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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNS2-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGACCGGCCAGAGCCTGTGGGACGTGTCGGAGGCTAACGTCGAGGACGGGGAGATCCGCATCAATGTGGGCGGCTTCAA
GAGGAGGCTGCGCTCGCACACGCTGCTGCGCTTCCCCGAGACGCGCCTGGGCCGCTTGCTGCTCTGCCACTCGCGCGAGG
CCATTCTGGAGCTCTGCGATGACTACGACGACGTCCAGCGGGAGTTCTACTTCGACCGCAACCCTGAGCTCTTCCCCTAC
GTGCTGCATTTCTATCACACCGGCAAGCTTCACGTCATGGCTGAGCTATGTGTCTTCTCCTTCAGCCAGGAGATCGAGTA
CTGGGGCATCAACGAGTTCTTCATTGACTCCTGCTGCAGCTACAGCTACCATGGCCGCAAAGTAGAGCCCGAGCAGGAGA
AGTGGGACGAGCAGAGTGACCAGGAGAGCACCACGTCTTCCTTCGATGAGATCCTTGCCTTCTACAACGACGCCTCCAAG
TTCGATGGGCAGCCCCTCGGCAACTTCCGCAGGCAGCTGTGGCTGGCGCTGGACAACCCCGGCTACTCAGTGCTGAGCAG
GGTCTTCAGCATCCTGTCCATCCTGGTGGTGATGGGGTCCATCATCACCATGTGCCTCAATAGCCTGCCCGATTTCCAAA
TCCCTGACAGCCAGGGCAACCCTGGCGAGGACCCTAGGTTCGAAATCGTGGAGCACTTTGGCATTGCCTGGTTCACATTT
GAGCTGGTGGCCAGGTTTGCTGTGGCCCCTGACTTCCTCAAGTTCTTCAAGAATGCCCTAAACCTTATTGACCTCATGTC
CATCGTCCCCTTTTACATCACTCTGGTGGTGAACCTGGTGGTGGAGAGCACACCTACTTTAGCCAACTTGGGCAGGGTGG
CCCAGGTCCTGAGGCTGATGCGGATCTTCCGCATCTTAAAGCTGGCCAGGCACTCCACTGGCCTCCGCTCCCTGGGGGCC
ACTTTGAAATACAGCTACAAAGAAGTAGGGCTGCTCTTGCTCTACCTCTCCGTGGGGATTTCCATCTTCTCCGTGGTGGC
CTACACCATTGAAAAGGAGGAGAACGAGGGCCTGGCCACCATCCCTGCCTGCTGGTGGTGGGCTACCGTCAGTATGACCA
CAGTGGGGTACGGGGATGTGGTCCCAGGGACCACGGCAGGAAAGCTGACTGCCTCTGCCTGCATCTTGGCAGGCATCCTC
GTGGTGGTCCTGCCCATCACCTTGATCTTCAATAAGTTCTCCCACTTTTACCGGCGCCAAAAGCAACTTGAGAGTGCCAT
GCGCAGCTGTGACTTTGGAGATGGAATGAAGGAGGTCCCTTCGGTCAATTTAAGGGACTATTATGCCCATAAAGTTAAAT
CCCTTATGGCAAGCCTGACGAACATGAGCAGGAGCTCACCAAGTGAACTCAGTTTAAATGATTCCCTACGTTACCAAACT
TTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNS2_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGACCGGCCAGAGCCTGTGGGACGTGTCGGAGGCTAACGTCGAGGACGGGGAGATCCGCATCAATGTGGGCGGCTTCAA
GAGGAGGCTGCGCTCGCACACGCTGCTGCGCTTCCCCGAGACGCGCCTGGGCCGCTTGCTGCTCTGCCACTCGCGCGAGG
CCATTCTGGAGCTCTGCGATGACTACGACGACGTCCAGCGGGAGTTCTACTTCGACCGCAACCCTGAGCTCTTCCCCTAC
GTGCTGCATTTCTATCACACCGGCAAGCTTCACGTCATGGCTGAGCTATGTGTCTTCTCCTTCAGCCAGGAGATCGAGTA
CTGGGGCATCAACGAGTTCTTCATTGACTCCTGCTGCAGCTACAGCTACCATGGCCGCAAAGTAGAGCCCGAGCAGGAGA
AGTGGGACGAGCAGAGTGACCAGGAGAGCACCACGTCTTCCTTCGATGAGATCCTTGCCTTCTACAACGACGCCTCCAAG
TTCGATGGGCAGCCCCTCGGCAACTTCCGCAGGCAGCTGTGGCTGGCGCTGGACAACCCCGGCTACTCAGTGCTGAGCAG
GGTCTTCAGCATCCTGTCCATCCTGGTGGTGATGGGGTCCATCATCACCATGTGCCTCAATAGCCTGCCCGATTTCCAAA
TCCCTGACAGCCAGGGCAACCCTGGCGAGGACCCTAGGTTCGAAATCGTGGAGCACTTTGGCATTGCCTGGTTCACATTT
GAGCTGGTGGCCAGGTTTGCTGTGGCCCCTGACTTCCTCAAGTTCTTCAAGAATGCCCTAAACCTTATTGACCTCATGTC
CATCGTCCCCTTTTACATCACTCTGGTGGTGAACCTGGTGGTGGAGAGCACACCTACTTTAGCCAACTTGGGCAGGGTGG
CCCAGGTCCTGAGGCTGATGCGGATCTTCCGCATCTTAAAGCTGGCCAGGCACTCCACTGGCCTCCGCTCCCTGGGGGCC
ACTTTGAAATACAGCTACAAAGAAGTAGGGCTGCTCTTGCTCTACCTCTCCGTGGGGATTTCCATCTTCTCCGTGGTGGC
CTACACCATTGAAAAGGAGGAGAACGAGGGCCTGGCCACCATCCCTGCCTGCTGGTGGTGGGCTACCGTCAGTATGACCA
CAGTGGGGTACGGGGATGTGGTCCCAGGGACCACGGCAGGAAAGCTGACTGCCTCTGCCTGCATCTTGGCAGGCATCCTC
GTGGTGGTCCTGCCCATCACCTTGATCTTCAATAAGTTCTCCCACTTTTACCGGCGCCAAAAGCAACTTGAGAGTGCCAT
GCGCAGCTGTGACTTTGGAGATGGAATGAAGGAGGTCCCTTCGGTCAATTTAAGGGACTATTATGCCCATAAAGTTAAAT
CCCTTATGGCAAGCCTGACGAACATGAGCAGGAGCTCACCAAGTGAACTCAGTTTAAATGATTCCCTACGTTACCAAACT
TTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

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