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Basic Information

Parent Cell Line:
HEK293
Gene Taxonomy:
Cell Line Taxonomy:
Cell Type:
Stable overexpression cell line
Cell ID:
IDG-HEK293T-PANX2-V5-OE
Selection:
Blasticidin to a final concentration of 10μg/m
Organism:
Homo sapiens, human
Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGTACGTGCCCGCGCTGGGCTGGGAGTTCCTGGCCTCCACGCGCCTCACCTCCGAGCTCAACTTCCTGCTGCAGGAGAT
CGACAACTGTTACCACCGGGCGGCCGAGGGCCGCGCGCCCAAGATCGAGAAGCAGATCCAGTCCAAGGGCCCGGGCATCA
CGGAGCGCGAGAAGCGCGAGATCATCGAGAACGCGGAGAAGGAGAAGAGCCCGGAGCAGAACCTGTTCGAGAAGTACCTG
GAGCGCCGCGGCCGCAGCAACTTCCTGGCCAAGCTGTACCTGGCGCGGCACGTGCTGATCCTGCTGCTGAGCGCCGTGCC
CATCTCCTACCTGTGCACCTACTACGCCACGCAGAAGCAGAACGAGTTCACCTGCGCGCTGGGCGCGTCCCCGGACGGGG
CGGCAGGTGCGGGGCCCGCGGTGCGCGTGAGCTGCAAGCTCCCGTCCGTGCAACTGCAGCGCATCATCGCGGGCGTGGAC
ATCGTGCTGCTGTGCGTCATGAACCTCATCATCCTCGTCAACCTCATCCACCTCTTCATCTTCCGCAAGAGCAACTTCAT
CTTCGACAAGCTGCACAAGGTGGGCATCAAGACGCGCCGGCAGTGGCGCCGCTCGCAGTTCTGCGACATCAACATCCTGG
CCATGTTCTGCAACGAGAACCGCGACCACATCAAGTCGCTCAACCGGCTGGACTTCATCACCAACGAGAGCGACCTCATG
TACGACAACGTGGTCCGGCAGCTGCTGGCGGCGCTGGCGCAGTCCAACCACGACGCCACCCCCACGGTGCGCGACTCGGG
GGTGCAGACCGTGGACCCCAGCGCCAACCCCGCCGAGCCCGACGGCGCCGCCGAGCCGCCCGTGGTCAAGCGGCCGCGCA
AGAAGATGAAGTGGATCCCCACCAGCAACCCGCTTCCGCAGCCCTTCAAGGAGCCGCTGGCCATCATGCGCGTGGAGAAC
AGCAAGGCGGAGAAGCCGAAGCCCGCGCGCAGGAAGACGGCCACGGACACGCTGATCGCGCCGCTGCTGGACCGCTCCGC
CCACCACTACAAGGGCGGAGGGGGCGACCCGGGCCCCGGCCCCGCCCCTGCCCCCGCCCCGCCGCCCGCCCCCGACAAGA
AGCACGCGCGCCACTTCTCCCTGGACGTGCACCCCTACATCCTCGGCACCAAGAAGGCCAAGGCCGAGGCGGTGCCCGCC
GCCCTGCCCGCCTCCCGGAGCCAGGAGGGGGGCTTCCTGTCCCAGGCGGAGGACTGTGGGCTGGGCCTGGCCCCGGCGCC
CATCAAAGATGCTCCGCTCCCCGAGAAGGAAATCCCGTACCCCACAGAGCCAGCCCGGGCAGGGCTTCCCTCGGGGGGCC
CGTTCCACGTCCGCTCACCTCCCGCCGCCCCTGCTGTGGCCCCTCTGACACCAGCCAGCCTGGGCAAGGCGGAGCCCCTC
ACCATCCTGAGCCGAAACGCCACACACCCGCTGCTGCACATCAACACGCTGTACGAGGCCCGGGAGGAGGAGGACGGGGG
CCCCCGCCTGCCGCAGGACGTGGGGGACCTCATCGCCATCCCTGCCCCACAGCAGATCCTCATCGCCACCTTCGACGAGC
CGAGAACGGTCGTGAGTACTGTGGAGTTTCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTC
GGTCTCGATTCTACGTAG

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Basic Information

Gene Taxonomy:
Virus Type:
Vector Type:
Construct ID:
IDG_PANX2_OE_1

Selection:
Amp, Blasticidin

Vector:
lPX304

Construct Type:
Expression

Tag:
C terminal V5

Passed QC:
Yes

Sequence

Inserted_sequence:
ATGTACGTGCCCGCGCTGGGCTGGGAGTTCCTGGCCTCCACGCGCCTCACCTCCGAGCTCAACTTCCTGCTGCAGGAGAT
CGACAACTGTTACCACCGGGCGGCCGAGGGCCGCGCGCCCAAGATCGAGAAGCAGATCCAGTCCAAGGGCCCGGGCATCA
CGGAGCGCGAGAAGCGCGAGATCATCGAGAACGCGGAGAAGGAGAAGAGCCCGGAGCAGAACCTGTTCGAGAAGTACCTG
GAGCGCCGCGGCCGCAGCAACTTCCTGGCCAAGCTGTACCTGGCGCGGCACGTGCTGATCCTGCTGCTGAGCGCCGTGCC
CATCTCCTACCTGTGCACCTACTACGCCACGCAGAAGCAGAACGAGTTCACCTGCGCGCTGGGCGCGTCCCCGGACGGGG
CGGCAGGTGCGGGGCCCGCGGTGCGCGTGAGCTGCAAGCTCCCGTCCGTGCAACTGCAGCGCATCATCGCGGGCGTGGAC
ATCGTGCTGCTGTGCGTCATGAACCTCATCATCCTCGTCAACCTCATCCACCTCTTCATCTTCCGCAAGAGCAACTTCAT
CTTCGACAAGCTGCACAAGGTGGGCATCAAGACGCGCCGGCAGTGGCGCCGCTCGCAGTTCTGCGACATCAACATCCTGG
CCATGTTCTGCAACGAGAACCGCGACCACATCAAGTCGCTCAACCGGCTGGACTTCATCACCAACGAGAGCGACCTCATG
TACGACAACGTGGTCCGGCAGCTGCTGGCGGCGCTGGCGCAGTCCAACCACGACGCCACCCCCACGGTGCGCGACTCGGG
GGTGCAGACCGTGGACCCCAGCGCCAACCCCGCCGAGCCCGACGGCGCCGCCGAGCCGCCCGTGGTCAAGCGGCCGCGCA
AGAAGATGAAGTGGATCCCCACCAGCAACCCGCTTCCGCAGCCCTTCAAGGAGCCGCTGGCCATCATGCGCGTGGAGAAC
AGCAAGGCGGAGAAGCCGAAGCCCGCGCGCAGGAAGACGGCCACGGACACGCTGATCGCGCCGCTGCTGGACCGCTCCGC
CCACCACTACAAGGGCGGAGGGGGCGACCCGGGCCCCGGCCCCGCCCCTGCCCCCGCCCCGCCGCCCGCCCCCGACAAGA
AGCACGCGCGCCACTTCTCCCTGGACGTGCACCCCTACATCCTCGGCACCAAGAAGGCCAAGGCCGAGGCGGTGCCCGCC
GCCCTGCCCGCCTCCCGGAGCCAGGAGGGGGGCTTCCTGTCCCAGGCGGAGGACTGTGGGCTGGGCCTGGCCCCGGCGCC
CATCAAAGATGCTCCGCTCCCCGAGAAGGAAATCCCGTACCCCACAGAGCCAGCCCGGGCAGGGCTTCCCTCGGGGGGCC
CGTTCCACGTCCGCTCACCTCCCGCCGCCCCTGCTGTGGCCCCTCTGACACCAGCCAGCCTGGGCAAGGCGGAGCCCCTC
ACCATCCTGAGCCGAAACGCCACACACCCGCTGCTGCACATCAACACGCTGTACGAGGCCCGGGAGGAGGAGGACGGGGG
CCCCCGCCTGCCGCAGGACGTGGGGGACCTCATCGCCATCCCTGCCCCACAGCAGATCCTCATCGCCACCTTCGACGAGC
CGAGAACGGTCGTGAGTACTGTGGAGTTTCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTC
GGTCTCGATTCTACGTAG

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