TMEM63A | Dark Matter

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGATGGACTCCCCGTTCCTGGAGCTGTGGCAGTCCAAGGCAGTGTCCATCAGGGAGCAGCTGGGACTCGGGGACCGGCC
CAACGACTCCTATTGCTACAACTCGGCCAAAAACAGCACCGTGCTCCAGGGGGTCACCTTTGGTGGCATCCCCACTGTCC
TGCTCATAGACGTCAGCTGCTTCCTGTTCTTAATCTTGGTGTTTTCTATTATAAGAAGAAGATTCTGGGACTATGGCCGC
ATTGCCCTGGTGTCAGAAGCAGACAGCGAGTCCAGATTTCAGAGATTGTCATCGACTTCCTCCTCAGGTCAACAAGACTT
TGAAAATGAGCTGGGATGCTGTCCCTGGCTGACTGCCATCTTCCGTCTGCATGATGACCAGATCCTGGAATGGTGTGGGG
AGGACGCCATCCACTACCTGTCCTTCCAGAGGCACATCATCTTCCTGTTGGTGGTGGTCAGCTTTTTGTCCCTGTGTGTC
ATCCTGCCTGTCAACCTCTCAGGGGACTTGCTGGACAAAGACCCGTATAGTTTTGGGAGGACAACAATAGCAAACCTACA
GACTGACAATGACCTCCTTTGGCTGCACACCATCTTTGCTGTCATTTACCTCTTCCTCACTGTGGGTTTCATGCGGCACC
ACACTCAGTCCATTAAGTACAAAGAGGAGAACCTGGTGAGGCGGACCCTGTTCATCACAGGACTCCCCAGAGATGCCAGG
AAGGAGACTGTGGAGAGCCACTTCCGGGACGCGTATCCCACGTGTGAGGTGGTTGATGTGCAGCTGTGCTACAACGTGGC
CAAACTGATCTACCTGTGCAAGGAGAAAAAGAAGACTGAGAAGAGCCTGACCTATTACACAAACCTGCAGGTGAAGACAG
GCCAGCGGACCCTCATCAACCCCAAGCCCTGTGGCCAGTTTTGCTGCTGTGAAGTGCTGGGCTGTGAGTGGGAAGACGCC
ATCTCTTACTACACACGGATGAAGGACAGGCTGCTGGAGAGGATCACAGAGGAAGAACGCCACGTCCAGGACCAGCCCCT
GGGAATGGCCTTCGTCACCTTCCAGGAGAAGTCCATGGCCACCTACATCCTGAAAGATTTCAATGCCTGCAAGTGTCAGA
GCCTTCAGTGCAAAGGTGAGCCCCAGCCGTCCTCCCATAGCAGGGAGCTCTATACCTCCAAGTGGACAGTCACCTTTGCT
GCTGACCCTGAGGACATCTGCTGGAAGAACCTCTCTATCCAGGGCCTCCGCTGGTGGCTACAGTGGCTGGGCATCAACTT
CACCCTCTTCCTGGGGCTATTTTTCCTGACCACACCCTCCATCATCCTGTCCACCATGGACAAGTTTAATGTCACCAAAC
CCATCCATGCGCTGAATAACCCGATCATCAGCCAGTTCTTCCCCACCCTCCTGCTCTGGTCCTTCTCGGCCCTGCTCCCC
TCCATTGTCTACTACTCTACACTGCTGGAGTCTCACTGGACCAAGTCGGGGGAAAACCAGATCATGATGACCAAAGTCTA
CATATTCTTGATCTTCATGGTGCTGATCCTGCCCTCCCTGGGTCTCACCAGTCTAGATTTTTTCTTCCGGTGGCTCTTTG
ACAAAACTTCCTCGGAGGCCTCCATCAGGTTGGAGTGCGTCTTCCTGCCTGACCAGGGTGCCTTCTTTGTGAACTATGTC
ATCGCCTCGGCCTTCATCGGCAATGGCATGGAGCTGCTGCGGCTGCCAGGTCTCATCCTCTATACCTTCCGCATGATCAT
GGCCAAGACGGCTGCTGACCGCAGGAATGTCAAGCAGAACCAGGCCTTCCAGTACGAGTTTGGAGCCATGTATGCATGGA
TGCTGTGTGTCTTCACTGTCATCGTGGCCTACAGCATCACTTGTCCCATCATCGCGCCATTTGGCCTCATCTACATCCTG
CTCAAGCACATGGTGGACCGGCACAACCTCTACTTCGTCTACCTCCCAGCCAAGCTGGAGAAGGGGATCCACTTTGCCGC
TGTGAACCAGGCCTTGGCAGCCCCCATCCTGTGCCTCTTCTGGCTCTACTTCTTTTCCTTCCTGCGCCTGGGTATGAAGG
CCCCCGCCACTCTGTTCACCTTCCTGGTGCTGCTGCTCACCATCCTGGTCTGCCTGGCTCACACCTGCTTTGGATGCTTC
AAGCACCTCAGCCCTCTGAACTACAAAACAGAAGAGCCTGCAAGTGACAAAGGAAGTGAGGCAGAGGCCCACATGCCCCC
ACCGTTCACACCCTACGTGCCTCGGATTCTGAACGGCTTGGCCTCGGAGAGGACAGCACTGTCTCCGCAGCAGCAGCAGC
AGCAGACCTATGGTGCCATCCACAACATCAGCGGGACTATCCCTGGACAGTGCTTGGCGCAGAGCGCCACGGGCAGTGTG
GCTGCTGCCCCCCAGGAGGCCTGCCCAACTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCT
CGATTCTACGTAG