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Data and Resources for PKD1L2 Gene

Disease Associated Data

Basic Information

Gene Taxonomy:

UniprotID:

Q7Z442

Mouse Homolog:

Pkd1l2

Pharos Link:

Knockout Mouse Phenotype:

Abnormality of the lens

Female infertility

Monogenic Disease Association:

Polygenic Disease Association:

diabetic nephropathies

liver cirrhosis

platelet function tests

10K Immunome:

SARS-CoV-2 Association:

SARS-CoV-2 Vaccine Association:

Basic Information

Gene Taxonomy:

UniprotID:

Q7Z442

Mouse Homolog:

Pkd1l2

Pharos Link:

Knockout Mouse Phenotype:

Abnormality of the lens

Female infertility

Monogenic Disease Association:

Polygenic Disease Association:

diabetic nephropathies

liver cirrhosis

platelet function tests

10K Immunome:

SARS-CoV-2 Association:

SARS-CoV-2 Vaccine Association:

Over Expression Cell Lines

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

Cell Line Taxonomy:

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-PKD1L2-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGGGGGAGGACTCTCCAGTGGCTATGTTCAGCTGGTATTTGGACAACACCCCAACAGAGCAGGCTGAGCCCCTCCCGGA
TGCCTGCAGACTCAGAGGATTTTGGCCAAGGTCCTTAACCCTCCTCCAGAGCAACACCTCCACGTTGCTGTTGAACAGCT
CGTTTCTGCAGTCCCGGGGAGAGGTCATCCGAATCAGAGCCACAGCACTGACCAGGCATGCCTATGGGGAGGACACCTAT
GTGATCAGCACTGTGCCTCCCCGTGAGGTGCCTGCCTGCACTATTGCCCCAGAGGAGGGCACCGTTCTGACGAGCTTTGC
CATCTTCTGCAACGCCTCCACAGCCCTGGGACCCCTGGAGTTCTGCTTCTGTCTGGAATCAGGTTCCTGCCTACACTGTG
GCCCTGAACCTGCCCTCCCATCAGTGTATCTGCCACTTGGAGAGGAGAACAATGACTTTGTGCTGACAGTAGTTATTTCT
GCCACCAATCGTGCAGGGGACACGCAGCAGACCCAGGCCATGGCTAAGGTGGCACTCGGAGACACATGTGTTGAGGATGT
AGCATTCCAGGCTGCCGTGTCAGAGAAAATCCCCACAGCTCTGCAAGGCGAGGGTGGCCCCGAGCAGCTCCTCCAGCTGG
CCAAGGCTGTGTCCTCCATGCTGAACCAAGAGCATGAAAGCCAGGGCTCAGGACAGTCACTGAGCATAGACGTCAGACAG
AAGGTCAGAGAGCATGTGCTGGGATCACTGTCTGCAGTCACCACCGGCTTGGAGGACGTGCAGAGGGTGCAGGAGCTGGC
CGAGGTGCTGAGAGAGGTGACCTGCCGGAGTAAGGAACTCACACCCTCGGCCCAGGGGTCCTGCATGGGCGATTCATGGG
AAGGTGCCCCTCCTGCTGCCCATGTATCTCACGCTAGGTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCT
AACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:

Virus Type:

Lentiviral Vector

Vector Type:

Construct ID:

IDG_PKD1L2_OE_1

Selection:

Amp, Blasticidin

Vector:

lPX304

Construct Type:

Expression

Tag:

C terminal V5

Passed QC:

Yes

Sequence

Inserted_sequence:

ATGGGGGAGGACTCTCCAGTGGCTATGTTCAGCTGGTATTTGGACAACACCCCAACAGAGCAGGCTGAGCCCCTCCCGGA
TGCCTGCAGACTCAGAGGATTTTGGCCAAGGTCCTTAACCCTCCTCCAGAGCAACACCTCCACGTTGCTGTTGAACAGCT
CGTTTCTGCAGTCCCGGGGAGAGGTCATCCGAATCAGAGCCACAGCACTGACCAGGCATGCCTATGGGGAGGACACCTAT
GTGATCAGCACTGTGCCTCCCCGTGAGGTGCCTGCCTGCACTATTGCCCCAGAGGAGGGCACCGTTCTGACGAGCTTTGC
CATCTTCTGCAACGCCTCCACAGCCCTGGGACCCCTGGAGTTCTGCTTCTGTCTGGAATCAGGTTCCTGCCTACACTGTG
GCCCTGAACCTGCCCTCCCATCAGTGTATCTGCCACTTGGAGAGGAGAACAATGACTTTGTGCTGACAGTAGTTATTTCT
GCCACCAATCGTGCAGGGGACACGCAGCAGACCCAGGCCATGGCTAAGGTGGCACTCGGAGACACATGTGTTGAGGATGT
AGCATTCCAGGCTGCCGTGTCAGAGAAAATCCCCACAGCTCTGCAAGGCGAGGGTGGCCCCGAGCAGCTCCTCCAGCTGG
CCAAGGCTGTGTCCTCCATGCTGAACCAAGAGCATGAAAGCCAGGGCTCAGGACAGTCACTGAGCATAGACGTCAGACAG
AAGGTCAGAGAGCATGTGCTGGGATCACTGTCTGCAGTCACCACCGGCTTGGAGGACGTGCAGAGGGTGCAGGAGCTGGC
CGAGGTGCTGAGAGAGGTGACCTGCCGGAGTAAGGAACTCACACCCTCGGCCCAGGGGTCCTGCATGGGCGATTCATGGG
AAGGTGCCCCTCCTGCTGCCCATGTATCTCACGCTAGGTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCT
AACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:

D.IC57-pLX304-PKD1L2-expression-vector.dna

Constructs for CRISPR Interference

Basic Information

Gene Taxonomy:

Construct ID:

IDG_PKD1L2_UE_1

Virus Type:

Lentiviral Vector

Selection:

Puro,Amp

Vector:

783_hU6_EGFP

Passed QC:

On

Vector Type:

CRISPR Interference

Sequence

Inserted_sequence:

GCTGTGGCTAAGTCAGAAGG

Media and Files

Vector Map:

Snapgene File:

G6-783-PKD1L2-interference-vector.dna

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