tag:
C terminal V5
Passed QC:
Yes
Sequence
Inserted_sequence:
ATGTCCTCCCGCAGCCCCCGGCCCCCGCCCCGCCGTAGCCGCCGCCGCCTGCCGCGCCCCTCCTGCTGCTGCTGCTGCTG
CCGCCGTTCGCACCTCAACGAGGACACCGGCCGCTTCGTGCTGCTGGCGGCGCTCATCGGCCTCTACCTGGTGGCGGGTG
CCACAGTCTTCTCGGCGCTCGAGAGCCCCGGCGAGGCGGAGGCGCGGGCGCGCTGGGGCGCCACGCTGCGCAACTTCAGC
GCTGCGCACGGCGTGGCCGAGCCAGAGCTGCGCGCCTTCCTCCGGCACTACGAGGCCGCGCTGGCCGCCGGCGTCCGCGC
CGACGCGCTGCGCCCGCGCTGGGACTTCCCCGGCGCCTTCTACTTCGTGGGCACCGTGGTGTCAACCATAGGTTTCGGCA
TGACCACCCCCGCGACGGTGGGCGGGAAGGCCTTCCTCATCGCCTACGGGCTGTTCGGCTGCGCTGGGACCATCCTGTTC
TTCAACCTCTTCCTGGAGCGCATCATCTCGCTGCTGGCCTTCATCATGCGCGCCTGCCGGGAGCGCCAGCTGCGCCGCAG
CGGCCTGCTGCCCGCCACCTTCCGCCGCGGCTCCGCGCTCTCGGAGGCCGACAGCCTGGCGGGCTGGAAGCCCTCGGTGT
ACCACGTGCTGCTCATCCTGGGCCTGTTCGCCGTGCTGCTGTCCTGCTGCGCCTCGGCCATGTACACCAGCGTGGAGGGC
TGGGACTACGTGGACTCGCTCTACTTCTGCTTCGTCACCTTCAGCACCATCGGCTTCGGGGACCTGGTGAGCAGCCAGCA
CGCCGCCTACCGGAACCAGGGGCTCTACCGCCTGGGCAACTTCCTCTTCATCCTGCTCGGCGTGTGCTGCATTTACTCGC
TCTTCAACGTCATCTCCATCCTCATCAAGCAGGTGCTCAACTGGATGCTGCGCAAGCTGAGCTGCCGCTGCTGCGCGCGC
TGCTGCCCGGCTCCTGGCGCGCCCCTGGCCCGGCGCAATGCCATCACCCCAGGCTCCCGGCTGCGCCGCCGCCTGGCCGC
GCTCGGCGCCGACCCCGCGGCCCGCGACAGCGACGCCGAGGGCCGCCGCCTCTCGGGCGAGCTCATCTCCATGCGCGACC
TCACGGCCTCCAACAAGGTGTCGCTGGCGCTGCTGCAGAAGCAGCTGTCGGAGACGGCCAACGGCTACCCGCGCAGCGTG
TGCGTCAACACGCGCCAGAACGGCTTCTCGGGCGGCGTGGGCGCGCTGGGCATCATGAACAACCGGCTGGCCGAGACCAG
CGCCTCCAGGACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
CCGCCGTTCGCACCTCAACGAGGACACCGGCCGCTTCGTGCTGCTGGCGGCGCTCATCGGCCTCTACCTGGTGGCGGGTG
CCACAGTCTTCTCGGCGCTCGAGAGCCCCGGCGAGGCGGAGGCGCGGGCGCGCTGGGGCGCCACGCTGCGCAACTTCAGC
GCTGCGCACGGCGTGGCCGAGCCAGAGCTGCGCGCCTTCCTCCGGCACTACGAGGCCGCGCTGGCCGCCGGCGTCCGCGC
CGACGCGCTGCGCCCGCGCTGGGACTTCCCCGGCGCCTTCTACTTCGTGGGCACCGTGGTGTCAACCATAGGTTTCGGCA
TGACCACCCCCGCGACGGTGGGCGGGAAGGCCTTCCTCATCGCCTACGGGCTGTTCGGCTGCGCTGGGACCATCCTGTTC
TTCAACCTCTTCCTGGAGCGCATCATCTCGCTGCTGGCCTTCATCATGCGCGCCTGCCGGGAGCGCCAGCTGCGCCGCAG
CGGCCTGCTGCCCGCCACCTTCCGCCGCGGCTCCGCGCTCTCGGAGGCCGACAGCCTGGCGGGCTGGAAGCCCTCGGTGT
ACCACGTGCTGCTCATCCTGGGCCTGTTCGCCGTGCTGCTGTCCTGCTGCGCCTCGGCCATGTACACCAGCGTGGAGGGC
TGGGACTACGTGGACTCGCTCTACTTCTGCTTCGTCACCTTCAGCACCATCGGCTTCGGGGACCTGGTGAGCAGCCAGCA
CGCCGCCTACCGGAACCAGGGGCTCTACCGCCTGGGCAACTTCCTCTTCATCCTGCTCGGCGTGTGCTGCATTTACTCGC
TCTTCAACGTCATCTCCATCCTCATCAAGCAGGTGCTCAACTGGATGCTGCGCAAGCTGAGCTGCCGCTGCTGCGCGCGC
TGCTGCCCGGCTCCTGGCGCGCCCCTGGCCCGGCGCAATGCCATCACCCCAGGCTCCCGGCTGCGCCGCCGCCTGGCCGC
GCTCGGCGCCGACCCCGCGGCCCGCGACAGCGACGCCGAGGGCCGCCGCCTCTCGGGCGAGCTCATCTCCATGCGCGACC
TCACGGCCTCCAACAAGGTGTCGCTGGCGCTGCTGCAGAAGCAGCTGTCGGAGACGGCCAACGGCTACCCGCGCAGCGTG
TGCGTCAACACGCGCCAGAACGGCTTCTCGGGCGGCGTGGGCGCGCTGGGCATCATGAACAACCGGCTGGCCGAGACCAG
CGCCTCCAGGACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG
Media and Files
Snapgene File:
Basic Information
Gene Taxonomy:
Construct ID:
IDG_KCNK12_UE_1
Virus Type:
Selection:
Puro,Amp
Vector:
783_hU6_EGFP
Passed QC:
On
Vector Type:
Sequence
Inserted_sequence:
GGCGGTAGCAGGGAAAGGAA
Media and Files
Vector Map:
Snapgene File: