This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.
ATGCACGTGAAGAAGTACCTGCTGAAGGGCCTGCATCGGCTGCAGAAGGGCCCCGGCTACACGTACAAGGAGCTGCTGGT
GTGGTACTGCGACAACACCAACACCCACGGCCCCAAGCGCACCATCTGTGAGGGGCCCAAGAAGAAAGCCATGTGGTTCC
TGCTCACCCTGCTCTTCGCCGCCCTCGTCTGCTGGCAGTGGGGCATCTTCATCAGGACCTACTTGAGCTGGGAGGTCAGC
GTCTCCCTCTCCGTAGGCTTCAAGACCATGGACTTCCCTGCCGTCACCATCTGCAATGCTAGCCCCTTCAAGTATTCCAA
AATCAAGCATTTGCTGAAGGACCTGGATGAGCTGATGGAAGCTGTCCTGGAGAGAATCCTGGCTCCTGAGCTAAGCCATG
CCAATGCCACCAGGAACCTGAACTTCTCCATCTGGAACCACACACCCCTGGTCCTTATTGATGAACGGAACCCCCACCAC
CCCATGGTCCTTGATCTCTTTGGAGACAACCACAATGGCTTAACAAGCAGCTCAGCATCAGAAAAGATCTGTAATGCCCA
CGGGTGCAAAATGGCCATGAGACTATGTAGCCTCAACAGGACCCAGTGTACCTTCCGGAACTTCACCAGTGCTACCCAGG
CATTGACAGAGTGGTACATCCTGCAGGCCACCAACATCTTTGCACAGGTGCCACAGCAGGAGCTAGTAGAGATGAGCTAC
CCCGGCGAGCAGATGATCCTGGCCTGCCTATTCGGAGCTGAGCCCTGCAACTACCGGAACTTCACGTCCATCTTCTACCC
TCACTATGGCAACTGTTACATCTTCAACTGGGGCATGACAGAGAAGGCACTTCCTTCGGCCAACCCTGGAACTGAATTCG
GCCTGAAGTTGATCCTGGACATAGGCCAGGAAGACTACGTCCCCTTCCTTGCGTCCACGGCCGGGGTCAGGCTGATGCTT
CACGAGCAGAGGTCATACCCCTTCATCAGAGATGAGGGCATCTACGCCATGTCGGGGACAGAGACGTCCATCGGGGTACT
CGTGGACAAGCTTCAGCGCATGGGGGAGCCCTACAGCCCGTGCACCGTGAATGGTTCTGAGGTCCCCGTCCAAAACTTCT
ACAGTGACTACAACACGACCTACTCCATCCAGGCCTGTCTTCGCTCCTGCTTCCAAGACCACATGATCCGTAACTGCAAC
TGTGGCCACTACCTGTACCCACTGCCCCGTGGGGAGAAATACTGCAACAACCGGGACTTCCCAGACTGGGCCCATTGCTA
CTCAGATCTACAGATGAGCGTGGCGCAGAGAGAGACCTGCATTGGCATGTGCAAGGAGTCCTGCAATGACACCCAGTACA
AGATGACCATCTCCATGGCTGACTGGCCTTCTGAGGCCTCCGAGGACTGGATTTTCCACGTCTTGTCTCAGGAGCGGGAC
CAAAGCACCAATATCACCCTGAGCAGGAAGGGAATTGTCAAGCTCAACATCTACTTCCAAGAATTTAACTATCGCACCAT
TGAAGAATCAGCAGCCAATAACATCGTCTGGCTGCTCTCGAATCTGGGTGGCCAGTTTGGCTTCTGGATGGGGGGCTCTG
TGCTGTGCCTCATCGAGTTTGGGGAGATCATCATCGACTTTGTGTGGATCACCATCATCAAGCTGGTGGCCTTGGCCAAG
AGCCTACGGCAGCGGCGAGCCCAAGCCAGCTACGCTGGCCCACCGCCCACCGTGGCCGAGCTGGTGGAGGCCCACACCAA
CTTTGGCTTCCAGCCTGACACGGCCCCCCGCAGCCCCAACACTGGGCCCTACCCCAGTGAGCAGGCCCTGCCCATCCCAG
GCACCCCGCCCCCCAACTATGACTCCCTGCGTCTGCAGCCGCTGGACGTCATCGAGTCTGACAGTGAGGGTGATGCCATC
TACCCAACTTTCTTGTACAAAGTGGTtggtaagcctatccctaaccctctcctcggtctcgattctacgtag
