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Data and Resources for SCN2B Gene

Disease Associated Data

Basic Information

Gene Taxonomy:

UniprotID:

O60939

Mouse Homolog:

Scn2b

Pharos Link:

Knockout Mouse Phenotype:

Abnormal nervous system electrophysiology

abnormal QT variability

increased susceptibility to pharmacologically induced seizures

obsolete Glucose intolerance

Monogenic Disease Association:

atrial fibrillation

Polygenic Disease Association:

brugada syndrome (disorder)

10K Immunome:

SARS-CoV-2 Association:

SARS-CoV-2 Vaccine Association:

Over Expression Cell Lines

Basic Information

Parent Cell Line:

HEK293

Gene Taxonomy:

Cell Line Taxonomy:

Over Expression Cell Line

Cell Type:

Stable overexpression cell line

Cell ID:

IDG-HEK293T-SCN2B-V5-OE

Selection:

Blasticidin to a final concentration of 10μg/m

Organism:

Homo sapiens, human

Description:

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCACAGAGATGCCTGGCTACCTCGCCCTGCCTTCAGCCTCACGGGGCTCAGTCTCTTTTTCTCTTTGGTGCCACCAGG
ACGGAGCATGGAGGTCACAGTACCTGCCACCCTCAACGTCCTCAATGGCTCTGACGCCCGCCTGCCCTGCACCTTCAACT
CCTGCTACACAGTGAACCACAAACAGTTCTCCCTGAACTGGACTTACCAGGAGTGCAACAACTGCTCTGAGGAGATGTTC
CTCCAGTTCCGCATGAAGATCATTAACCTGAAGCTGGAGCGGTTTCAAGACCGCGTGGAGTTCTCAGGGAACCCCAGCAA
GTACGATGTGTCGGTGATGCTGAGAAACGTGCAGCCGGAGGATGAGGGGATTTACAACTGCTACATCATGAACCCCCCTG
ACCGCCACCGTGGCCATGGCAAGATCCATCTGCAGGTCCTCATGGAAGAGCCCCCTGAGCGGGACTCCACGGTGGCCGTG
ATTGTGGGTGCCTCCGTCGGGGGCTTCCTGGCTGTGGTCATCTTGGTGCTGATGGTGGTCAAGTGTGTGAGGAGAAAAAA
AGAGCAGAAGCTGAGCACAGATGACCTGAAGACCGAGGAGGAGGGCAAGACGGACGGTGAAGGCAACCCGGATGATGGCG
CCAAGTACCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Constructs for Lentiviral Expression

Basic Information

Gene Taxonomy:

Virus Type:

Lentiviral Vector

Vector Type:

Construct ID:

IDG_SCN2B_OE_1

Selection:

Amp, Blasticidin

Vector:

lPX304

Construct Type:

Expression

Tag:

C terminal V5

Passed QC:

Yes

Sequence

Inserted_sequence:

ATGCACAGAGATGCCTGGCTACCTCGCCCTGCCTTCAGCCTCACGGGGCTCAGTCTCTTTTTCTCTTTGGTGCCACCAGG
ACGGAGCATGGAGGTCACAGTACCTGCCACCCTCAACGTCCTCAATGGCTCTGACGCCCGCCTGCCCTGCACCTTCAACT
CCTGCTACACAGTGAACCACAAACAGTTCTCCCTGAACTGGACTTACCAGGAGTGCAACAACTGCTCTGAGGAGATGTTC
CTCCAGTTCCGCATGAAGATCATTAACCTGAAGCTGGAGCGGTTTCAAGACCGCGTGGAGTTCTCAGGGAACCCCAGCAA
GTACGATGTGTCGGTGATGCTGAGAAACGTGCAGCCGGAGGATGAGGGGATTTACAACTGCTACATCATGAACCCCCCTG
ACCGCCACCGTGGCCATGGCAAGATCCATCTGCAGGTCCTCATGGAAGAGCCCCCTGAGCGGGACTCCACGGTGGCCGTG
ATTGTGGGTGCCTCCGTCGGGGGCTTCCTGGCTGTGGTCATCTTGGTGCTGATGGTGGTCAAGTGTGTGAGGAGAAAAAA
AGAGCAGAAGCTGAGCACAGATGACCTGAAGACCGAGGAGGAGGGCAAGACGGACGGTGAAGGCAACCCGGATGATGGCG
CCAAGTACCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGTAG

Media and Files

Snapgene File:

D.IC10-pLX304-SCN2B-expression-vector.dna

Constructs for CRISPR Interference

Basic Information

Gene Taxonomy:

Construct ID:

IDG_SCN2B_UE_1

Virus Type:

Lentiviral Vector

Selection:

Puro,Amp

Vector:

783_hU6_EGFP

Passed QC:

On

Vector Type:

CRISPR Interference

Sequence

Inserted_sequence:

GGGACCAAGGACCACAAGGT

Media and Files

Vector Map:

Snapgene File:

G10-783-SCN2B-interference-vector.dna

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