TMEM63B | Dark Matter

This cell line stably expresses dark ion channel in frame with V5 tag on the C-terminus. The ion channel construct is randomly integrated in the genome using lentiviral transduction. This cell line also expresses gene conferring Blasticidin resistance. These cells lines can be used for various molecular and biochemical characterizations of dark ion channels in vitro.

Sequence

ORF Sequence:

ATGCTGCCCTTTCTGCTGGCCACACTGGGCACCACAGCCCTCAACAACAGCAACCCCAAGGACTACTGCTACAGCGCCCG
CATCCGCAGCACTGTCCTGCAGGGCCTGCCCTTTGGGGGCGTCCCCACCGTGCTGGCTCTCGACTTCATGTGCTTCCTTG
CACTGCTGTTCTTATTCTCTATCCTCCGGAAGGTGGCCTGGGACTATGGGCGGCTGGCCTTGGTGACAGATGCAGACAGG
CTTCGGCGGCAGGAGAGGGACCGAGTGGAACAGGAATATGTGGCTTCAGCTATGCACGGGGACAGCCATGACCGGTATGA
GCGTCTCACCTCTGTCTCCAGCTCCGTTGACTTTGACCAAAGGGACAATGGTTTCTGTTCCTGGCTGACAGCCATCTTCA
GGATAAAGGATGATGAGATCCGGGACAAATGTGGGGGCGATGCCGTGCACTACCTGTCCTTTCAGCGGCACATCATCGGG
CTGCTGGTGGTTGTGGGCGTCCTCTCCGTAGGCATCGTGCTGCCTGTCAACTTCTCAGGGGACCTGCTGGAGAACAATGC
CTACAGCTTTGGGAGAACCACCATTGCCAACTTGAAATCAGGGAACAACCTGCTATGGCTGCACACCTCCTTCGCCTTCC
TGTATCTGCTGCTCACCGTCTACAGCATGCGTAGACACACCTCCAAGATGCGCTACAAGGAGGATGATCTGGTGAAGCGG
ACCCTCTTCATCAATGGAATCTCCAAATATGCAGAGTCAGAAAAGATCAAGAAGCATTTTGAGGAAGCCTACCCCAACTG
CACAGTTCTCGAAGCCCGCCCGTGTTACAACGTGGCTCGCCTAATGTTCCTCGATGCAGAGAGGAAGAAGGCCGAGCGGG
GAAAGCTGTACTTCACAAACCTCCAGAGCAAGGAGAACGTGCCTACCATGATCAACCCCAAGCCCTGTGGCCACCTCTGC
TGCTGTGTGGTGCGAGGCTGTGAGCAGGTGGAGGCCATTGAGTACTACACAAAGCTGGAGCAGAAGCTGAAGGAAGACTA
CAAGCGGGAGAAGGAGAAGGTGAATGAGAAGCCTCTTGGCATGGCCTTTGTCACCTTCCACAATGAGACTATCACCGCCA
TCATCCTGAAGGACTTCAACGTGTGTAAATGCCAGGGCTGCACCTGCCGTGGGGAGCCACGCCCCTCATCCTGCAGCGAG
TCCCTGCACATCTCCAACTGGACCGTGTCCTATGCCCCTGACCCTCAGAACATCTACTGGGAGCACCTCTCCATCCGAGG
CTTCATCTGGTGGCTGCGCTGCCTGGTCATCAATGTCGTCCTCTTCATCCTCCTCTTCTTCCTCACCACTCCAGCCATCA
TCATCACCACCATGGACAAGTTCAACGTCACCAAGCCTGTGGAGTACCTCAACAACCCCATCATCACCCAGTTCTTCCCC
ACCCTGCTGCTGTGGTGCTTCTCGGCCCTCCTTCCCACCATCGTCTACTACTCAGCCTTCTTTGAAGCCCACTGGACACG
CTCTGGGGAGAACAGGACAACCATGCACAAGTGCTACACTTTCCTCATCTTCATGGTGCTGCTCCTACCCTCGCTGGGAC
TGAGCAGCCTGGACCTCTTCTTCCGCTGGCTCTTTGATAAGAAATTCTTGGCTGAGGCAGCTATTCGGTTTGAGTGTGTG
TTCCTGCCCGACAACGGCGCCTTCTTCGTGAACTACGTCATTGCCTCAGCCTTTATCGGCAACGCCATGGACCTGCTGCG
CATCCCAGGCCTGCTCATGTACATGATCCGGCTCTGCCTGGCGCGCTCGGCCGCCGAGAGGCGCAACGTGAAGCGGCATC
AGGCCTACGAGTTCCAGTTTGGCGCAGCCTACGCCTGGATGATGTGCGTCTTCACGGTGGTCATGACCTACAGTATCACC
TGCCCCATCATCGTGCCCTTCGGGCTCATGTACATGCTGCTGAAGCACCTGGTAGACAGGTACAATCTCTACTACGCCTA
CCTGCCGGCCAAGCTGGACAAGAAGATCCACTCGGGGGCTGTGAACCAGGTGGTGGCCGCGCCCATCCTCTGCCTCTTCT
GGCTGCTCTTCTTTTCCACCATGCGCACGGGGTTCCTAGCTCCCACGTCTATGTTCACATTTGTGGTCCTGGTCATCACC
ATCGTCATCTGTCTCTGCCACGTCTGCTTTGGACACTTCAAATACCTCAGTGCCCACAACTACAAGATTGAGCACACGGA
GACAGATACTGTGGACCCCAGAAGCAATGGACGGCCCCCCACTGCTGCTGCTGTCCCCAAATCTGCGAAATACATCGCTC
AGGTGCTGCAGGACTCAGAGGTGGACGGGGATGGGGATGGGGCTCCTGGGAGCTCAGGGGATGAGCCCCCATCATCCTCA
TCCCAAGATGAGGAGTTGCTGATGCCACCCGACGCCCTCACGGACACAGACTTCCAGTCTTGCGAGGACAGCCTCATAGA
GAATGAGATTCACCAGACAGCTTTCTTGTACAAAGTGGTTGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA
CGTAG