KCNS3 | Dark Matter

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGTGTTTGGTGAGTTTTTCCATCGCCCTGGACAAGACGAGGAACTTGTCAACCTGAATGTGGGGGGCTTTAAGCAGTC
TGTTGACCAAAGCACCCTCCTGCGGTTTCCTCACACCAGACTGGGGAAGCTGCTTACTTGCCATTCTGAAGAGGCCATTC
TGGAGCTGTGTGATGATTACAGTGTGGCCGATAAGGAATACTACTTTGATCGGAATCCCTCCTTGTTCAGATATGTTTTG
AATTTTTATTACACGGGGAAGCTGCATGTCATGGAGGAGCTGTGCGTATTCTCATTCTGCCAGGAGATCGAGTACTGGGG
CATCAACGAGCTCTTCATTGATTCTTGCTGCAGCAATCGCTACCAGGAACGCAAGGAGGAAAACCACGAGAAGGACTGGG
ACCAGAAAAGCCATGATGTGAGTACCGACTCCTCGTTTGAAGAGTCGTCTCTGTTTGAGAAAGAGCTGGAGAAGTTTGAC
ACACTGCGATTTGGTCAGCTCCGGAAGAAAATCTGGATTAGAATGGAGAATCCAGCGTACTGCCTGTCCGCTAAGCTTAT
CGCTATCTCCTCCTTGAGCGTGGTGCTGGCCTCCATCGTGGCCATGTGCGTTCACAGCATGTCGGAGTTCCAGAATGAGG
ATGGAGAAGTGGATGATCCGGTGCTGGAAGGATTGGAGATCGCGTGCATTGCCTGGTTCACCGGGGAGCTTGCCGTCCGG
CTGGCTGCCGCTCCTTGTCAAAAGAAATTCTGGAAAAACCCTCTGAACATCATTGACTTTGTCTCTATTATTCCCTTCTA
TGCCACGTTGGCTGTAGACACCAAGGAGGAAGAGAGTGAGGATATTGAGAACATGGGCAAGGTGGTCCAGATCCTACGGC
TTATGAGGATTTTCCGAATTCTAAAGCTTGCCCGGCACTCGGTAGGACTTCGGTCTCTAGGTGCCACACTGAGACACAGC
TACCATGAAGTTGGGCTTCTGCTTCTCTTCCTCTCTGTGGGCATTTCCATTTTCTCTGTGCTTATCTACTCCGTGGAGAA
AGATGACCACACATCCAGCCTCACCAGCATCCCCATCTGCTGGTGGTGGGCCACCATCAGCATGACAACTGTGGGCTATG
GAGACACCCACCCGGTCACCTTGGCGGGAAAGCTCATCGCCAGCACATGCATCATCTGTGGCATCTTGGTGGTGGCCCTT
CCCATCACCATCATCTTCAACAAGTTTTCCAAGTACTACCAGAAGCAAAAGGACATTGATGTGGACCAGTGCAGTGAGGA
TGCACCAGAGAAGTGTCATGAGCTACCTTACTTTAACATTAGGGATATATATGCACAGCGGATGCACGCCTTCATTACCA
GTCTCTCTTCTGTAGGCATTGTGGTGAGCGATCCTGACTCCACAGATGCTTCAAGCATTGAAGACAATGAGGACATTTGT
AACACCACCTCCTTGGAGAATTGCACAGCAAAATGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGTAG