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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-KCNS3-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGTGTTTGGTGAGTTTTTCCATCGCCCTGGACAAGACGAGGAACTTGTCAACCTGAATGTGGGGGGCTTTAAGCAGTC
TGTTGACCAAAGCACCCTCCTGCGGTTTCCTCACACCAGACTGGGGAAGCTGCTTACTTGCCATTCTGAAGAGGCCATTC
TGGAGCTGTGTGATGATTACAGTGTGGCCGATAAGGAATACTACTTTGATCGGAATCCCTCCTTGTTCAGATATGTTTTG
AATTTTTATTACACGGGGAAGCTGCATGTCATGGAGGAGCTGTGCGTATTCTCATTCTGCCAGGAGATCGAGTACTGGGG
CATCAACGAGCTCTTCATTGATTCTTGCTGCAGCAATCGCTACCAGGAACGCAAGGAGGAAAACCACGAGAAGGACTGGG
ACCAGAAAAGCCATGATGTGAGTACCGACTCCTCGTTTGAAGAGTCGTCTCTGTTTGAGAAAGAGCTGGAGAAGTTTGAC
ACACTGCGATTTGGTCAGCTCCGGAAGAAAATCTGGATTAGAATGGAGAATCCAGCGTACTGCCTGTCCGCTAAGCTTAT
CGCTATCTCCTCCTTGAGCGTGGTGCTGGCCTCCATCGTGGCCATGTGCGTTCACAGCATGTCGGAGTTCCAGAATGAGG
ATGGAGAAGTGGATGATCCGGTGCTGGAAGGATTGGAGATCGCGTGCATTGCCTGGTTCACCGGGGAGCTTGCCGTCCGG
CTGGCTGCCGCTCCTTGTCAAAAGAAATTCTGGAAAAACCCTCTGAACATCATTGACTTTGTCTCTATTATTCCCTTCTA
TGCCACGTTGGCTGTAGACACCAAGGAGGAAGAGAGTGAGGATATTGAGAACATGGGCAAGGTGGTCCAGATCCTACGGC
TTATGAGGATTTTCCGAATTCTAAAGCTTGCCCGGCACTCGGTAGGACTTCGGTCTCTAGGTGCCACACTGAGACACAGC
TACCATGAAGTTGGGCTTCTGCTTCTCTTCCTCTCTGTGGGCATTTCCATTTTCTCTGTGCTTATCTACTCCGTGGAGAA
AGATGACCACACATCCAGCCTCACCAGCATCCCCATCTGCTGGTGGTGGGCCACCATCAGCATGACAACTGTGGGCTATG
GAGACACCCACCCGGTCACCTTGGCGGGAAAGCTCATCGCCAGCACATGCATCATCTGTGGCATCTTGGTGGTGGCCCTT
CCCATCACCATCATCTTCAACAAGTTTTCCAAGTACTACCAGAAGCAAAAGGACATTGATGTGGACCAGTGCAGTGAGGA
TGCACCAGAGAAGTGTCATGAGCTACCTTACTTTAACATTAGGGATATATATGCACAGCGGATGCACGCCTTCATTACCA
GTCTCTCTTCTGTAGGCATTGTGGTGAGCGATCCTGACTCCACAGATGCTTCAAGCATTGAAGACAATGAGGACATTTGT
AACACCACCTCCTTGGAGAATTGCACAGCAAAATGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_KCNS3_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGGTGTTTGGTGAGTTTTTCCATCGCCCTGGACAAGACGAGGAACTTGTCAACCTGAATGTGGGGGGCTTTAAGCAGTC
TGTTGACCAAAGCACCCTCCTGCGGTTTCCTCACACCAGACTGGGGAAGCTGCTTACTTGCCATTCTGAAGAGGCCATTC
TGGAGCTGTGTGATGATTACAGTGTGGCCGATAAGGAATACTACTTTGATCGGAATCCCTCCTTGTTCAGATATGTTTTG
AATTTTTATTACACGGGGAAGCTGCATGTCATGGAGGAGCTGTGCGTATTCTCATTCTGCCAGGAGATCGAGTACTGGGG
CATCAACGAGCTCTTCATTGATTCTTGCTGCAGCAATCGCTACCAGGAACGCAAGGAGGAAAACCACGAGAAGGACTGGG
ACCAGAAAAGCCATGATGTGAGTACCGACTCCTCGTTTGAAGAGTCGTCTCTGTTTGAGAAAGAGCTGGAGAAGTTTGAC
ACACTGCGATTTGGTCAGCTCCGGAAGAAAATCTGGATTAGAATGGAGAATCCAGCGTACTGCCTGTCCGCTAAGCTTAT
CGCTATCTCCTCCTTGAGCGTGGTGCTGGCCTCCATCGTGGCCATGTGCGTTCACAGCATGTCGGAGTTCCAGAATGAGG
ATGGAGAAGTGGATGATCCGGTGCTGGAAGGATTGGAGATCGCGTGCATTGCCTGGTTCACCGGGGAGCTTGCCGTCCGG
CTGGCTGCCGCTCCTTGTCAAAAGAAATTCTGGAAAAACCCTCTGAACATCATTGACTTTGTCTCTATTATTCCCTTCTA
TGCCACGTTGGCTGTAGACACCAAGGAGGAAGAGAGTGAGGATATTGAGAACATGGGCAAGGTGGTCCAGATCCTACGGC
TTATGAGGATTTTCCGAATTCTAAAGCTTGCCCGGCACTCGGTAGGACTTCGGTCTCTAGGTGCCACACTGAGACACAGC
TACCATGAAGTTGGGCTTCTGCTTCTCTTCCTCTCTGTGGGCATTTCCATTTTCTCTGTGCTTATCTACTCCGTGGAGAA
AGATGACCACACATCCAGCCTCACCAGCATCCCCATCTGCTGGTGGTGGGCCACCATCAGCATGACAACTGTGGGCTATG
GAGACACCCACCCGGTCACCTTGGCGGGAAAGCTCATCGCCAGCACATGCATCATCTGTGGCATCTTGGTGGTGGCCCTT
CCCATCACCATCATCTTCAACAAGTTTTCCAAGTACTACCAGAAGCAAAAGGACATTGATGTGGACCAGTGCAGTGAGGA
TGCACCAGAGAAGTGTCATGAGCTACCTTACTTTAACATTAGGGATATATATGCACAGCGGATGCACGCCTTCATTACCA
GTCTCTCTTCTGTAGGCATTGTGGTGAGCGATCCTGACTCCACAGATGCTTCAAGCATTGAAGACAATGAGGACATTTGT
AACACCACCTCCTTGGAGAATTGCACAGCAAAATGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGTAG

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